Effects of curcumin on EZH2 mRNA expression in the mandible and femur of ovariectomized osteoporosis rats JIANG Qi, ZHU Bing-yu, DEWI Chrystal, ZHOU Xiong-wen.

doi:10.19439/j.sjos.2019.03.004

Abstract

Abstract: PURPOSE: To study the effects of curcumin on EZH2 mRNA expression in the mandible and femur of ovariectomized osteoporosis rats,and to investigate its protective effect and mechanism. Methods: Thirty female 6-month old SD rats were randomly divided into sham group,OVX group and experimental group. The rats in the experimental groups were given curcumin (110 mg/kg) by intragastric administration after ovariectomy, while rats in the sham group and OVX group were given the same dosage of carboxymethylcellulose sodium solution, once a day for 12 weeks. All rats were sacrificed after the last intragastric administration. The serum samples were collected for detemination of biochemistrical parameters. Micro-CT was used for bone parameters and the morphology of the trabecular bone of the left mandibles and femurs. The expression level of EZH2mRNA in right mandible and femurs tissue was detected by reverse transcription polymerase chain reaction (RT-PCR). SPSS22.0 software package was used for statistical analysis. Results: The expression of EZH2mRNA in the OVX group was significantly higher than the sham group (P< 0.05). Compared with the OVX group,curcumin increased BMD and improved bone microstructure, decreased serum contents of alkaline phosphatase,and down-regulated the expression levels of EZH2mRNA in bone tissues of rats with osteoporosis (P< 0.05). Conclusions: Curcumin can effectively prevent the lose of bone volume of ovariectomized rats, and repaire bone microstructure. Its mechanism is related with down -regulation of EZH2mRNA.

Key words: Variectomized rats, Curcumin, Mandible, Femur, Osteoporosis, EZH2

CLC Number:

R780.2

JIANG Qi, ZHU Bing-yu, DEWI Chrystal, ZHOU Xiong-wen. Effects of curcumin on EZH2 mRNA expression in the mandible and femur of ovariectomized osteoporosis rats JIANG Qi, ZHU Bing-yu, DEWI Chrystal, ZHOU Xiong-wen.[J]. Shanghai Journal of Stomatology, 2019, 28(3): 241-245.

References

[1] Hosking SM, Dobbins AG, Pasco JA, et al.Knowledge change regarding osteoporosis prevention: translating recommended guidelines into user-friendly messages within a community forum[J]. BMC Res Notes, 2015, 8: 33-37.
[2] 陈晓, 苏佳灿. 骨质疏松研究热点:骨髓间充质干细胞分化命运[J]. 第二军医大学学报, 2017, 38(4):397-404.
[3] Kim KH, Roberts CW.Targeting EZH2 in cancer[J]. Nat Med, 2016, 22(2): 128-134.
[4] Jing H, Li L, An Y, et al.Suppression of EZH2 prevents the shift of ssteoporotic MSC fate to adipocyte and enhances bone formation during osteoporosis[J]. Mol Ther, 2016, 24(2): 217-229.
[5] Panahi Y, Ahmadi Y, Teymouri M, et al.Curcumin as a potential candidate for treating hyperlipidemia: a review of cellular and metabolic mechanisms[J]. J Cell Physiol, 2018, 233(1): 141-152.
[6] Li G, Bu J, Zhu Y, et al.Curcumin improves bone microarchitecture in glucocorticoid-induced secondary osteoporosis mice through the activation of microRNA-365 via regulating MMP-9[J]. Int J Clin Exp Pathol, 2015, 8(12): 15684-15695.
[7] Ahn J, Jeong J, Lee H, et al.Poly (lactic-co-glycolic acid) nanoparticles potentiate the protective effect of curcumin against bone loss in ovariectomized rats[J]. J Biomed Nanotechnol, 2017, 13(6): 688-698.
[8] Hussan F, Ibraheem NG, Kamarudin TA, et al.Curcumin protects against ovariectomy-induced bone changes in rat model[J]. Evid Based Complement Alternat Med, 2012, 2012: 174916.
[9] Zheng X, Zhang Y, Guo S, et al.Dynamic expression of matrix metalloproteinases 2, 9 and 13 in ovariectomy induced osteoporosis rats[J]. Exp Ther Med, 2018, 16(3): 1807-1813.
[10] Lelovas PP, Xanthos TT, Thoma SE, et al.The laboratory rat as an animal model for osteoporosis research[J]. Comp Med, 2008, 58(5): 424-430.
[11] Riva A, Franceschi F, Togni S, et al.Health benefits of curcumin and curcumin phytosome in bone density disorders[J]. Bone Marrow Res, 2017, 1(2): 1006-1009.
[12] Guo C, Li C, Yang K, et al.Increased EZH2 and decreased osteoblastogenesis during local irradiation-induced bone loss in rats[J]. Sci Rep, 2016, 6: 31318.
[13] Bhan A, Hussain I, Ansari KI, et al.Histone methyltransferase EZH2 is transcriptionally induced by estradiol as well as estrogenic endocrine disruptors bisphenol-A and diethylstilbestrol[J]. J Mol Biol, 2014, 426(20): 3426-3441.
[14] Hemming S, Cakouros D, Isenmann S, et al.EZH2, and KDM6A, act as an epigenetic switch to regulate mesenchymal stem cell lineage specification[J]. Stem Cells, 2014, 32(3): 802-815.
[15] Fang C, Yu Q, Mun SH, et al.Cutting Edge: EZH2 promotes osteoclastogenesis by epigenetic silencing of the negative regulator IRF8[J]. J Immunol, 2016, 196(11): 4452-4456.
[16] Shang W, Zhao LJ, Dong XL, et al.Curcumin inhibits osteoclastogenic potential in PBMCs from rheumatoid arthritis patients via the suppression of MAPK/RANK/c-Fos/NFATc1 signaling pathways[J]. Mol Med Rep, 2016, 14(4): 3620-3626.