Shanghai Journal of Stomatology

Shanghai Journal of Stomatology ›› 2015, Vol. 24 ›› Issue (1): 46-51.

• Basic Study • Previous Articles     Next Articles

Study of relationship between (Ca+Zn)/P of pure titanium surface coating and biological activity of osteoblasts

SHANG Ya-wei1,ZHANG Wei2,HE Jing1,WANG Dan-ning1,ZHONG Ming3,ZHAO Bao-hong1   

  1. 1.Oral Implanting Center of Hospital of Stomatology, China Medical University; Oral Implanting Laboratory, Oral Medical Research Institute of Liaoning Province, Translational Medicine Research Center of Oral Disease of Liaoning Province. Shenyang 110002;
    2. Institute of Metal Corrosion and Protection, China Academy of Sciences. Shenyang 110016;
    3. Central Laboratory, School of Stomatology, China Medical University. Shenyang 110002, Liaoning Province, China
  • Received:2014-05-12 Online:2015-02-20 Published:2015-07-24
  • Supported by:
    Natural Science Foundation of Liaoning Province (20092093), Science and Technology Project of Liaoning Province (2013225049), and Science and Technology Project of Shenyang City (F11-264-1-25)

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Abstract: PURPOSE: To study osteoblast (MC3T3-E1 cells) adhesion, proliferation and differentiation activity on the surface of pure titanium with different (Ca+Zn)/P ratios,and determine Ca,P,Zn contents offering best biological activity on pure titanium surface. METHODS: In the micro-arc oxidation electrolyte, Ca, P with a certain concentration, and 5 different concentrations of Zn (0,0.01,0.03,0.04, and 0.06 mol/L) were added, which made the mole ratio of Ca and P element close to the ratio of hydroxyapatite. (Ca+Zn)/P ratio of bioactive coating on 5 different pure titanium material surfaces were prepared, which named S0 and S1, S2, S3 and S4 group, respectively. The surface elements, existence form, (Ca + Zn)/P ratio and surface morphology were analyzed by XPS and SEM; MC3T3-E1 cells adhesion, proliferation and differentiation cultured on the material surface were analyzed by SEM, MTT method and ALP activity assay. RESULTS: The data was analyzed with SPSS13.0 software package. With the increase of Zn content in the electrolyte, (Ca+Zn)/P ratios were 2.94, 2.64 2.71,2.87 and 2.72 in S0 ~ S4 group, respectively. (Ca+Zn)/P ratio was maximal in S3 group and decreased in S4 group. MC3T3-E1 cell adhesion, proliferation and differentiation capacity on material surface of 5 groups changed in the following order: S3 > S4 > S2 > S1 > S0, with significant differences among 5 groups (P<0.05). CONCLUSIONS: Coating with different concentrations of Ca, P, Zn on pure titanium surface by micro-arc oxidation technique, the adhesion, proliferation and differentiation ability of osteoblast increase as (Ca+Zn)/P ratio increase, and cell biological activity is the best when the (Ca+Zn)/P ratio reached 2.87.

Key words: Micro-arcoxidation, Titanium, Zinc, Calcium, Phosphorus, Osteoblast

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