NEK2与EMT相关分子在口腔鳞状细胞癌中的表达及临床意义

doi:10.19439/j.sjos.2023.06.014

上海口腔医学 ›› 2023, Vol. 32 ›› Issue (6): 640-644.doi: 10.19439/j.sjos.2023.06.014

NEK2与EMT相关分子在口腔鳞状细胞癌中的表达及临床意义

叶婷婷, 陈蔚华, 裴婧, 贾云香, 吴海莹

南昌大学附属口腔医院病理科,江西省口腔生物医学重点实验室, 江西省口腔疾病临床医学研究中心,江西南昌 330006

收稿日期:2022-10-28 修回日期:2023-07-05 出版日期:2023-12-25 发布日期:2024-01-12
通讯作者: 陈蔚华,E-mail: 450658573@qq.com
作者简介:叶婷婷（1996-）,女,硕士,E-mail: 17663971143@163.com
基金资助:
江西省重点研发计划一般项目（S2020ZPYFB2025）

Expression and clinical significance of NEK2 and EMT related molecules in oral squamous cell carcinoma

YE Ting-ting, CHEN Wei-hua, PEI Jing, JIA Yun-xiang, WU Hai-ying

Department of Pathology, Affiliated Stomatological Hospital of Nanchang University; Key Laboratory of Oral Biomedicine, Jiangxi Province; Jiangxi Province Clinical Research Center for Oral Diseases. Nanchang 330006, Jiangxi Province, China

Received:2022-10-28 Revised:2023-07-05 Online:2023-12-25 Published:2024-01-12

摘要/Abstract

摘要： 目的: 探讨NEK2与EMT相关分子在口腔鳞状细胞癌（oral squamous cell carcinoma,OSCC）中的表达及意义。方法: 采用免疫组织化学方法检测60例原发性OSCC组织及正常口腔黏膜组织中NEK2和EMT相关分子（E-Cadherin、N-Cadherin、Vimentin）的表达水平,采用实时荧光定量PCR（qRT-PCR）检测25例OSCC组织和10例正常口腔黏膜中NEK2 mRNA的表达水平。采用SPSS 26.0软件包对数据进行统计学分析。结果: OSCC 组织中NEK2、N-Cadherin、Vimentin表达显著升高,E-Cadherin表达显著下降（P<0.05）;分化越差,NEK2表达越高,E-Cadherin表达越低（P<0.05）。结论: NEK2可作为初步判断OSCC预后的标志物。NEK2的表达上调,EMT相关分子表达水平的变化导致细胞间黏附力下降,侵袭性增强,促进肿瘤的发生、发展。

关键词: 口腔鳞状细胞癌, NEK2, EMT, 预后

Abstract: PURPOSE: To investigate the expression and significance of NEK2 and EMT-related molecules in oral squamous cell carcinoma (OSCC). METHODS: The expression levels of NEK2 and EMT-related molecules (E-Cadherin, N-Cadherin, Vimentin) in 60 cases of primary OSCC tissues and normal oral mucosa tissues were detected by immunohistochemistry(IHC). NEK2 mRNA expression in 25 OSCC tissues and 10 normal oral mucosa tissues was detected by qRT-PCR. Statistical analysis was performed using SPSS 26.0 software package. RESULTS: The expression of NEK2, N-Cadherin, and Vimentin increased in OSCC tissues, while the expression of E-Cadherin decreased(P<0.05). The worse the differentiation, the higher the expression of NEK2 and the lower the expression of E-Cadherin(P<0.05). CONCLUSIONS: NEK2 can be used as a prognostic marker for OSCC. The up-regulation of NEK2 and the changes in the expression levels of EMT related molecules can promote the occurrence and development of OSCC.

Key words: Oral squamous cell carcinoma, NEK2, EMT, Prognosis

中图分类号:

R739.8

叶婷婷, 陈蔚华, 裴婧, 贾云香, 吴海莹. NEK2与EMT相关分子在口腔鳞状细胞癌中的表达及临床意义[J]. 上海口腔医学, 2023, 32(6): 640-644.

YE Ting-ting, CHEN Wei-hua, PEI Jing, JIA Yun-xiang, WU Hai-ying. Expression and clinical significance of NEK2 and EMT related molecules in oral squamous cell carcinoma[J]. Shanghai Journal of Stomatology, 2023, 32(6): 640-644.

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NEK2与EMT相关分子在口腔鳞状细胞癌中的表达及临床意义

Expression and clinical significance of NEK2 and EMT related molecules in oral squamous cell carcinoma

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