上海口腔医学

上海口腔医学 ›› 2025, Vol. 34 ›› Issue (2): 113-118.doi: 10.19439/j.sjos.2025.02.001

• 论著 • 上一篇    下一篇

不同类型脱钙液在口腔颌面部牙-颌骨-牙周联合组织制片中的应用效果评价

顾挺, 王敏, 王瑜, 李江#, 张春叶#   

  1. 上海交通大学医学院附属第九人民医院 口腔病理科,上海交通大学口腔医学院,国家口腔医学中心, 国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海市口腔医学研究所,上海 200011
  • 收稿日期:2023-12-28 修回日期:2024-03-08 出版日期:2025-04-25 发布日期:2025-05-15
  • 通讯作者: 李江,E-mail:lijiang182000@126.com;张春叶,E-mail: yezi1806@126.com。#共同通信作者
  • 作者简介:顾挺（1981-）,男,硕士,主管技师,E-mail: yuemachun@126.com
  • 基金资助:
    国家自然科学基金（82141108,82103405); 上海交通大学医学院附属第九人民医院临床研究助推计划 (JYLJ202119)

A study on the effectiveness of different types of decalcifying solutions in the preparation of combined oral and maxillofacial tooth-mandibular-periodontal tissues

Gu Ting, Wang Min, Wang Yu, Li Jiang, Zhang Chunye   

  1. Department of Oral Pathology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology. Shanghai 200011, China
  • Received:2023-12-28 Revised:2024-03-08 Online:2025-04-25 Published:2025-05-15

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摘要： 目的：对比分析MORSE、甲酸和Plank-Rychlo脱钙液处理牙-颌骨-牙周联合组织的效果,评价其应用价值。方法：收集上海交通大学医学院附属第九人民医院口腔病理科废弃的包括正常牙、颌骨及牙周组织的标本,随机分为3组,分析3种不同脱钙液在脱钙时间、染色效果及核酸完整性方面的差异。采用SPSS 23.0软件包对数据进行统计学分析。结果：MORSE组、甲酸组和Plank-Rychlo组的平均脱钙时间分别为4.5、3.5和3.6天,H-E染色评分从高至低依次为MORSE组、甲酸组和Plank-Rychlo组。免疫组织化学染色显示,MORSE组中AE1/AE3、p63和Ki67得分优于甲酸组间和Plank-Rychlo组,3组Vimentin染色指数相比无显著差异。荧光原位杂交结果显示,MORSE组15例、甲酸组1例和Plank-Rychlo组4例检测到正常荧光信号。DNA浓度均值在MORSE组、甲酸组和Plank-Rychlo组分别为1.987、1.963和1.115 ng/μL,RNA浓度均值分别为13.03、11.08和1.66 ng/μL;MORSE组和甲酸组间DNA浓度和RNA浓度相比无显著差异（P>0.05）,2组的DNA和RNA浓度显著高于Plank-Rychlo组（P<0.001）。结论：MORSE脱钙液在牙-颌骨-牙周联合组织处理方面具有综合性优势,值得在病理临床、教学及科研领域推广使用。

关键词: 牙-颌骨-牙周联合组织, 脱钙液, 染色效果, 核酸完整性

Abstract: PURPOSE: Comparative analysis of MORSE, formic acid and Plank-Rychlo decalcification solution for the treatment of combined tooth-mandibular-periodontal tissues to explore their application value. METHODS: Specimens including normal teeth, jaws and periodontal tissues discarded from Department of Oral Pathology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine were collected and randomly divided into 3 groups to analyse the differences in decalcification time, staining effect and nucleic acid integrity of 3 different decalcification solutions. SPSS 23.0 software package was used for statistical analysis. RESULTS: The mean decalcification time was 4.5, 3.5 and 3.6 days in the MORSE, formic acid and Plank-Rychlo group, respectively. H-E staining scores were in descending order in the MORSE, formic acid and Plank-Rychlo group. Immunohistochemical staining showed that AE1/AE3, p63 and Ki67 scores in MORSE group were better than those in the formic acid group and the Plank-Rychlo group, and Vimentin staining indices showed no significant difference between the 3 groups. In the fluorescence in situ hybridisation results, normal fluorescence signals were detected in 15 cases in MORSE group, 1 case in formic acid group and 4 cases in Plank-Rychlo group. The mean DNA concentration was 1.987, 1.963 and 1.115 ng/μL in MORSE, formic acid and Plank-Rychlo group, respectively; and the mean RNA concentration was 13.03, 11.08 and 1.66 ng/μL, respectively. There was no significant difference in DNA concentration and RNA concentration between MORSE and formic acid group(P>0.05), and both DNA and RNA concentrations were higher in the 2 groups than those in Plank-Rychlo group(P<0.001). CONCLUSIONS: MORSE decalcification solution has comprehensive advantages in the treatment of combined tooth-mandibular-periodontal tissues and is of value in the clinical, teaching and research aspects of pathology.

Key words: Combined tooth-mandibular-periodontal tissues, Decalcifying solution, Staining effect, Nucleic acid integrity

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