聚己内酯/明胶电纺膜对MC3T3-E1成骨性能的影响

doi:10.19439/j.sjos.2025.04.001

摘要/Abstract

摘要： 目的：研究静电纺丝聚己内酯（polycaprolactone,PCL）/明胶（gelatin,GE）混纺材料作为引导骨组织再生膜对小鼠胚胎成骨细胞前体细胞MC3T3-E1成骨性能的影响。方法：利用静电纺丝技术于锡纸上制备不同比例PCL/GE纳米纤维膜,GE比例为0、30%、50%分组记为PCL、P7G3和P5G5,以纯PCL作为对照组。通过扫描电镜（SEM）、接触角测量仪、傅里叶变换红外光谱（FTIR）、X线光电子能谱（XPS）检测其形貌、成分和亲水性。采用CCK-8试剂检测MC3T3-E1在材料上的增殖情况,通过SEM、激光扫描共聚焦显微镜观察细胞黏附形态,利用碱性磷酸酶（ALP）染色、茜素红（ARS）染色观察成骨分化情况,通过qRT-PCR检测成骨相关基因表达水平。结果：SEM观察电纺膜呈纤维结构,接触角测量显示混入明胶的电纺膜亲水性提高,FTIR、XPS表征显示明胶成功加入。细胞增殖结果显示各组材料均有良好的细胞活性,P7G3组细胞增殖情况最好,差异有统计学意义。ALP、茜素红染色显示PCL/GE组对细胞成骨分化有促进作用。qRT-PCR结果显示PCL/GE材料上细胞成骨相关基因表达水平有所提升,P5G5组最佳。结论：PCL/GE电纺膜生物相容性良好,有促进细胞增殖、黏附和成骨分化的潜能,其中,P7G3组促增殖能力最强,P5G5组成骨促进作用更为显著。

关键词: 静电纺丝, 聚己内酯, 明胶, 成骨细胞

Abstract: PURPOSE: To study the effect of electrospinning polycaprolactone (PCL) /gelatin (GE) composite material as a guided bone regeneration membrane on osteogenic properties of mouse embryonic osteoblast precursor cells MC3T3-E1. METHODS: The electrospinning technology was used to fabricate different ratios of PCL/GE nanofiber membranes on tinfoil, and the GE ratios of 0%, 30% and 50% were grouped as PCL, P7G3 and P5G5, with pure PCL as the control group. The morphology, composition, and hydrophilicity were characterized using scanning electron microscopy (SEM), contact angle measurement, Fourier-transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS). CCK-8 was used to detect the proliferation of MC3T3-E1 on the material. The cell adhesion morphology was observed by SEM, confocal laser scanning microscopy (CLSM). Alkaline phosphatase (ALP) activity and alizarin red staining (ARS) were used to study osteogenic differentiation. The expression levels of osteogenic differentiation related genes were detected by quantitative real-time PCR (qRT-PCR). The data were analyzed using GraphPad Prism 8 software. RESULTS: SEM showed that the electrospun membranes exhibited a fibrous structure, contact angle measuring instrument showed increasing hydrophilicity of electrospun membranes mixed with gelatin. The successful addition of gelatin was displayed by FTIR and XPS. The results of cell proliferation indicated that all groups of materials had good cell activity, and group P7G3 had the best cell proliferation, and the difference was statistically significant. ALP, ARS showed that group PCL/GE had a promoting effect on cell osteogenic differentiation. qRT-PCR indicated that the expression level of osteogenic differentiation related genes were elevated in PCL/GE, with the best in the group of P5G5. CONCLUSIONS: The PCL/GE electrospun membranes has good biocompatibility and the potential to promote cell proliferation, adhesion, and osteogenic differentiation. Among them, the group P7G3 has the best proliferative ability, while the group P5G5 has a better effect in promoting bone formation.

Key words: Electrospinning, Polycaprolactone, Gelatin, Osteoblasts

中图分类号:

R783.1

林彦吟, 谢泽宇, 赖颖真, 吴勇敏, 高悦榕. 聚己内酯/明胶电纺膜对MC3T3-E1成骨性能的影响[J]. 上海口腔医学, 2025, 34(4): 337-345.

Lin Yanyin, Xie Zeyu, Lai Yingzhen, Wu Yongmin, Gao Yuerong. The effects of polycaprolactone / gelatin electrospun membranes on MC3T3-E1 osteogenic properties[J]. Shanghai Journal of Stomatology, 2025, 34(4): 337-345.

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