Role of minocycline-loaded silica nanospheres in the regulation of periodontitis inflammation in rats

doi:10.19439/j.sjos.2025.03.004

Abstract

Abstract: PURPOSE: To investigate the effect of minocycline hydrochloride(MH) loaded nano-silica microspheres(MSNion) on the inflammatory regulation of periodontitis in rats. METHODS: Mesoporous silica(MSN) was prepared by classical Stöber method and MSNion was obtained by doping hydroxyapatite. MH was loaded into MSNion by magnetic stirring, and chitosan (COS), which had anti-inflammatory and antibacterial effect, was adsorbed on its surface by using charge interactions, forming MH@MSNion@COS microspheres. The microspheres were characterized by electron microscopy and X-ray diffraction. The experiments were divided into control, MH, MSNion@COS and MH@MSNion@COS groups. The cytotoxicity of each group was assessed using the CCK-8 cell assay and the optimal concentration was determined. The expression levels of inflammatory factors(TNF-α, IL-6, IL-1β, iNOS, IL-10) were determined in each group using ELISA kits. In periodontitis model, the rats were treated according to the grouping of cell experiments, periodontal probing depth (PD) and gingival index (GI) of the rats were measured at 0, 1, 2, 4 weeks. At 4 weeks of the experiment, the peripheral blood of each group of rats was collected, and the levels of inflammatory factors in serum were detected by ELISA kits. RESULTS: Nanoparticles with a particle size of about 110 nm were prepared and observed as regular spheres by electron microscopy. MH@MSNion@COS degraded into fragments with unclear structure at the 8th day. In vitro drug release assay showed a slow release of MH, and the MH release rate reached 80% at about the 15th day. In cell experiment, MH@MSNion@COS showed the best cell proliferation activity at 50 μg/mL (P<0.05), and the cell activity was higher than that of MH group and MSNion@COS group(P<0.05). There was no significant difference between MH group and MSNion@COS group. ELISA results showed that the expression of inflammatory factors in MH@MSNion@COS group was significantly lower than that in LPS group at the first day(P<0.01), and there was no significant difference between MH group and MSNion@COS group. At the 3rd day, the expression of M1 inflammatory factors in MH@MSNion@COS group was lower than that in control group, and the expression of M2 inflammatory factors was higher than that in control group(P<0.05). PD and GI of MH@MSNion@COS group were significantly decreased after administration compared with other groups(P<0.05), and the amount of inflammatory factors was lower than other groups(P<0.05). CONCLUSIONS: MH@MSNion@COS has a good inflammatory regulation effect on experimental periodontitis in vitro and in vivo.

Key words: Mesoporous silica, Nanomaterials, Periodontitis, Anti-inflammatory effect

CLC Number:

R781.3

Yang Jinxin, Ding Kexin, Sun Zhe, Cui Yawen, Liu Zongxiang. Role of minocycline-loaded silica nanospheres in the regulation of periodontitis inflammation in rats[J]. Shanghai Journal of Stomatology, 2025, 34(3): 244-250.

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