Changes of the microarchitecture of alveolar bone due to different duration of ovariectomy: a Micro-CT study in rats

Shanghai Journal of Stomatology ›› 2014, Vol. 23 ›› Issue (6): 641-645.

• Basic Study • Next Articles

Changes of the microarchitecture of alveolar bone due to different duration of ovariectomy: a Micro-CT study in rats

DAI Qing-gang, FANG Bing, ZHANG Peng, YANG Xiao, WANG Jie, OUYANG Ning-juan, WU Yu-qiong, JIANG Ling-yong

Department of Oral and Craniomaxillofacial Science, Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology. Shanghai 200011, China

Received:2014-05-04 Revised:2014-07-09 Online:2014-12-20 Published:2015-01-08
Supported by:
National Natural Science Foundation of China (81371121, 11342005, 30901698, 10972142); Natural Science Foundation of Shanghai Municipality (13ZR1423700), Key Basic Research Foundation of Science and Technology Committee of Shanghai Municipality (12JC1405700); Collaborative Foundation of Medical and Engineering Science of Shanghai Jiao Tong University (YG2012MS40), “Chen Xing” Project from Shanghai Jiao Tong University and Innovative Research Team of Shanghai Municipal Education Commission

Abstract

Abstract: PURPOSE: To study the changes of microarchitecture of alveolar bone due to different duration of ovariectomy in rats. METHODS: Twenty-four virgin Sprague-Dawley rats were randomly assigned to ovariectomy group (OVX) or sham-ovariectomy group (sham). OVX rats were subjected to bilateral ovariectomy and sham-ovariectomy was conducted in sham rats. Six rats of each group were sacrificed respectively 3 months and 6 months after surgery. The right semi-maxilla of all rats were scanned by Micro-CT, and the inter-radicular alveolar bone of the maxillary first molar was analyzed. Statistical analysis was carried out with SPSS 16.0 software package. RESULTS: Two-dimensional and three-dimensional reconstructed images of the alveolar bone showed porotic changes in rats both 3 months and 6 months after ovariectomy, including thinner, looser trabeculae and expanded bone marrow. When compared with corresponding sham rats, BMD, BV/TV and Tb.Th of alveolar bone significantly decreased in OVX rats both 3 months and 6 months after ovariectomy (P<0.05). Tb.N and Tb.Sp significantly increased in both OVX groups (P<0.05). When compared with 3 months after ovariectomy, the rats 6 months after ovaroectomy shared deceased BMD, BV/TV and Tb.Th (P<0.05) and increased Tb.N (P<0.05). CONCLUSIONS: Bone loss and deterioration of trabeculae of alveolar bone aggravates with the extended duration of ovariectomy in OVX rats.

Key words: Ovariectomy, Alveolar bone, Osteoporosis, Microarchitecture, Micro-CT

CLC Number:

R782.1

DAI Qing-gang, FANG Bing, ZHANG Peng, YANG Xiao, WANG Jie, OUYANG Ning-juan, WU Yu-qiong, JIANG Ling-yong. Changes of the microarchitecture of alveolar bone due to different duration of ovariectomy: a Micro-CT study in rats[J]. Shanghai Journal of Stomatology, 2014, 23(6): 641-645.

References

[1] 皮昕. 口腔解剖生理学[M]. 第6版. 北京: 人民卫生出版社, 2006: 88-96.
[2] Mavropoulos A, Rizzoli R, Ammann P. Different responsiveness of alveolar and tibial bone to bone loss stimuli [J]. J Bone Miner Res, 2007, 22(3): 403-410.
[3] Leung FY, Rabie AB, Wong RW. Osteoporosis, osteonecrosis, and orthodontics [J]. World J Orthod, 2009, 10(3): 261-271.
[4] Dempster DW, Birchman R, Xu R, et al. Temporal changes in cancellous bone structure of rats immediately after ovariectomy [J]. Bone, 1995, 16(1): 157-161.
[5] Miller SC, Wronski TJ. Long-term osteopenic changes in cancellous bone structure in ovariectomized rats [J]. Anat Rec, 1993, 236(3): 433-441.
[6] Dai QG, Zhang P, Wu YQ, et al. Ovariectomy induces osteoporosis in the maxillary alveolar bone: an in vivo micro-CT and histomorphometric analysis in rats [J]. Oral Dis, 2014, 20(5): 514-520.
[7] Wronski TJ, Cintrón M, Dann LM. Temporal relationship between bone loss and increased bone turnover in ovariectomized rats [J]. Calcif Tissue Int, 1988, 43(3): 179-183.
[8] Wronski TJ, Dann LM, Scott KS, et al. Long-term effects of ovariectomy and aging on the rat skeleton [J]. Calcif Tissue Int, 1989, 45(6): 360-366.
[9] Laib A, Kumer JL, Majumdar S, et al. The temporal changes of trabecular architecture in ovariectomized rats assessed by MicroCT [J]. Osteoporos Int, 2001, 12(11): 936-941.
[10] Vignery A, Baron R. Dynamic histomorphometry of alveolar bone remodeling in the adult rat [J]. Anat Rec, 1980, 196(2): 191-200.
[11] Patullo IM, Takayama L, Patullo RF, et al. Influence of ovariectomy and masticatory hypofunction on mandibular bone remodeling [J]. Oral Dis, 2009, 15(8): 580-586.
[12] Garnero P, Sornay-Rendu E, Chapuy MC, et al. Increased bone turnover in late postmenopausal women is a major determinant of osteoporosis [J]. J Bone Miner Res, 1996, 11(3): 337-349.
[13] Kanis JA, Melton LJ 3rd, Christiansen C, et al. The diagnosis of osteoporosis [J]. J Bone Miner Res, 1994, 9(8): 1137-1141.
[14] 赵熠, 蔡育, 王贻宁. OPG/RANKL 对骨改建的调控作用 [J]. 上海口腔医学, 2010, 19(4): 443-446.
[15] Moriya Y, Ito K, Murai S. Effects of experimental osteoporosis on alveolar bone loss in rats[J]. J Oral Sci, 1998, 40(4): 171-175.
[16] Ishihara A, Sasaki T, Debari K, et al. Effects of ovariectomy on bone morphology in maxillae of mature rats [J]. J Electron Microsc (Tokyo), 1999, 48(4): 465-469.
[17] Yang J, Pham SM, Crabbe DL. Effects of oestrogen deficiency on rat mandibular and tibial microarchitecture [J]. Dentomaxillofac Radiol, 2003, 32(4): 247-251.
[18] Ejiri S, Tanaka M, Watanabe N, et al. Estrogen deficiency and its effect on the jaw bones[J]. J Bone Miner Metab, 2008, 26(5): 409-415.
[19] Tanaka M, Ejiri S, Toyooka E, et al. Effects of ovariectomy on trabecular structures of rat alveolar bone [J]. J Periodontal Res, 2002, 37(2): 161-165.
[20] Brennan O, Kuliwaba JS, Lee TC, et al. Temporal changes in bone composition, architecture, and strength following estrogen deficiency in osteoporosis[J]. Calcif Tissue Int, 2012, 91(6): 440-449.

Changes of the microarchitecture of alveolar bone due to different duration of ovariectomy: a Micro-CT study in rats

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	HUO Mei-ling, LEI Ying, XIN Xin, ZHANG Ying, WANG Rui-mei. Effect of 2 kinds of appliances on anterior tooth root and alveolar bone in patients with mild to moderate overcrowding of ClassⅠmalocclusion [J]. Shanghai Journal of Stomatology, 2024, 33(4): 407-410.
[2]	WU Tian-tian, Han Yan-yan, WANG Yi. Study of the effect of different methods on the removal of calcium hydroxide in root canal by micro-computed tomography in vivo [J]. Shanghai Journal of Stomatology, 2024, 33(3): 225-228.
[3]	LIN Hui, GAO Yun-fei, WEI Chao, WANG Meng-lei, MA Xu-hui. Effects of Sophora japonica extract on alveolar bone mass in ovariectomized osteoporosis mice [J]. Shanghai Journal of Stomatology, 2024, 33(3): 255-259.
[4]	BU Xiao-shuang, WANG Xiao, PAN Tiao-hua, ZHU Li-lei, Guo Jin-cai. Clinical effect of MB-PDT assisted initial therapy on alveolar angle absorption of lower anterior teeth [J]. Shanghai Journal of Stomatology, 2024, 33(3): 265-268.
[5]	ZHANG Min-zhen, KUANG Hui-fang, YANG Li-yan, LUO Wen. Application of small intestinal submucosa absorbable biofilm in the repair of alveolar bone defects [J]. Shanghai Journal of Stomatology, 2024, 33(3): 279-284.
[6]	CHEN Hong-li, YIN Gang, ZHANG Hai-juan. Atorvastatin promotes the healing of alveolar bone defect in rats and its effect on Wnt/β-catenin signaling pathway [J]. Shanghai Journal of Stomatology, 2024, 33(2): 130-134.
[7]	SU Juan-juan, CHEN Hong-ting, WANG Xu, JIN Qiang, WANG Lin. The relationship between the expression of PGE2 and COX-2 and the osteogenic activity and oxygen level of alveolar bone [J]. Shanghai Journal of Stomatology, 2024, 33(1): 85-89.
[8]	ZHOU Tuan-feng, YANG Xue, ZHANG Hua, ZHANG Zhi-yong, CHEN Quan. Evaluation of the 5-year clinical restoration effect of implants retained complete overdentures with Locator attachments in 48 patients [J]. Shanghai Journal of Stomatology, 2024, 33(1): 90-96.
[9]	LIU Zhen, GUO Wei-ming, LIU Lu, CHEN Rong-jing, FANG Bing. Study on promoting bone formation in osteoporotic zebrafish by lithium-doped hydroxyapatite nanowires [J]. Shanghai Journal of Stomatology, 2023, 32(5): 455-461.
[10]	YU Xiao-fei, WANG Xin-yang, LI Bin, CHEN Yao-zhong. Effects of chitosan oligosaccharide on bone metabolism and IKK/NF-κB pathway in rats with osteoporosis and periodontitis [J]. Shanghai Journal of Stomatology, 2023, 32(2): 137-142.
[11]	JU Jia, YANG Chi, WU He-ming, ZHAO Ji-hong, CHENG Li-fei, ZHANG Huan, FENG Bin, HU Kai-jin. Evaluation of the effect of cattle calcined bone grafting material to repair alveolar bone defect after tooth extraction [J]. Shanghai Journal of Stomatology, 2023, 32(2): 198-202.
[12]	LIN Xiang, FU Yu-jie, REN Gen-qiang, WEN Jia-huan, CHEN Yu-fei, ZHANG Qi. Segmentation, accuracy validation of mandibular molar, pulp cavity on cone-beam CT images by U-net neural network [J]. Shanghai Journal of Stomatology, 2022, 31(5): 454-459.
[13]	ZHOU Ming-zhi, WANG Lin, WANG Liang, WU Ke, QIN Yan-jun, ZHAO Chun-yang. Study of the mandibular changes after treatment of Class Ⅱ division 2 malocclusion in adolescents [J]. Shanghai Journal of Stomatology, 2022, 31(4): 389-394.
[14]	XIE Qin, LIN Shan, LIN Li, LI Duo. Effect of different adduction methods of maxillary incisor in adolescents on the shape of alveolar bone [J]. Shanghai Journal of Stomatology, 2022, 31(3): 290-294.
[15]	LIN Yi-jun, YAN Jie-ya, WANG Tian-ge, ZHOU Zhi-jie, LIAO Sheng-hsuan, MAO Li-xia, LIU Jia-qiang. Cone-beam CT measurement of morphological changes of the root and alveolar bone of the central incisor during orthodontic treatment with extraction [J]. Shanghai Journal of Stomatology, 2022, 31(2): 211-216.