OPG/RANK/RANKL在牙周炎联合血管钙化大鼠牙髓组织中的表达及意义

上海口腔医学 ›› 2016, Vol. 25 ›› Issue (4): 391-395.

OPG/RANK/RANKL在牙周炎联合血管钙化大鼠牙髓组织中的表达及意义

潘克清¹, 张朋梅^{1, 2}, 邓婧¹, 娄秀秀¹, 孟芸³, 刘桂荣⁴

1.青岛大学附属医院口腔科,山东省口腔临床医学重点实验室,山东青岛 266003; 2.青岛慧康医院口腔科,山东青岛 266000;.天津市东丽区中医医院口腔科,天津 300300; 4.青岛市市立医院口腔科,山东青岛 266011

收稿日期:2015-09-16 出版日期:2016-08-25 发布日期:2016-09-06
通讯作者: 潘克清,E-mail:pankeqing77@sina.com
作者简介:潘克清(1977-),女,博士研究生,医师
基金资助:
国家自然科学基金（81100755）; 山东省口腔生物医学重点实验室开放课题（SDKQ201401）

Expression of OPG/RANK/RANKL in the rat dental pulp tissue of periodontitis combined with vascular calcification and its clinical significance

PAN Ke-qing¹, ZHANG Peng-mei^{1, 2}, DENG Jing¹, LOU Xiu-xiu¹, MENG Yun³, LIU Gui-rong⁴

1.Department of Stomatology, Affiliated Hospital of Qingdao University;Key Laboratory of Clinical Stomatology. Qingdao 266003, Shandong Province;
2.Department of Stomatology, Qingdao Huikang Hospital.Qingdao 266000, Shandong Province;;
3.Department of Stomatology,Chinese Medical Hospital of Dongli. Tianjin 300300;;
4.Department of Stomatology,Qingdao Municipal Hospital.Qingdao 266011, Shandong Province,China

Received:2015-09-16 Online:2016-08-25 Published:2016-09-06

摘要/Abstract

摘要： 目的研究OPG/RANK/RANKL在牙周炎联合血管钙化大鼠牙髓组织中的表达及其可能的作用。方法取36只SPF级雄性Wistar大鼠,随机分为4组,正常对照组（C组）、牙周炎组(CP组)、血管钙化组（VDN组）和牙周炎血管钙化联合组（CP+VDN组）,并按照相应的方法建立动物模型。模型建立成功后,取含上颌第二磨牙的上颌骨, H-E染色观察牙髓组织的变化;免疫组织化学Envision法检测牙髓组织中OPG和RANKL蛋白质的表达及OPG/RANKL比值的变化。采用SPSS19.0软件包对数据进行统计学分析。结果牙髓组织H-E染色观察显示,CP组、VDN组和CP+VDN组牙髓组织不同程度破坏,牙髓内可见中性粒细胞浸润,牙髓血管充血,成牙本质细胞空泡性变,牙髓坏死。其中,CP+VDN组最为显著,其次为CP组、VDN组。免疫组织化学染色结果显示,C组大鼠牙髓组织OPG强阳性表达,CP组、VDN组和CP+VDN组的平均吸光度值显著低于正常对照组（P<0.05),其中以CP+VDN组OPG阳性表达率最低;CP组、VDN组和CP+VDN组大鼠牙髓组织RANKL强阳性表达,各实验组的平均吸光度均显著高于C组（P<0.05),其中CP+VDN组RANKL阳性表达率最高;OPG/RANKL值C组最高,CP+VDN组最低,差异显著（P<0.05)。结论牙周炎和血管钙化均对牙髓组织产生一定损伤,牙周炎合并血管钙化可加重这一损伤;OPG/RANKL/RANK可能在牙周炎和血管钙化对牙髓组织的影响中发挥重要作用。

关键词: 牙周炎, 血管钙化, 牙髓组织, OPG/RANK/RANKL

Abstract: PURPOSE: To study the expression and possible role of OPG/RANK/RANKLin the rat dental pulp of periodontitis combined with vascular calcification. METHODS: Thirty-six male Wister rats were randomly divided into 4 groups: control group(group C), periodontitis group(group CP), vascular calcification group(group VDN) and compound group(group CP+VDN). Each group underwent corresponding management to establish animal model. When the model was successful, the maxillae including molars were sectioned, pulp tissue was examined by H-E staining; Immunohistochemical staining method was used to evaluate the expression and ratio of OPG and RANKL in pulp tissues. Statistical analysis was carried out using SPSS 19.0 software package. RESULTS: The pulp tissue of group CP, VDN, CP+VDN showed varied degrees of damage, neutrophil infiltration, pulp vascular congestion, odontoblasts vacuolar changes, pulp necrosis by H-E staining, and the changes in CP+VDN group was the most significant, followed by CP group, VDN group. Immunohistochemistry showed OPG in pulp tissues in group CP, VDN, CP+VDN were significantly lower than that in normal group (P<0.05), and the expression in group CP+VDN was the least;Expression of RANKL in pulp tissues in group CP, VDN, CP+VDN were significantly higher than that in normal group(P<0.05),and the expression in group CP+VDN was the highest. The ratio of OPG/RANKL in normal group was the highest, and the ratio in CP+VDN group was the lowest. CONCLUSIONS: Periodontitis and vascular calcification can damage the pulp tissue, periodontitis compound with vascular calcification may aggravate the injury; OPG/RANKL/RANK system may play an important role in pulp tissue injury.

Key words: Periodontitis, Vascular calcification, Pulp tissue, OPG/RANK/RANKL

中图分类号:

R781.4

潘克清, 张朋梅, 邓婧, 娄秀秀, 孟芸, 刘桂荣. OPG/RANK/RANKL在牙周炎联合血管钙化大鼠牙髓组织中的表达及意义[J]. 上海口腔医学, 2016, 25(4): 391-395.

PAN Ke-qing, ZHANG Peng-mei, DENG Jing, LOU Xiu-xiu, MENG Yun, LIU Gui-rong. Expression of OPG/RANK/RANKL in the rat dental pulp tissue of periodontitis combined with vascular calcification and its clinical significance[J]. Shanghai Journal of Stomatology, 2016, 25(4): 391-395.

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