Effects of sevoflurane on the migration of vascular endothelial cells

doi:10.19439/j.sjos.2017.03.008

Abstract

Abstract: PURPOSE: To evaluate the effects of sevoflurane on cell migration and expression of VE-cadherin mRNA in human umbilical vein endothelial cells(HUVECs). METHODS: HUVECs were allocated to 4 groups randomly (each group contained 4 culture dishes with a diameter of 10 cm), and were exposed to 2% sevoflurane (refer to 1.6 MAC) respectively for 0 h (control group which exposed to the same environment as the other 3 groups but without sevoflurane), 0.5 h, 1 h, 2 h. After sevoflurane exposure, HUVECs were subjected to scratch assay to observe the migration distance; RT-PCR was used to detect the expression of vascular endothelial-cadherin mRNA(VE-cadherin mRNA). Statistical analysis of the data was conducted using SPSS 13.0 software package. RESULTS: Scratch assay results suggested that the migration ability of HUVECs exposed to 2% sevoflurane for 2 h were significantly inhibited during 12 h after scratch compared with the control group(P<0.01), and RT-PCR results suggested that HUVECs exposed to 2% sevoflurane for 2 h expressed greater VE-cadherin mRNA than the control group(P<0.05). CONCLUSIONS: Sevoflurane exposure for 2 h can inhibit HUVECs migration and the mechanism may be up-regulating VE-cadherin expression.

Key words: Sevoflurane, Migration, HUVECs, VE-cadherin

CLC Number:

R614.2

SUN Yuan-qing, LIANG Bing, ZHANG Lei, XU Hui. Effects of sevoflurane on the migration of vascular endothelial cells[J]. Shanghai Journal of Stomatology, 2017, 26(3): 272-276.

References

[1] Sakai EM, Connolly LA, Klauck JA. Inhalation anesthesiology and volatile liquid anesthetics: focus on isoflurane, desflurane, and sevoflurane [J]. Pharmacotherapy, 2005, 25(12): 1773-1788.
[2] Vestweber D, Winderlich M, Cagna G, et al. Cell adhesion dynamics at endothelial junctions: VE-cadherin as a major player [J]. Trends Cell Biol, 2008, 19(1): 8-15.
[3] Boda-Heggemann J, Régnier-Vigouroux A, Franke WW. Beyond vessels: occurrence and regional clustering of vascular endothelial (VE-) cadherin-containing junctions in non-endothelial cells [J]. Cell Tissue Res, 2009, 335(1): 49-65.
[4] 朱萍, 韩正祥. VE-cadherin在调控血管内皮功能的研究进展 [J]. 现代肿瘤医学, 2011, 19(8): 1690-1694.
[5] Zhang L, Zhang J, Yang L, et al. Isoflurane and sevoflurane increase interleukin-6 levels through the nuclear factor-kappa B pathway in neuroglioma cells[J]. Br J Anaesth, 2013, 110(Suppl 1): i82-i91.
[6] Vestweber D. VE-cadherin: the major endothelial adhesion molecule controlling cellular junctions and blood vessel formation [J]. Arterioscler Thromb Vasc Biol, 2008, 28(2): 223-232.
[7] Xu K, Shuai Q, Li X, et al. Human VE-cadherin fusion protein as an artificial extracellular matrix enhancing the proliferation and differentiation functions of endothelial cell [J]. Biomacromolecules, 2016, 17(3): 756-766.
[8] Breier GF, Breviario L, Caveda R, et al. Molecular cloning and expression of murine VE-cadherin in early developing cardiovascular system [J]. Blood, 1996, 87(2): 630-641.
[9] Cai J, Jiang WG, Mansel RE. Inhibition of the expression of VE-cadherin /catenin complex by gamma linolenic acid in human vascular endothelial cells, and its impact on angiogenesis [J]. Biochem Biophys Res Commun, 1999, 258(1): 113-118.
[10] Nakhuda GS, Zimmermann RC, Bohlen P, et al. Inhibition of the vascular endothelial cell (VE) -specific adhesion molecule VE-cadherin blocks gonadotropin-dependent folliculogenesis and corpus luteum formation and angiogenesis [J]. Endocrinology, 2005, 146(3): 1053-1059.
[11] Caveda L, Martin-Padura I, Navarro P, et al. Inhibition of cultured cell growth by vascular endothelial cadherin (cadherin-5/VE-cadherin) [J]. J Clin Invest, 1996, 98(4): 886-893.
[12] Shrivastava-Ranjan P, Rollin PE, Spiropoulou CF. Andes virus disrupts the endothelial cell barrier by induction of vascular endothelial growth factor and downregulation of VE-cadherin [J]. J Virol, 2010, 84(21): 11227-11234.
[13] Miyazaki T, Taketomi Y, Takemoto M, et al. m-Calpain induction in vascular endothelial cells on human an mouse atheromas and its roles in VE-cadherin disorganization and atherosclerosis [J]. Circulation, 2011, 124(23): 2522-2532.
[14] Miao H, Hu YL, Shiu YT, et al. Effects of flow patterns on the localization and expression of VE-cadherin at vascular endothelial cell functions: in vivo and in vitro investigations [J]. J Vasc Res, 2005, 42(1): 77-89.
[15] Dejana E. Endothelial adherens junctions: implications in the control of vascular permeability and angiogenesis [J]. J Clin Invest, 1996, 98(9): 1949-1953.
[16] Ergönenc J, Ergönenc T, Īdin K, et al. The recovery time of sevoflurane and desflurane and the effects of anesthesia on mental and psychomotor functions and pain [J]. Anesth Essays Res, 2014, 8(3): 367-371.
[17] Li J, Yuan T, Zhao X, et al. Protective effects of sevoflurane in hepatic ischemia-reperfusion injury[J]. Int Immunopathol Pharmacol, 2016, 29(2): 300-307.
[18] Chappell D, Heindl B, Jacob M, et al. Sevoflurane reduces leukocyte and platelet adhesion after ischemia-reperfusion by protecting the endothelial glycocalyx [J]. Anesthesiology, 2011, 115(3): 483-491.
[19] Annecke T, Chappell D, Chen C, et al. Sevoflurane preserves the endothelial glycocalyx against ischemia-reperfusion injury [J]. Br J Anaesth, 2010, 104(4): 414-421.
[20] Lindholm EE, Aune E, Norén CB, et al. The anesthesia in abdominal aortic surgery (ABSENT) study: a prospective, randomized, controlled trial comparing troponin T release with fentanyl-sevofluran and propofol-remifentanil anesthesia in major vascular surgery [J]. Anesthesiology, 2013, 119(4): 802-812.
[21] Lindholm EE, Aune E, Seljeflot I, et al. Biomakers of inflammation in major vascular surgery: a prospective randomised trial [J]. Acta Anaesthesiol Scand, 2015, 59(6): 773-787.
[22] Acharya NK, Goldwaser EL, Forsberg MM, et al. Sevoflurane and isoflurane induce structural changes in brain vascular endothelial cells and increase blood-brain barrier permeability: possible link to postoperative delirium and cognitive decline [J]. Brain Res, 2015, 1620: 29-41.
[23] Huang Y, Tan Q, Chen R, et al. Sevoflurane prevents lipopolysaccharide-induced barrier dysfunction in human lung microvascular endothelial cells: Rho-mediated alterations of VE-cadherin [J]. Biochem Biophys Res Commun, 2015, 468(1-2): 119-124.