BRAF V600E在成釉细胞瘤、成釉细胞癌和囊肿中的表达

doi:10.19439/j.sjos.2023.06.012

上海口腔医学 ›› 2023, Vol. 32 ›› Issue (6): 630-634.doi: 10.19439/j.sjos.2023.06.012

BRAF V600E在成釉细胞瘤、成釉细胞癌和囊肿中的表达

裴婧¹, 张林², 贾云香¹, 陈蔚华¹

1.南昌大学附属口腔医院病理科,2.修复科,江西省口腔生物医学重点实验室, 江西省口腔疾病临床医学研究中心,江西南昌 330006

收稿日期:2022-11-08 修回日期:2023-01-15 出版日期:2023-12-25 发布日期:2024-01-12
通讯作者: 陈蔚华,E-mail: 450658573@qq.com
作者简介:裴婧(1984-),女,硕士,副主任医师,E-mail: peijingzi@163.com
基金资助:
江西省卫生健康委员会科技计划（20204404）

BRAF V600E expression in ameloblastomas, ameloblastic carcinomas and cysts

PEI Jing¹, ZHANG Lin², JIA Yun-xiang¹, CHEN Wei-hua¹

1. Department of Pathology, 2. Department of Prosthodontics, Affiliated Stomatological Hospital of Nanchang University; Key Laboratory of Oral Biomedicine, Jiangxi Province; Jiangxi Province Clinical Research Center for Oral Diseases. Nanchang 330006, Jiangxi Province, China

Received:2022-11-08 Revised:2023-01-15 Online:2023-12-25 Published:2024-01-12

摘要/Abstract

摘要： 目的: 探讨BRAF V600E表达是否与成釉细胞瘤复发、恶变有关。方法: 运用免疫组织化学和实时荧光定量PCR（qPCR）检测成釉细胞瘤、成釉细胞癌、囊肿中BRAF V600E的蛋白和基因表达水平。采用SPSS 26.0软件包对数据进行统计学分析。结果: BRAF V600E在原发性成釉细胞瘤和复发性成釉细胞瘤中的表达无显著差异,在囊肿和成釉细胞癌中的表达显著减少。免疫组织化学和RT-qPCR检测的阳性率一致。结论: 成釉细胞瘤中BRAF V600E的蛋白表达与年龄、性别、部位、大小、组织学类型以及是否复发无关,BRAF V600E的表达对鉴别成釉细胞瘤、囊肿、成釉细胞癌有一定指导意义。使用免疫组织化学方法或许能初步检测成釉细胞瘤中是否存在BRAF突变。

关键词: 成釉细胞瘤, 成釉细胞癌, BRAF V600E, 复发

Abstract: PURPOSE: To investigate protein and genetic status of BRAF V600E in ameloblastomas, ameloblastic carcinomas and cysts, and to explore if recurrence and malignant transformation of ameloblastomas depends on BRAF status. METHODS: Twenty cysts, 25 primary ameloblastomas, 25 recurrent ameloblastomas and 8 ameloblastic carcinomas were analysed by immunohistochemistry, and 2 cysts, 5 primary ameloblatomas, 5 recurrent ameloblastomas, 3 atypical ameloblastomas and 6 ameloblastic carcinomas were analysed by quantitative real-time PCR(qPCR). SPSS 26.0 software package was used for data analysis. RESULTS: There was no correlation between BRAF V600E expression and recurrence. Cysts and carcinomas had lower frequencies of BRAF V600E mutations than ameloblastomas. There was a concordance between positive staining of BRAF V600E protein and BRAF V600E mutation. CONCLUSIONS: BRAF V600E mutation may be useful in the differential diagnosis of ameloblastomas with cysts and ameloblastic carcinomas. Immunohistochemistry may be a beneficial method to screen for BRAF V600E mutation in ameloblastoma.

Key words: Ameloblastoma, Ameloblastic carcinoma, BRAF V600E, Recurrence

中图分类号:

R739.8

裴婧, 张林, 贾云香, 陈蔚华. BRAF V600E在成釉细胞瘤、成釉细胞癌和囊肿中的表达[J]. 上海口腔医学, 2023, 32(6): 630-634.

PEI Jing, ZHANG Lin, JIA Yun-xiang, CHEN Wei-hua. BRAF V600E expression in ameloblastomas, ameloblastic carcinomas and cysts[J]. Shanghai Journal of Stomatology, 2023, 32(6): 630-634.

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BRAF V600E在成釉细胞瘤、成釉细胞癌和囊肿中的表达

BRAF V600E expression in ameloblastomas, ameloblastic carcinomas and cysts

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