白藜芦醇对高糖环境下牙龈上皮细胞TLR4表达及炎症因子分泌的影响

doi:10.19439/j.sjos.2017.01.007

上海口腔医学 ›› 2017, Vol. 26 ›› Issue (1): 32-36.doi: 10.19439/j.sjos.2017.01.007

白藜芦醇对高糖环境下牙龈上皮细胞TLR4表达及炎症因子分泌的影响

吕佳姝¹, 蒋雪薇¹, 章燕², 甄蕾¹

1.上海市口腔病防治院牙周病科,上海 200001;
2.同济大学附属口腔医院转化医学实验室,上海 200072

收稿日期:2016-06-27 修回日期:2016-09-22 出版日期:2017-02-25 发布日期:2017-03-20
通讯作者: 甄蕾,E-mail:zhenleilei123@126.com
作者简介:吕佳姝（1981-）,女,硕士,主治医师,E-mail:jiashu@126.com
基金资助:
上海市自然科学基金（13ZR1436300）; 上海市卫生和计划生育委员会面上项目（201640103）

Effect of resveratrol on expression of TLR4 and inflammatory factors in gingival epithelial cells under high glucose environment

LV Jia-shu¹, JIANG Xue-wei¹, ZHANG Yan², ZHEN Lei¹

1.Department of Periodontology, Shanghai Stomatological Disease Center. Shanghai 200001;
2.Department of Translational Medicine Laboratory, Shanghai Tongji Hospital, Tongji University School of Medicine. Shanghai 200072, China

Received:2016-06-27 Revised:2016-09-22 Online:2017-02-25 Published:2017-03-20

摘要/Abstract

摘要： 目的研究白藜芦醇对高糖环境下牙龈上皮细胞TLR4表达及炎症因子分泌的影响,探讨白藜芦醇对伴糖尿病牙周炎患者的治疗作用及相关分子机制。方法体外培养牙龈上皮细胞,按照作用方式不同分为正常对照组、高糖组和高糖+白藜芦醇组。荧光定量PCR检测TLR4表达情况;取第3代牙龈上皮细胞,高糖环境下采用或不采用白藜芦醇处理24 h,随后用100 ng/mL LPS处理2 h,ELISA检测IL-1β、IL-6、IL-8 和 TNF-α分泌;Western 印迹法检测TLR4信号通路下游分子NF-κB p65、 p38MAPK 和 STAT3磷酸化。采用SPSS 17.0软件包对数据进行统计学分析。结果白藜芦醇可以逆转高糖环境下牙龈上皮细胞TLR4水平的升高,同时抑制高糖环境下LPS诱导的IL-1β、 IL-6、IL-8 和 TNF-α表达。Western 印迹结果显示,白藜芦醇也可抑制TLR4信号通路下游分子NF-κB p65、 p38MAPK 和 STAT3磷酸化。结论白藜芦醇通过负向调控TLR4信号通路减轻高糖环境下牙龈上皮细胞炎症因子的分泌。

关键词: 白藜芦醇, 糖尿病, 牙周炎, 牙龈上皮细胞, 炎性细胞因子, TLR4

Abstract: PURPOSE: Through a study of the molecular mechanism of the effect of resveratrol(RSV) on expression of TLR4 and inflammatory factors in gingival epithelial cells under high glucose environment, the therapeutic effect and molecular mechanism of resveratrol on periodontitis in patients with diabetes mellitus was investigated. METHODS: Gingival epithelial cells were cultured in vitro; according to the way of action, the cultured cells were divided into control group, high glucose group(HG) and HG+RSV group. The mRNA expression of TLR4 was detected by PCR; The third generation of gingival epithelial cells were pre-treated with or without RSV for 24 h under high glucose conditions, and subsequently treated with LPS at 100 ng/mL for 2 h. ELISA was used to detect the secretion of IL-1 beta, IL-6, IL-8 and TNF- alpha; the activation of TLR4 downstream signaling molecules NF-κB p65, p38 MAPK, and STAT3 was determined by Western blot. SPSS17.0 software package was used for statistical analysis. RESULTS: RSV could reverse the increase of TLR4 level in gingival epithelial cells in high glucose medium.LPS markedly increased the expression and secretion of IL-1β, IL-6, IL-8, and TNF-α in GECs cultured in high glucose medium, which was partly blocked in the presence of RSV. Furthermore, Western blot results showed that RSV significantly suppressed the phosphorylation of TLR4 downstream factors NF-κB p65, p38MAPK, and STAT3. CONCLUSIONS: RSV reduces inflammatory cytokine secretion in gingival epithelial cells, through negative regulation of TLR4 signaling pathway.

Key words: Resveratrol, Diabetes, Periodontitis, Gingival epithelial cell, Inflammatory factor, TLR4

中图分类号:

R781.4

吕佳姝, 蒋雪薇, 章燕, 甄蕾. 白藜芦醇对高糖环境下牙龈上皮细胞TLR4表达及炎症因子分泌的影响[J]. 上海口腔医学, 2017, 26(1): 32-36.

LV Jia-shu, JIANG Xue-wei, ZHANG Yan, ZHEN Lei. Effect of resveratrol on expression of TLR4 and inflammatory factors in gingival epithelial cells under high glucose environment[J]. Shanghai Journal of Stomatology, 2017, 26(1): 32-36.

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白藜芦醇对高糖环境下牙龈上皮细胞TLR4表达及炎症因子分泌的影响

Effect of resveratrol on expression of TLR4 and inflammatory factors in gingival epithelial cells under high glucose environment

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