雌激素降低对大鼠髁突软骨ERα、ERβ、OPG和RANKL表达的影响

上海口腔医学 ›› 2015, Vol. 24 ›› Issue (4): 437-441.

雌激素降低对大鼠髁突软骨ERα、ERβ、OPG和RANKL表达的影响

李振兴, 陈传俊

安徽医科大学第三附属医院口腔颌面外科,安徽合肥 230061

收稿日期:2014-09-28 出版日期:2015-08-20 发布日期:2015-09-10
通讯作者: 陈传俊,Tel：0551-62183622, E-mail：ccj6318@sina.com E-mail:ccj6318@sina.com
作者简介:李振兴 (1990-),男,在读硕士研究生,E-mail：824682775@qq.com
基金资助:
合肥市科技计划资助项目 [合科 (2013) 183号-37]

The effect of lowered serum levels of estrogen on the expression of ERα, ERβ, OPG and RANKL in rat mandibular condylar cartilage

LI Zhen-xing, CHEN Chuan-jun

Department of Oral and Maxillofacial Surgery, the 3rd Affiliated Hospital of Anhui Medical University. Hefei 230061, Anhui Province, China

Received:2014-09-28 Online:2015-08-20 Published:2015-09-10
Supported by:
Supported by Science and Technology Plan Project of Hefei City [(2013)183-37]

摘要/Abstract

摘要： 目的观察血清雌激素降低后大鼠髁突软骨ERα、ERβ、OPG和RANKL的表达情况,探讨髁突特发性吸收可能的发生机制。方法30只雌性SD大鼠随机分为对照组、避孕药组和雷公藤多苷片组。实验组通过灌胃避孕药（去氧孕烯炔雌醇片）和雷公藤多苷片2种方法建立大鼠血清雌激素降低模型。在实验前和实验第12周,分别检测血清雌二醇浓度,实验结束后,应用免疫组织化学法检测髁突软骨ERα、ERβ、OPG和RANKL的表达。采用SPSS16.0软件包对数据进行统计学分析。结果避孕药组和雷公藤多苷片组血清雌激素水平均明显降低。各组髁突软骨中ERα、ERβ、OPG和RANKL均有表达,主要分布于肥大软骨层。避孕药组和雷公藤多苷片组ERα、ERβ和OPG表达均降低,RANKL表达均升高。结论血清雌激素浓度降低能够下调大鼠髁突软骨ERα、ERβ和OPG的表达,上调RANKL的表达,降低OPG/RANKL的比例。在血清雌激素降低状态下,雌激素可能通过ER途径调节OPG/RANKL的比例,介导髁突软骨退变及软骨下骨病理改建过程。

关键词: 髁突软骨, 雌激素, 雌激素受体, 骨保护素, 核因子κ, B受体活化因子配体

Abstract: ]PURPOSE: To investigate lowered serum estrogen on the expression of ERα, ERβ, OPG and RANKL of rat condyle and explore the possible mechanisms of idiopathic condylar resorption(ICR). METHODS: Thirty female SD rats were randomly divided into control group, oral contraceptives group and tripterygium glycosides tablets group. The model of lowered serum estrogen level and model of premature ovarian were established by gastric gavage with oral contraceptives pills and tripterygium wilfordii glycosides tablets, respectively. The serum levels of estradiol were detected respectively before experiment and 12 weeks after experiment. After experiment, the expression of ERα, ERβ, OPG and RANKL of condyle were detected by immunohistochemistry technology. SPSS16.0 software package was used for statistical analysis. RESULTS: The serum levels of estradiol were significantly decreased in both experimental groups. ERα, ERβ, OPG and RANKL were expressed in the condylar cartilage in all groups, mainly distributed in the hypertrophic cartilage layer. In oral contraceptives group and tripterygium glycosides tablets group, rat condylar ERα, ERβ and OPG expression were decreased and RANKL expression was increased. CONCLUSIONS: The lowered serum estrogen concentrations decrease the expression of ERα, ERβ and OPG, raise the expression of RANKL of condylar cartilage and reduce the proportion of OPG/RANKL. In the lowered serum levels of estrogen, estrogen may mediate condylar cartilage degeneration and the subchondral bone pathological reconstruction through ER pathway by regulating the ratio of OPG/RANKL.

Key words: Condylar cartilage, Estrogen, Estrogen receptor, Osteoprotegerin, Receptor activator of nuclear factor-κ, B ligand Shanghai J Stomatol, 2015, 24(4):437-441.

中图分类号:

R782.6

李振兴, 陈传俊. 雌激素降低对大鼠髁突软骨ERα、ERβ、OPG和RANKL表达的影响[J]. 上海口腔医学, 2015, 24(4): 437-441.

LI Zhen-xing, CHEN Chuan-jun. The effect of lowered serum levels of estrogen on the expression of ERα, ERβ, OPG and RANKL in rat mandibular condylar cartilage[J]. Shanghai Journal of Stomatology, 2015, 24(4): 437-441.

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