Osteoclasts and early bone remodeling after orthodontic micro-implant placement

Abstract

Abstract: PURPOSE: To observe the incidence of osteoclasts during early bone remodeling after orthodontic micro-implant placement. METHODS：Twenty New Zealand rabbits were randomly allotted into 4 groups. One micro-implant was implanted proximal to the epiphyseal plate of the tibia. Animals were sacrificed on day 3, 7, 14 and 28 (n=5). The sequence of histological changes around the micro-implants were evaluated by hematoxylin and eosin (HE) staining. Osteoclasts were identified by TRAP staining. The differences of the number of the osteoclasts among each time point were analyzed by one way ANOVA with SPSS 19.0 software package. RESULTS: After 3 days of implantation, a large number of erythrocytes, inflammatory cells, mesenchymal cells and bone debris were seen at the implant bone interfaces. Few osteoclasts were observed. On day 7, granular woven bone was formed and some osteoclasts were found in the Howship’s lacunae. New bone formation and mineralization were apparent on day 14. Meanwhile, large amounts of osteoclasts were found in the latticed woven bone. On day 28，woven trabeculae with lamellate structures connected to lamellar bone and fewer osteoclasts were identified. Semi-quantitative analysis showed that the number of the osteoclasts was at peak on day 14. There were significant differences among each time point (P<0.01). CONCLUSIONS: Osteoclast activity is closely related to bone formation and remodeling after micro-implant insertion.

Key words: Micro-implant, Osteoclast, Bone formation, Bone remodeling

CLC Number:

R738.5

ZHANG Wei, GUO Jia-jia, ZHU Wen-qian, TANG Guo-hua. Osteoclasts and early bone remodeling after orthodontic micro-implant placement[J]. Shanghai Journal of Stomatology, 2013, 22(4): 368-373.

References

[1] Motoyoshi M, Inaba M, Ono A, et al. The effect of cortical bone thickness on the stability of orthodontic mini-implants and on the stress distribution in surrounding bone[J]. Int J Oral Maxillofac Surg, 2009, 38(1): 13-18.
[2] Wiechmann D, Meyer U, Büchter A. Success rate of mini- and micro-implants used for orthodontic anchorage: a prospective clinical study[J]. Clin Oral Implants Res,2007, 18(2): 263-267.
[3] Berglundh T, Abrahamsson I, Lang NP, et al. De novo alveolar bone formation adjacent to endosseous implants[J]. Clin Oral Implants Res, 2003, 14(3): 251-262.
[4] Wu J, Bai YX, Wang BK. Biomechanical and histomorphometric characterizations of osseointegration during mini-screw healing in rabbit tibiae[J]. Angle Orthod, 2009, 79(3): 558-563.
[5] Park IP, Kim SK, Lee SJ, et al. The relationship between initial implant stability quotient values and bone-to-implant contact ratio in the rabbit tibia[J]. J Adv Prosthodont, 2011, 3(2): 76-80.
[6] Sennerby L, Thomsen P, Ericson LE. Early tissue response to titanium implants inserted in rabbit cortical bone[J]. J Mater Sci Mater Med, 1993, 4(3): 240-250.
[7] Ek-Rylander B, Flores M, Wendel M, et al. Dephosphorylation of osteopontin and bone sialoprotein by osteoclastic tartrate-resistant acid phosphatase. Modulation of osteoclast adhesion in vitro[J]. J Biol Chem, 1994, 269(21): 14853-14856.
[8] Halleen JM, R?is?nen S, Salo JJ, et al. Intracellular fragmentation of bone resorption products by reactive oxygen species generated by osteoclastic tartrate-resistant acid phosphatase[J]. J Biol Chem, 1999, 274(33): 22907-22910.
[9] Chambers TJ. Regulation of the differentiation and function of osteoclasts[J]. J Pathol, 2000, 192(1): 4-13.
[10] Suda T, Takahashi N, Udagawa N, et al. Modulation of osteoclast differentiation and function by the new members of the tumor necrosis factor receptor and ligand families[J]. Endocr Rev, 1999, 20(3): 345-357.
[11] 柳忠豪, 周文娟, 郝鹏杰, 等. 种植体植入后牙槽骨破骨细胞的活性变化[J]. 上海口腔医学, 2012, 21(5): 511-514.
[12] 王斌, 何福明. 非负荷期种植体周围破骨细胞动态变化的组织学观察[J]. 现代实用医学, 2007, 19(8): 605-606, 620.
[13] Wu X, Deng F, Wang Z, et al. Biomechanical and histomorphometric analyses of the osseointegration of microscrews with different surgical techniques in Beagle dogs[J]. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2008, 106(5): 644-650.
[14] Deguchi T, Takano-Yamamoto T, Kanomi R, et al. The use of small titanium screws for orthodontic anchorage[J]. J Dent Res, 2003, 82(5): 377-381.
[15] Ryu J, Kim HJ, Chang EJ, et al. Sphingosine 1-phosphate as a regulator of osteoclast differentiation and osteoclast-osteoblast coupling[J]. EMBO J, 2006, 25(24): 5840-5851.
[16] Kubota K, Sakikawa C, Katsumata M, et al. Platelet-derived growth factor BB secreted from osteoclasts acts as an osteoblastogenesis inhibitory factor[J]. J Bone Miner Res, 2002, 17(2): 257-265.
[17] O’Sullivan S, Naot D, Callon K, et al. Imatinib promotes osteoblast differentiation by inhibiting PDGFR signaling and inhibits osteoclastogenesis by both direct and stromal cell-dependent mechanisms[J]. J Bone Miner Res, 2007, 22(11): 1679-1689.
[18] Kim MS, Magno CL, Day CJ, et al. Induction of chemokines and chemokine receptors CCR2b and CCR4 in authentic human osteoclasts differentiated with RANKL and osteoclast like cells differentiated by MCP-1 and RANTES[J]. J Cell Biochem, 2006, 97(3): 512-518.
[19] Pixley FJ, Stanley ER. CSF-1 regulation of the wandering macrophage: complexity in action[J]. Trends Cell Biol,2004,14(11): 628-638.
[20] Kim SH, Choi BH, Li J, et al. Peri-implant bone reactions at delayed and immediately loaded implants: an experimental study[J]. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2008, 105(2): 144-148.
[21] Zhao L, Xu Z, Yang Z, et al. Orthodontic mini-implant stability in different healing times before loading: a microscopic computerized tomographic and biomechanical analysis[J]. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2009, 108(2): 196-202.