iRoot FM对牙髓卟啉单胞菌抗菌效果评价

doi:10.19439/j.sjos.2017.05.005

上海口腔医学 ›› 2017, Vol. 26 ›› Issue (5): 488-491.doi: 10.19439/j.sjos.2017.05.005

iRoot FM对牙髓卟啉单胞菌抗菌效果评价

毕静¹, 刘尧^{1, 2}, 陈旭^{1, 2}

1.中国医科大学口腔医学院儿童口腔科,辽宁沈阳 110002；
2.辽宁省口腔疾病重点实验室,辽宁沈阳 110002

收稿日期:2017-03-13 修回日期:2017-05-03 出版日期:2017-10-25 发布日期:2017-11-23
通讯作者: 陈旭,E-mail: chenxu_cmu@sina.com
作者简介:毕静（1991-）,女,在读硕士研究生,E-mail: bijing1124@126.com
基金资助:
辽宁省高等学校基本科研项目（LQNK201723）; 沈阳市科学技术计划项目应用基础研究专项（F16-205-1-25）

Antimicrobial effect of a new bio-ceramic material iRoot FM on Porphyromonas endodontalis

BI Jing¹, LIU Yao^{1, 2}, CHEN Xu^{1, 2}

1.Department of Pediatric Dentistry, School of Stomatology, China Medical University. Shenyang 110002;
2.Liaoning Provincial Key Laboratory of Oral Disease. Shenyang 110002, Liaoning Province, China

Received:2017-03-13 Revised:2017-05-03 Online:2017-10-25 Published:2017-11-23

摘要/Abstract

摘要： 目的比较iRoot FM与传统根管消毒材料氢氧化钙[Ca(OH)₂]及三联抗生素糊剂（triple antibiotic paste, TAP）对牙髓卟啉单胞菌（Porphyromonas endodontalis, P. endodontalis）的抗菌效果,评价iRoot FM的抗菌性能,为临床上根管消毒材料的选择提供参考。方法采用国际标准菌株P. endodontalis ATCC35406进行琼脂平板扩散实验。实验组为iRoot FM组、Ca(OH)₂组及TAP组,以蒸馏水作为空白对照组。将冻干的P. endodontalis复苏,増菌,接种于培养皿中,干燥后打孔,将材料分别加入小孔中,37℃厌氧箱培养72 h,测定抑菌环直径（mm）。实验重复6次（n=6）,采用SPSS17.0软件包对数据进行统计学分析。结果iRoot FM、Ca(OH)₂及TAP 3组抑菌环直径分别为（20.74±4.35）mm、（24.89±3.84）mm和（34.51±1.20）mm。其中,iRoot FM组与Ca(OH)₂组相比,无显著差异（P>0.05）;而iRoot FM组、Ca(OH)₂组与TAP组相比,差异具有显著性（P<0.05）。对照组无抑菌环出现。结论新型生物陶瓷材料iRoot FM在体外实验中对牙髓卟啉单胞菌具有较强的抗菌作用,提示其在根管消毒中具有良好的应用前景。

关键词: 根管消毒材料, iRoot FM, 牙髓卟啉单胞菌, 抗菌性能

Abstract: PURPOSE: To compare the antimicrobial effect of a new bio-ceramic material iRoot FM with traditional intracanal medicaments including calcium hydroxide and triple antibiotic paste (TAP), on Porphyromonas endodontalis (P. endodontalis), and to evaluate the antimicrobial activity of iRoot FM, providing reference for clinical use of intracanal medicaments. METHODS: P. endodontalis ATCC 35406 were used in this study. The study was divided into 3 experimental groups including iRoot FM, and TAP group. Sterile water was used as blank control. Frozen P. endodontalis were seeded and grown overnight in the liquid medium, then P. endodontalis were seeded on BHI-blood agar plates. After the plates were dried, the materials were filled on the plates which were made by a punching machine. Zones of inhibition (mm) were measured after 72 h of anaerobic incubation at 37℃. The experimental data were analyzed statistically using SPSS 17.0 software package. RESULTS: The zones of inhibition in the 3 experimental groups: iRoot FM, and TAP group were (20.74±4.35)mm, (24.89±3.84)mm and (34.51±1.20)mm, respectively. The zones of inhibition of the iRoot FM group and group were significantly smaller compared with the TAP group (P<0.05), while there was no significant difference between the iRoot FM group and Ca(OH)₂ groups (P>0.05). There were no zone of inhibition in the control group. CONCLUSIONS: As a new bio-ceramic material, iRoot FM shows a good antimicrobial activity against P. endodontalis and may be a promising intracanal material.

Key words: Intracanal medicaments, iRoot FM, Porphyromonas endodontalis, Antimicrobial activity

中图分类号:

R783.1

毕静, 刘尧, 陈旭. iRoot FM对牙髓卟啉单胞菌抗菌效果评价[J]. 上海口腔医学, 2017, 26(5): 488-491.

BI Jing, LIU Yao, CHEN Xu. Antimicrobial effect of a new bio-ceramic material iRoot FM on Porphyromonas endodontalis[J]. Shanghai Journal of Stomatology, 2017, 26(5): 488-491.

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iRoot FM对牙髓卟啉单胞菌抗菌效果评价

Antimicrobial effect of a new bio-ceramic material iRoot FM on Porphyromonas endodontalis

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