种植体型功能矫治器矫治过程中Beagle犬咬肌肌纤维类型的变化

上海口腔医学 ›› 2016, Vol. 25 ›› Issue (1): 22-26.

种植体型功能矫治器矫治过程中Beagle犬咬肌肌纤维类型的变化

孙旭容¹，林新平²，操亚波¹

1.杭州口腔医院正畸中心，浙江杭州 310006；
2.浙江中医药大学，浙江杭州 310016

收稿日期:2015-03-13 修回日期:2015-05-24 出版日期:2016-02-25 发布日期:2016-03-09
通讯作者: 林新平， E-mail：xplinzju@163.com E-mail:sxr816@163.com
作者简介:孙旭容（1979-），女，硕士，主治医师， Tel：0571-28281656
基金资助:
温州市国际合作科技计划项目(B00260027)

Fibre-type differences in masseter muscle of Beagle dogs with implanted functional appliance therapy

SUN Xu-rong¹, LIN Xin-ping², CAO Ya-bo¹.

1.Department of Orthodontics，Hangzhou Stomatology Hospital. Hangzhou 310006;
2.Zhejiang Chinese Medical University. Hangzhou 310016,Zhejiang Province,China

Received:2015-03-13 Revised:2015-05-24 Online:2016-02-25 Published:2016-03-09

摘要/Abstract

摘要： 目的： 观察种植体型功能矫治器引导Beagle犬下颌骨前移治疗过程中咬肌MyHC-I表达量的变化，探讨种植体型功能矫治器矫治过程中咀嚼肌的适应性改建，为矫形治疗提供理论依据。方法： 8只7个月龄雄性Beagle犬分别在安装矫治器治疗后4、8、12周及拆除矫治器后4周（即16周）处死，取咬肌浅层中部组织做切片，用SABC法检测 MyHC-I在Beagle犬咬肌中的表达，采用SPSS11.5软件包对数据进行统计学分析。结果： MyHC-I在对照组咬肌中的阳性表达率为7.61%，在4周实验组咬肌中的阳性表达率为20.43%﹙P<0.01﹚，8周时达到最大，而后逐渐减少。拆除矫治器后，咬肌中MyHC-I的表达逐渐减少，直至与对照组水平接近。结论： 种植体型功能矫治器前移下颌过程中咬肌MyHC-I的表达出现明显变化，种植体型功能矫治器能明显促进咀嚼肌的适应性改建。

关键词: 种植体, 功能矫治器, 咬肌

Abstract: PURPOSE: To observe the changes of MyHC-I expression in masseter muscle of Beagle dogs with implanted functional appliance therapy，and to investigate the adaptive remodeling of masticatory muscle. METHODS： Eight, seven-month-old, male Beagle dogs were randomly assigned to experimental group and control group and were killed at 4,8,12 weeks during treatment and 4 weeks after the appliance removed(16 weeks). Serial cross-sections of superficial masseter muscle were cut and stained with MyHC-I antibodies.SPSS11.5 software package was used for statistical analysis. RESULTS: The positive expression rate of MyHC-I was 7.61% in the control group, and 20.43% in the experimental groups at 4 weeks﹙P<0.01﹚. The amount of type I fibres reached to peak at 8 weeks. With increase of treatment time, the amount of type I fibres gradually reduced. At 16 weeks, the amount of type I fibres was similar to the control groups. CONCLUSIONS: The expression of MyHC-I is significantly changed in masseter muscles of Beagle dogs with implanted functional appliance therapy. Implanted functional appliance forward mandibular displacement therapy can obviously promote the adaptive remodeling of masticatory muscle.

Key words: Implant, Functional appliance, Masseter muscle

中图分类号:

R873.5

孙旭容，林新平，操亚波. 种植体型功能矫治器矫治过程中Beagle犬咬肌肌纤维类型的变化[J]. 上海口腔医学, 2016, 25(1): 22-26.

SUN Xu-rong, LIN Xin-ping.. Fibre-type differences in masseter muscle of Beagle dogs with implanted functional appliance therapy[J]. Shanghai Journal of Stomatology, 2016, 25(1): 22-26.

参考文献

[1] 林新平, 林久祥, 王剑锋, 等. 种植体型功能矫治器对颅面部生长作用的三维结构分析 [J]. 上海口腔医学, 2008, 17(1): 71-76.
[2] Sano R, Tanaka E, Korfage JA, et a1. Heterogeneity of fiber characteristics in the rat masseter and digastric muscles[J]. J Anat, 2007, 21(1): 464-470.
[3] Acevedo LM, Rivero JL. New insights into skeletal muscle fibre types in the dog with particular focus towards hybrid myosin phenotypes [J]. Cell Tissue Res, 2006, 323(2): 283-303.
[4] 李伯江, 李平华, 吴望军, 等. 骨骼肌肌纤维形成机制的研究进展 [J].中国农业科学, 2014, 47(6): 1200-1207.
[5] Ohmae M, Saito S, Morohashi T, et al. A clinical and histological evaluation of titanium mini-implants as anchors for orthodontic intrusion in the beagle dog [J]. Am J Orthod Dentofacial Orthop, 2001, 119(5): 489-497.
[6] Saito S, Sugimoto N, Morohashi T, et al. Endosseous titanium implants as anchors for mesiodistal tooth movement in the beagle dog [J]. Am J Orthod Dentofacial Orthop, 2000, 118(6): 601-607.
[7] 刘静,张跃蓉,张永,等.人咬肌和颞肌的肌构筑及生物学特性 [J]. 实用口腔医学杂志, 2002, 18(4): 294-297.
[8] Pae EK. Response of masticatory muscles to passive stretch stimulus-from perspectives of functional appliances [J]. Korean J Orthod, 2012, 42(2): 64-72.
[9] Gedrange T, Luck O, Hesske G, et al. Differential expression of myosin heavy-chain mRNA in muscles of mastication during functional advancement of the mandible in pigs[J]. Arch Oral Biol, 2001, 46(3): 215-220.
[10] Korfage JA, Koolstra JH, Langenbach GE, et al. Fiber-type composition of the human jaw muscles--(part 1) origin and functional significance of fiber-type diversity[J]. J Dent Res, 2005, 84(9): 774-783.
[11] Grünheid T, Langenbach GE, Korfage JA, et al. The adaptive response of jaw muscles to varying functional demands[J]. Eur J Orthod, 2009, 31(6): 596-612.
[12] Kawni N, Tanaka E, Langenbach GE, et at. Daily jaw muscle activity in freely moving rats measured with radio-telemetry[J]. Eur J Oral SCi, 2007, 115(1): 15-20.
[13] Gelhaye M, Martrette JM, Legrand-Frossi C, et al. Myosin heavy chain expression and muscle adaptation to chronic oral breathing in rat [J]. Respir Physiol Neurobiol, 2006, 154(3): 443-452.
[14] Sciote JJ, Horton MJ, Rowlerson AM, et al. Human masseter muscle fiber type properties, skeletal malocclusions, and muscle growth factor expression[J].J Oral Maxillofac Surg, 2012, 70(2): 440-448.
[15] Gedrange T, Walter B, Tetzlaff I, et al. Regional alterations in fiber type distribution, capillary density, and blood flow after lower jaw sagittal advancement in pig masticatory muscles[J]. J Dent Res, 2003, 82(7): 570-574.