Preparation and identification of polyclonal antibody of Streptococcus salivarius 57.I urease

Shanghai Journal of Stomatology ›› 2016, Vol. 25 ›› Issue (6): 678-681.

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Preparation and identification of polyclonal antibody of Streptococcus salivarius 57.I urease

WANG Yan¹, TAO Dan-ying², FENG Xi-ping²

1.Department of Preventive Dentistry, Shanghai Stomatological Hospital. Shanghai 200001;
2.Department of Preventive Dentistry, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China

Received:2016-05-11 Online:2016-12-25 Published:2016-12-29

Abstract

Abstract: PURPOSE: To prepare and identify polyclonal antibody of Streptococcus salivarius 57.I urease. METHODS: The biggest structural subunit of Streptococcus salivarius 57.I urease, UreC, was obtained by gene clone, IPTG-induced expression, and purification through affinity chromatography. Anti-sera and polyclonal antibody were raised by immunizing rabbits with purified UreC. Western blot was utilized to detect the specific combination of polyclonal antibody with UreC. RESULTS: Purified UreC protein was prepared and used as antigen to immunize rabbits. Polyclonal antibody was obtained, and Western blot displayed a specific band of the polyclonal antibody with UreC about 62 kD as anticipated. CONCLUSIONS: Polyclonal antibody against Streptococcus salivarius 57.I urease is obtained, which provides an important tool to explore the function of urease and its relationship with dental caries.

Key words: Streptococcus salivarius, Urease, Antibody

CLC Number:

R780.2

WANG Yan, TAO Dan-ying, FENG Xi-ping. Preparation and identification of polyclonal antibody of Streptococcus salivarius 57.I urease[J]. Shanghai Journal of Stomatology, 2016, 25(6): 678-681.

References

[1] Chen YY, Betzenhauser MJ, Burne RA. cis-Acting elements that regulate the low-pH-inducible urease operon of Streptococcus salivarius [J]. Microbiology, 2002, 148(Pt 11): 3599-3608.
[2] Gordan VV, Garvan CW, Ottenga ME, et al. Could alkali production be considered an approach for caries control?[J]. Caries Res, 2010, 44(6): 547-554.
[3] Nascimento MM, Gordan VV, Garvan CW. Correlations of oral bacterial arginine and urea catabolism with caries experience [J]. Oral Microbiol Immunol, 2009, 24(2): 89-95.
[4] Chen YY, Burne RA. Identification and characterization of the nickel uptake system for urease biogenesis in Streptococcus salivarius 57.Ⅰ[J]. J Bacteriol, 2003, 185(23): 6773-6779.
[5] Huang SC, Burne RA, Chen YY. The pH-dependent expression of the urease operon in Streptococcus salivarius is mediated by CodY [J]. Appl Environ Microbiol, 2014, 80(17): 5386-5393.
[6] 王艳, 冯希平, 谢幼华, 等. 唾液链球菌尿素酶基因的克隆和表达 [J]. 中华口腔医学杂志, 2010, 45(8): 783-785.
[7] Morou-Bermudez E, Rodriguez S, Bello AS, et al. Urease and dental plaque microbial profiles in children [J]. PLoS One, 2015, 10(9):e0139315.
[8] Lee SH, Park JM, Han YM, et al. Unpleasant journey from helicobacter pylori-associated gastritis to gastric cancer: cancer prevention by taking a detour[J]. Korean J Gastroenterol, 2015, 66(6): 303-311.
[9] Ahn HJ, Lee DS. Helicobacter pylori in gastric carcinogenesis[J]. World J Gastrointest Oncol, 2015, 7(12): 455-465.
[10] Liu YL, Nascimento M, Burne RA. Progress toward understanding the contribution of alkali generation in dental biofilms to inhibition of dental caries [J]. Int J Oral Sci, 2012, 4(3):135-140.
[11] Gordan VV, McEdward DL, Ottenga ME, et al. Alkali production in the mouth and its relationship with certain patient's characteristics [J]. J Appl Oral Sci, 2014, 22(6): 560-568.
[12] Reyes E, Martin J, Moncada G, et al. Caries-free subjects have high levels of urease and arginine deiminase activity [J]. J Appl Oral Sci, 2014, 22(3): 235-240.
[13] Appelgren L, Dahlén A, Eriksson C, et al. Dental plaque pH and ureolytic activity in children and adults of a low caries population [J]. Acta Odontol Scand, 2014, 72(3): 194-201.
[14] Moncada G, Maureira J, Neira M, et al. Salivary urease and ADS enzymatic activity as endogenous protection against dental caries in children [J]. J Clin Pediatr Dent, 2015, 39(4): 358-363.
[15] Shu M, Morou-Bermudez E, Suárez-Pérez E, et al. The relationship between dental caries status and dental plaque urease activity [J]. Oral Microbiol Immunol, 2007, 22(1): 61-66.
[16] Morou-Bermudez E, Elias-Boneta A, Billings RJ, et al. Urease activity as a risk factor for caries development in children during a three-year study period: a survival analysis approach [J]. Arch Oral Biol, 2011, 56(12):1560-1568.
[17] Kishikawa H, Kimura K, Takarabe S, et al. Helicobacter pylori antibody titer and gastric cancer screening [J]. Dis Markers, 2015, 2015:156719.
[18] Fatema J, Khan AH, Uddin MJ, et al. Chronic gastritis and its association with H. Pylori infection [J]. Mymensingh Med J, 2015, 24(4): 717-722.

Preparation and identification of polyclonal antibody of Streptococcus salivarius 57.I urease

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