Effects of laser combined with periodontal basic treatment on periodontal indices, subgingival microflora, adiponectin, MMP-13 and IL-1β in gingival crevicular fluid in patients with periodontitis

doi:10.19439/j.sjos.2024.03.015

Abstract

Abstract: PURPOSE: To investigate the effects of laser combined with periodontal basic treatment on periodontal indices, subgingival flora, adiponectin, matrix metalloproteinase-13 (MMP-13) and interleukin-1β (IL-1β) in patients with periodontitis. METHODS: A retrospective analysis was performed on 100 patients with periodontitis diagnosed and treated in Hengshui People's Hospital from December 2022 to July 2023. According to treatment methods, the patients were divided into control group (n=51) and experimental group (n=49). The control group received periodontal basic treatment, and the experimental group received laser treatment on the basis of the control group. The periodontal indexes, subgingival microflora, adiponectin, MMP-13, IL-1β and bone metabolic factors of gingival crevicular fluid before and after treatment were compared between the two groups, as well as the clinical therapeutic effect. Statistical analysis was performed with SPSS 22.0 software package. RESULTS: After treatment, probing depth(PD), bleeding on probing(BOP), gingival index(GI) and plaque index (PLI) in the experimental group were lower than before treatment (P<0.05), PD, BOP and PLI in the control group were lower than before treatment (P<0.05), and PD, BOP, GI and PLI in the experimental group were significantly lower than those in control group (P<0.05). After treatment, Lactobacillus, Clostridium and Bacteroides in both groups were significantly lower than before treatment (P<0.05), and the experimental group was significantly lower than the control group(P<0.05). After treatment, adiponectin in gingival crevicular fluid increased in both groups compared with before treatment(P<0.05), and MMP-13 and IL-1β in gingival crevicular fluid decreased in both groups compared with before treatment (P<0.05), and adiponectin in gingival crevicular fluid in the experimental group was significantly higher than that in the control group (P<0.05), MMP-13 and IL-1β in the experimental group were significantly higher than that in the control group (P<0.05). After treatment, procollagenⅠtype N-terminal peptide (PINP), cross linked C-telopeptide of type Ⅰ collagen(CXT) and bone glaprotein (BGP) were significantly higher than those before treatment (P<0.05), and the experimental group was significantly higher than the control group (P<0.05). The total effective rate of the experimental group was significantly higher than that of the control group (P<0.05). CONCLUSIONS: Laser combined with periodontal basic treatment can effectively improve periodontal indexes, reduce subgingival flora, increase the levels of adiponectin and bone metabolic factor in gingival crevicular fluid, reduce the levels of MMP-13 and IL-1β in gingival crevicular fluid, and improve the clinical therapeutic effect in patients with periodontitis.

Key words: Laser, Periodontal basic treatment, Periodontitis, Subgingival flora, Adiponectin, Matrix metalloproteinase 13, Interleukin 1β

CLC Number:

R781.4

ZHANG Zhong-yue, CHEN Li, YAN Na, ZHANG Shu-hua. Effects of laser combined with periodontal basic treatment on periodontal indices, subgingival microflora, adiponectin, MMP-13 and IL-1β in gingival crevicular fluid in patients with periodontitis[J]. Shanghai Journal of Stomatology, 2024, 33(3): 295-300.

References

[1] Cosgarea R, Jepsen S, Heumann C, et al.Clinical, microbiological, and immunological effects of 3- or 7-day systemic antibiotics adjunctive to subgingival instrumentation in patients with aggressive(Stage Ⅲ/Ⅳ Grade C) periodontitis: a randomized placebo-controlled clinical trial[J]. J Clin Periodontol, 2022, 49(11): 1106-1120.
[2] Rapone B, Ferrara E, Corsalini M, et al.Inflammatory status and glycemic control level of patients with type 2 diabetes and periodontitis: a randomized clinical trial[J]. Int J Environ Res Public Health, 2021, 18(6): 3018-3033.
[3] Bartold PM.Lifestyle and periodontitis: The emergence of personalized periodontics[J]. Periodontol 2000, 2018, 78(1):7-11.
[4] Lorenzo-Erro SM, Andrade E, Massa F, et al.Periodontitis prevalence and associated factors: a comparison of two examination protocols[J]. Acta Odontol Latinoam, 2022,35(3):178-187.
[5] Yan Y, Zhan Y, Wang X, et al.Clinical evaluation of ultrasonic subgingival debridement versus ultrasonic subgingival scaling combined with manual root planing in the treatment of periodontitis: study protocol for a randomized controlled trial[J]. Trials, 2020, 21(1):113.
[6] 程哲贤, 周璿. 激光在牙周炎非手术治疗中的应用[J]. 中华老年医学杂志, 2021, 40(12): 1600-1602.
[7] 吴昱卓, 周艳, 梅幼敏, 等. Er:YAG激光治疗Ⅱ度根分叉病变牙周炎患者的临床疗效评价[J]. 上海口腔医学, 2020, 29(3): 308-311.
[8] Chapple ILC, Mealey BL, Van Dyke TE, et al.Periodontal health and gingival disceases and conditions on an intact and a reduced periodontium: consensus report of workgroup 1 of the 2017 World Workshop on the Classification of Periodontal and Peri-implant Diseases and Conditions[J]. J Clin Periodontol, 2018, 45(Suppl 20): S68-S77.
[9] Kwon T, Lamster IB, Levin L.Current concepts in the management of periodontitis[J]. Int Dent J, 2021, 71(6):462-476.
[10] Sánchez-Medrano AG, Martinez-Martinez RE, Soria-Guerra R, et al.A systematic review of the protein composition of whole saliva in subjects with healthy periodontium compared with chronic periodontitis[J]. PLoS One, 2023,18(5):e0286079.
[11] Jiang Y, Feng J, Du J, et al.Clinical and biochemical effect of laser as an adjunct to non-surgical treatment of chronic periodontitis[J]. Oral Dis, 2022,28(4):1042-1057.
[12] 黄辛兆, 罗舒心, 黄铮, 等. Nd:YAP激光辅助牙周基础治疗对Ⅲ期B/C级牙周炎患者龈沟液中HIF-1α、ICAM-1水平的影响[J]. 实用口腔医学杂志, 2023, 39(1): 103-106.
[13] 朱晓娟, 代海涛, 李艳, 等. 葡萄籽原花青素对糖尿病牙周炎大鼠血清脂联素和牙周组织炎性状态的影响[J]. 医学研究生学报, 2022, 35(9): 903-908.
[14] 杨琦, 穆森, 詹渊博, 等. 牙周基础治疗对慢性牙周炎患者非刺激性全唾液、龈沟液及血清中基质金属蛋白酶13水平的影响[J]. 实用口腔医学杂志, 2020, 36(2): 381-384.
[15] Wang RP, Huang J, Chan KWY, et al.IL-1β and TNF-α play an important role in modulating the risk of periodontitis and Alzheimer's disease[J]. J Neuroinflammation, 2023, 20(1):71-80.
[16] 谢芬, 庞红霞, 吴云菲, 等. 血清IL-1β及尿DPd与老年2型糖尿病合并牙周炎患者牙周学指标的相关性[J]. 中国老年学杂志, 2021, 41(21): 4737-4741.
[17] Gillett MJ, Vasikaran SD, Inderjeeth CA.The role of PINP in diagnosis and management of metabolic bone disease[J]. Clin Biochem Rev, 2021, 42(1):3-10.
[18] 范晓琳, 杨小华, 刘月军, 等. 2型糖尿病性骨质疏松患者骨小梁分数与骨代谢标志物的相关性分析[J]. 中国骨质疏松杂志, 2021, 27(11): 1599-1602,1613.