Osteogenic gene expression of human periodontal ligament stem cells during osteogenic induction

Shanghai Journal of Stomatology ›› 2014, Vol. 23 ›› Issue (4): 391-396.

• Basic Study • Previous Articles Next Articles

Osteogenic gene expression of human periodontal ligament stem cells during osteogenic induction

ZHAO Jing-lei, JIANG ling-yong, MAO Li-xia, LIU Jia-qiang, FANG Bing

Department of Oral and Cranomaxillofacial Science, Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China

Received:2013-10-25 Online:2014-08-20 Published:2014-10-20
Supported by:
Supported by National Natural Science Foundation of China (81371178), Key Basic Research Foundation of Science and Technology Committee of Shanghai Municipality (12JC1405700), Collaborative Foundation of Medical and Engineering Science of Shanghai Jiao Tong University (YG2012MS40), Key Basic Research Foundation of Science and Technology Committee of Shanghai Municipality (12JC1405700), "Chen Xing" Project from Shanghai Jiao Tong University, and Innovative Research Team of Shanghai Municipal Education Commission.

Abstract

Abstract: PURPOSE: To isolate and identify the human periodontal ligament stem cells, evaluate osteogenetic capacity, and investigate the changes of osteogenic bone related gene expression in mineralized medium at different times. METHODS: PDLSCs were isolated by tissue culture and magnetic activated cell sorting. Immunofluorescence staining was used for identification. The general situation of osteogenesis was assessed with alkaline phosphatase (ALP) and alizarin red staining. Real-time PCR was used to detect the expression of genes in osteoinduction. SPSS12.0 software package was used for data analysis. RESULTS: Tissue culture with magnetic cell sorting could isolate high-purity human periodontal ligament stem cells. During the osteogenic process, the expression of FoxO1 and Runx2 firstly increased and then decreased, ALP and OCN gene levels continued to increase. CONCLUSIONS: Similar to osteogenesis, ALP, Runx2, FoxO1 and OCN are regularly expressed during osteogenic induction.

Key words: Periodontal ligament stem cell, Osteogenic induction, Osteogenic differentiation

CLC Number:

R781.4

ZHAO Jing-lei, JIANG ling-yong, MAO Li-xia, LIU Jia-qiang, FANG Bing. Osteogenic gene expression of human periodontal ligament stem cells during osteogenic induction[J]. Shanghai Journal of Stomatology, 2014, 23(4): 391-396.

References

[1] Shimono M, Ishikawa T, Ishikawa H, et al. Regulatory mechanisms of periodontal regeneration [J]. Microsc Res Tech, 2003, 60(5): 491-502.
[2] Seo BM, Miura M, Gronthos S, et al. Investigation of multipotent postnatal stem cells from human periodontal ligament [J]. Lancet, 2004, 364(9429): 149-155.
[3] Ge S, Zhao N, Wang L, et al. Bone repair by periodontal ligament stem cell seeded nanohydroxyapatite-chitosan scaffold [J]. Int J Nanomedicine, 2012, 7: 5405-5414.
[4] 高秦, 刘宏伟, 金岩, 等. 人牙周膜干细胞的体外分离、纯化及初步鉴定 [J].实用口腔医学杂志, 2006, 22(1): 34-37.
[5] Fortino VR, Chen RS, Pelaez D, et al. Neurogenesis of neural crest derived periodontal ligament stem cells by EGF and bFGF [J]. J Cell Physiol, 2013 Sep 16. [Epub ahead of print]
[6] Gronthos S, Mrozik K, Shi S, et al. Ovine periodontal ligament stem cells: isolation, characterization, and differentiation potential [J]. Calcif Tissue Int, 2006, 79(5): 310-317.
[7] Tsai MT, Li WJ, Tuan RS, et al. Modulation of osteogenesis in human mesenchymal stem cells by specific pulsed electromagnetic field stimulation [J]. J Orthop Res, 2009, 27(9): 1169-1174.
[8] Wada Y, Kataoka H, Yokose S, et a1. Changes in osteoblast phenotype during differentiation of enzymatically isolated rat calvaria cells [J]. Bone, 1998, 22(5): 479-485.
[9] Komori T. Regulation of bone development and extracellular matrix protein genes by RUNX2[J]. Cell Tissue Res,2010,339(1): 189-195.
[10] Teixeira CC, Liu Y, Thant LM, et al. Foxo1, a novel regulator of osteoblast differentiation and skeletogenesis [J]. J Biol Chem, 2010, 285(40): 31055-31065.
[11] Siqueira MF, Flowers S, Bhattacharya R, et al. FOXO1 modulates osteoblast differentiation [J]. Bone, 2011, 48(5): 1043-1051.
[12] Hauschka PV, Lian JB, Cole DE, et al. Osteocalcin and matrix Gla protein: vitamin K-dependent proteins in bone [J]. Physiol Rev, 1989, 69(3): 990-1047.

[1]	SHEN Yi-fen, LIU Chao, TANG Ying, YANG Tao, GU Yong-chun. The role of ROS/JNK/caspase 3 axis in apoptosis induction by menthol-favored electronic cigarette liquid in human periodontal ligament stem cells [J]. Shanghai Journal of Stomatology, 2024, 33(1): 40-48.
[2]	LI Li, LIAN Wen-Wei. BMP9 promotes osteogenic differentiation of periodontal ligament stem cells under inflammatory environment via ERK5/KLF4 signaling pathway [J]. Shanghai Journal of Stomatology, 2023, 32(6): 583-589.
[3]	Dilidaer·Taxifulati, NIU Ya-qi, Reyisha·Abudukeyimu, WANG Lin. Effect of lncRNA AWPPH on human periodontal ligament cell proliferation and osteogenic differentiation by regulating Notch signaling pathway [J]. Shanghai Journal of Stomatology, 2023, 32(1): 23-27.
[4]	HU Yi-peng, OU Xiao-yan, ZHONG Hong-mei. Effect, mechanism of miR-124on osteogenic differentiation of dental pulp mesenchymal stem cells [J]. Shanghai Journal of Stomatology, 2022, 31(5): 466-470.
[5]	DUAN Xin, LIU Fei, LIU Ke-ke, WANG Song-song, ZHANG Yun-tao. Effect of dual sustained-release nanofiber scaffolds embedded with BMP-2protein nanospheres on the proliferation, differentiation of MC3T3-E1cells [J]. Shanghai Journal of Stomatology, 2022, 31(5): 476-482.
[6]	DONG Jia-chen, SHU Rong. The effect of inflammation on proliferation and osteogenic differentiation of periodontal ligament cells [J]. Shanghai Journal of Stomatology, 2022, 31(3): 243-247.
[7]	LIN Wei-long, WU Xiao-pei, WANG Xiao-ming, HE Wei-wei. Effect of miR-199a regulating IGF1 expression on differentiation of osteoblasts under mechanical stimulation [J]. Shanghai Journal of Stomatology, 2022, 31(2): 132-137.
[8]	TANG Ying, SHEN Yi-fen, LIU Chao, QIU Yin-feng, GU Yong-chun, YU Jin-hua. Effect of high-concentration fluoride on apoptosis of human periodontal ligament stem cells [J]. Shanghai Journal of Stomatology, 2021, 30(4): 360-366.
[9]	MA Chun-yan, PAN Qing, HE Lei, YANG Hai-bing. Effects of extracellular matrix PDMS stiffness on proliferation and osteogenic differentiation of dental pulp stem cells [J]. Shanghai Journal of Stomatology, 2021, 30(3): 253-257.
[10]	ZHU Chen-yuan, XU Ling, YU Wei-qiang. Berberine promotes osteogenic differentiation of rat adipose-derived stem cells through JNK signaling pathway [J]. Shanghai Journal of Stomatology, 2021, 30(3): 258-262.
[11]	WANG Xu-dong, ZHANG Cheng-yao, ZHANG Shi-jian, SHI Jing-cun, WANG Lei. Simultaneous innervation revitalizes bone marrow mesenchymal stem cells from postoperative iliac grafts [J]. Shanghai Journal of Stomatology, 2020, 29(6): 561-566.
[12]	ZHENG Jing-jing, KANG Xin-yun, LI Shao-ming, LIU Jia-cheng, SUN Kai, GAO Ling, HOU Tie-zhou. Effects of MTA, iRoot SP and AH Plus on proliferation and differentiation of human periodontal ligament stem cells [J]. Shanghai Journal of Stomatology, 2020, 29(5): 449-455.
[13]	CHANG Si-jia, YANG Liu, LI Ran. Osteogenic stimulation of human dental pulp stem cells with gelatin-hydroxyapatite-tricalcium phosphate scaffold [J]. Shanghai Journal of Stomatology, 2020, 29(5): 492-498.
[14]	LI Wen-jing, CHEN Hui-zhen, LIU Cong-na, ZAHNG Zhi-yong. Expression level and effect of P2X7 receptor mRNA during osteogenic differentiation of human periodontal ligament stem cells [J]. Shanghai Journal of Stomatology, 2020, 29(4): 365-369.
[15]	DENG Jia-jia, PAN Jie, YU Li-ming, ZHANG Wei-hua, ZHU Lu-ying, HAN Xin-xin, LIU Yue-hua. Biological characteristics of periodontal ligament stem cells modified by platelet derived growth factor BB [J]. Shanghai Journal of Stomatology, 2020, 29(4): 343-349.

Osteogenic gene expression of human periodontal ligament stem cells during osteogenic induction

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments