MMPs抑制剂对湿性黏结微渗漏的影响

上海口腔医学 ›› 2016, Vol. 25 ›› Issue (5): 548-552.

MMPs抑制剂对湿性黏结微渗漏的影响

赵聪, 迟爽, 李彤, 李贺, 李天博, 张志民

吉林大学口腔医学院,吉林长春 130021

收稿日期:2015-11-13 修回日期:2016-01-14 出版日期:2016-10-25 发布日期:2016-11-10
通讯作者: 张志民,E-mail：zhangzm1964@sina.com
作者简介:赵聪（1993-）,女,学士,E-mail：10124566@qq.com

Effects of MMPs inhibitors on microleakage with wet bonding technique

ZHAO Cong, CHI Shuang, LI Tong, LI He, LI Tian-bo, ZHANG Zhi-min

School of Stomatology, Jilin University. Changchun 130021, Jilin Province, China

Received:2015-11-13 Revised:2016-01-14 Online:2016-10-25 Published:2016-11-10

摘要/Abstract

摘要： 目的研究牙本质湿性黏结时,基质金属蛋白酶(matrix metalloproteinases,MMPs)抑制剂对黏结界面微渗漏的影响。方法收集人离体前磨牙27颗,平均分成3组。将牙体颈部统一制备V类窝洞,酸蚀后分别用蒸馏水、2%氯己定(chlorhexidine,CHX)和2%米诺环素(monocycline,MI)在窝洞内均匀涂抹60 s,然后黏结、树脂填充、固化。用10%次氯酸钠(sodium hypochlorite,NaClO)溶液老化1 h,再涂布2层指甲油,50wt%氨化硝酸银(ammoniated silver nitrate,ASN)和显影液处理各组牙。最后将牙切成1~2 mm的牙片,应用体式显微镜和扫描电镜观察充填树脂与牙本质之间银颗粒渗入情况,采用SPSS 16.0 软件包对各组微渗漏程度进行Wilcoxon秩和检验。结果各组充填树脂周边均有不同程度的微渗漏情况。经Wilcoxon秩和检验,CHX组与对照组、MI组与对照组的差异有显著性;CHX组与MI组相比,差异无显著性。结论牙体湿性黏结时,MMPs抑制剂能减轻黏结界面微渗漏的程度。

关键词: 牙本质黏结, 基质金属蛋白酶抑制剂, 氯己定, 米诺环素, 微渗漏

Abstract: PURPOSE: To observe the effects of matrix metalloproteinasesMMPs inhibitors on microleakage with wet bonding technique. METHODS: Twenty-seven premolars were randomly assigned to 3 groups. Class Ⅴ cavities were prepared in the neck of the teeth and treated with distilled water, 2% chlorhexidine CHX or 2% monocycline MI for 60 seconds. Resin was used to restore the prepared cavities following application of luting agent. Samples in each group were soaked for 1 h with 10% NaClO and then 2 layers of nail polish were applied, followed by treatment with 50wt% ammoniated silver nitrate ASN, and developing solution in turn. Each tooth was sectioned longitudinally to 1~2 mm slices and examined with stereo microscope and scanning electron microscope SEM. The data were analyzed with SPSS 16.0 software package for Wilcoxon rank sum test. RESULTS: There was varied microleakage at the margin. The difference was significant between group CHX and control group, between group MI and control group; However, there was no significant difference between group CHX and group MI. CONCLUSIONS: MMPs inhibitor can reduce microleakage with wet bonding technique.

Key words: Dentin bonding, Matrix metalloproteinases inhibitor, Chlorhexidine, Monocycline, Microleakage

中图分类号:

R783.1

赵聪, 迟爽, 李彤, 李贺, 李天博, 张志民. MMPs抑制剂对湿性黏结微渗漏的影响[J]. 上海口腔医学, 2016, 25(5): 548-552.

ZHAO Cong, CHI Shuang, LI Tong, LI He, LI Tian-bo, ZHANG Zhi-min. Effects of MMPs inhibitors on microleakage with wet bonding technique[J]. Shanghai Journal of Stomatology, 2016, 25(5): 548-552.

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