PTPRZ1在口腔黏膜下纤维性变癌变中的表达及临床意义

doi:10.19439/j.sjos.2017.02.015

上海口腔医学 ›› 2017, Vol. 26 ›› Issue (2): 198-203.doi: 10.19439/j.sjos.2017.02.015

PTPRZ1在口腔黏膜下纤维性变癌变中的表达及临床意义

朱蓉¹, 翦新春^1,2, 刘德裕¹, 周超², 王媛¹

1.中南大学湘雅医学院附属海口医院口腔颌面外科,海南海口 570208;
2.中南大学湘雅医院口腔颌面外科,湖南长沙 410008

收稿日期:2016-06-12 修回日期:2016-09-18 出版日期:2017-04-25 发布日期:2017-05-04
通讯作者: 翦新春,E-mail:jianxinchun@hotmail.com
作者简介:朱蓉（1989-）,女,硕士,住院医师,E-mail:752904290@qq.com
基金资助:
国家自然科学基金(81260166）

Expression of PTPRZ1 in oral squamous cell carcinoma originated from oral submucous fibrosis and its clinical significance

ZHU Rong¹, JIAN Xin-chun^1,2, LIU De-yu¹, ZHOU Chao², WANG Yuan¹

1.Department of Oral and Maxillofacial Surgery, Affiliated Haikou Hospital of Xiangya Medical College. Haikou 570208, Hainan Province;
2. Department of Oral and Maxillofacial Surgery, Xiangya Hospital, Central South University. Changsha 410008, Hunan Province, China

Received:2016-06-12 Revised:2016-09-18 Online:2017-04-25 Published:2017-05-04

摘要/Abstract

摘要： 目的: 研究蛋白酪氨酸磷酸酶Z1型受体(PTPRZ1)蛋白在口腔黏膜下纤维性病变（OSF）癌变中的表达及分布特征,探讨其在OSF癌变发生、发展过程中的作用及临床意义。方法: 利用免疫组织化学（immunohistochemistry,IHC）及蛋白印迹技术（Western blot, WB）检测36例OSF癌变患者、23例非OSF癌变鳞癌患者及21例身体健康者颊黏膜组织中PTPRZ1蛋白分布及表达差异。采用SPSS 21.0软件包对数据进行统计学分析。结果: 免疫组织化学果显示,PTPRZ1蛋白在OSF癌变组的颊黏膜中呈阳性表达,阳性表达率为72.22%;在非OSF癌变的颊黏膜鳞癌组织中呈弱阳性表达,表达率为43.47%;在正常颊黏膜组织中呈阴性表达。PTPRZ1蛋白在OSF癌变组及非OSF癌变组中的表达显著高于正常对照组（P<0.01）;OSF癌变组显著高于非OSF癌变的口腔癌组（P<0.05）。Western 印迹结果显示,PTPRZ1在正常组织中不表达,在非OSF癌变的口腔鳞癌中微量表达,在OSF癌变组中显著表达。PTPRZ1与一般临床资料相关性分析发现,PTPRZ1与复发及转移呈显著正相关性（r_k=0.642,P<0.01;r_k=0.656,P<0.01）。结论: PTPRZ1在OSF癌变的发生、发展及侵袭、转移过程中发挥重要作用,可作为早期诊断分子标志物及治疗的靶基因。

关键词: 酪氨酸蛋白激酶Z1型受体, 口腔黏膜下纤维性变, 口腔鳞癌

Abstract: PURPOSES: This study was aimed to detect the expression of PTPRZ1 in oral squamous cell carcinoma (OSCC) originated from oral submucous fibrosis (OSF), and discuss its role in the development and progression of OSCC originating from OSF as well as its clinical significance. METHODS: Immunohistochemisty (IHC) and Western blot (WB) for the expression and distribution of PTPRZ1 were carried out in 36 cases of OSF transforming into OSCC, 23 cases of OSCC and 21 cases of healthy controls. The data were analyzed by Chi-square test using SPSS 21.0 software package. RESULTS: IHC results demonstrated that the expression of PTPRZ1 in OSCC originated from the OSF was strongly positive and the rate of positive expression was 72.22%; expression of PTPRZ1 in OSCC was weakly positive, the positive rate was 43.47%; and the expression of PTPRZ1 in healthy controls was negative. The rate of PTPRZ1 positive expression was significantly higher in OSCC originated from OSF and OSCC with non-OSF than that of the healthy controls (P<0.01). The positive expression rate of OSCC originated from OSF was significantly higher than that of OSCC with non-OSF (P<0.05).WB results showed the expression of PTPRZ1 was weak in the healthy controls and in OSCC with non-OSF, but strong in OSCC originated from OSF. Correlation analysis between PTPRZ1 and clinical data showed PTPRZ1 was correlated with recurrence and metastasis positively (r_k=0.642, P<0.05; r_k =0.656, P<0.05). CONCLUSIONS: Differential expression of PTPRZ1 in OSCC originating from OSF and OSCC with non-OSF was significant. PTPRZ1, which plays an important role in the processes of development, invasion and metastasis of OSCC originated from OSF, can be used as a molecular marker for early diagnosis and targeted gene for treatment of OSCC originating from OSF.

Key words: Protein tyrosine phosphatase receptor type Z1, Oral submucous fibrosis, Oral squamous cell carcinoma

中图分类号:

R739.8

朱蓉, 翦新春, 刘德裕, 周超, 王媛. PTPRZ1在口腔黏膜下纤维性变癌变中的表达及临床意义[J]. 上海口腔医学, 2017, 26(2): 198-203.

ZHU Rong, JIAN Xin-chun, LIU De-yu, ZHOU Chao, WANG Yuan. Expression of PTPRZ1 in oral squamous cell carcinoma originated from oral submucous fibrosis and its clinical significance[J]. Shanghai Journal of Stomatology, 2017, 26(2): 198-203.

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PTPRZ1在口腔黏膜下纤维性变癌变中的表达及临床意义

Expression of PTPRZ1 in oral squamous cell carcinoma originated from oral submucous fibrosis and its clinical significance

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