Expression and clinical significance of PUMA gene in salivary adenoid cystic carcinoma

Shanghai Journal of Stomatology ›› 2016, Vol. 25 ›› Issue (3): 314-316.

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Expression and clinical significance of PUMA gene in salivary adenoid cystic carcinoma

LEI Yin-tao¹, LI Jun-fu¹, JIANG Juan¹, LI Xiao-guang², WANG Xu-xia³, CHEN Dai-yun¹

1. Department of Stomatology, Affiliated Hospital of Taishan Medical University. Tai'an 271000;
2. Department of Stomatology, Tai'an City Central Hospital. Tai'an 271000;
3. School of Stomatology Shandong University. Jinan 250012, Shandong Province, China

Received:2015-08-07 Revised:2015-11-06 Online:2016-06-25 Published:2016-07-22

Abstract

Abstract: PURPOSE: To explore the expression of p53 up-regulated modulator of apoptosis (PUMA) gene in salivary adenoid cystic carcinoma (SACC) and its clinical significance. METHODS: The expressions of PUMA gene and protein were detected in 27 SACC tumor-adjacent tissues (group A) and 27 SACC tumor tissues (group B) with real-time quantitative PCR and Western blot. Statistical analysis was performed using SPSS 19.0 software package. RESULTS: The expression of PUMA gene in groups A as calibrator was 1,and expression of PUMA gene in group B was 0.57±0.17. The expression of PUMA protein in groups A and B were 0.94±0.12 and 0.36±0.12,respectively. The expressions of PUMA gene and protein were significantly lower in group B than in group A (P<0.05). CONCLUSIONS: The expression of puma gene in SACC tissues is down-regulated, which is negatively correlated with the development of SACC.

Key words: p53 up-regulated modulator of apoptosis, Salivary adenoid cystic carcinoma, Real-time quantitative PCR

CLC Number:

R739.8

LEI Yin-tao, LI Jun-fu, JIANG Juan, LI Xiao-guang, WANG Xu-xia, CHEN Dai-yun. Expression and clinical significance of PUMA gene in salivary adenoid cystic carcinoma[J]. Shanghai Journal of Stomatology, 2016, 25(3): 314-316.

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Expression and clinical significance of PUMA gene in salivary adenoid cystic carcinoma

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