Effect of high glucose on migration of BMSCs through inhibiting CXCR-4

Abstract

Abstract: PURPOSE: To explore the effect of high glucose on migration of BMSCs through inhibiting CXCR-4. METHODS: Bone marrow stromal cells (BMSCs) were obtained from the mandible of Wistar rats and stimulated with different concentrations of glucose (5.5, 16.5 mmol/L). The optimum concentration of SDF-1 was evaluated by Transwell assay in physiological glucose concentration (5.5 mmol/L). In the optimum concentration of SDF-1 condition, we detected the effect of SDF-1 and AMD3100 on migration of BMSCs in different concentrations of glucose (5.5, 16.5 mmol/L). CXCR-4 protein levels were determined by Western blot. The mRNA expression of CXCR-4 and MMP-2 were tested by RT-PCR. SPSS 11.0 software package was used for statistical analysis. RESULTS: The optimum concentration of SDF-1 was 100 ng/mL. High glucose could inhibit the migration of BMSCs. In different concentrations of glucose, SDF-1 could promote the migration of BMSCs, but AMD3100 could inhibit this promotion. High glucose condition could inhibit the secretion of CXCR-4 and mRNA expression of CXCR-4 and MMP-2. CONCLUSIONS: High glucose inhibits migration of BMSCs by inhibiting CXCR-4 through SDF-1/CXCR-4 pathway.

Key words: Supported by National Natural Science Foundation of China (81271180, 31200741).

CLC Number:

R708.2

ZHANG Bo, LIU Na, GU Bin, WU Hao, GAO Yu-xuan, WANG Dong-sheng, LIU Hong-chen. Effect of high glucose on migration of BMSCs through inhibiting CXCR-4[J]. Shanghai Journal of Stomatology, 2014, 23(6): 646-650.

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