上海口腔医学 ›› 2015, Vol. 24 ›› Issue (2): 129-134.

• 基础研究 •    下一篇

静电纺壳聚糖-聚己内酯纳米纤维膜引导骨再生的体外研究

何莹1,刘昕2,3,唐旭炎1   

  1. 1.安徽医科大学口腔医学院·附属口腔医院,安徽省口腔疾病研究中心实验室,安徽 合肥 230032;
    2.上海交通大学医学院附属第九人民医院,上海 200011;
    3.上海生物材料研究测试中心,上海 200011
  • 收稿日期:2014-10-13 出版日期:2015-04-20 发布日期:2015-07-24
  • 通讯作者: 唐旭炎,E-mail:txy8302@hotmail.com
  • 作者简介:何莹(1988-),女,在读硕士研究生,E-mail:wwhy2013@126.com
  • 基金资助:
    国家自然科学基金(81201191); 安徽医科大学博士科研基金(XJ201108)

In vitro study of electrospun polycaprolactone/chitosan nano fiber membrane guided bone regeneration

HE Ying1,LIU Xin2,3,TANG Xu-yan1   

  1. 1.Stomatologic Hospital & College, Anhui Medical University; Key Lab of Oral Diseases Research of Anhui Province. Hefei 230032, Anhui Province;
    2.Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine. Shanghai 200011;
    3.Shanghai Biological Materials Research and Testing Center. Shanghai 200011, China
  • Received:2014-10-13 Online:2015-04-20 Published:2015-07-24
  • Supported by:
    National Natural Science Foundation of China(81201191) and Doctorate Scientific Research Fund of Anhui Medical University (XJ201108)

摘要: 目的 探讨静电纺壳聚糖(CHS)-聚己内酯(PCL)纳米纤维膜对骨缺损再生作用。方法通过静电纺丝技术制备壳聚糖-聚己内酯纳米纤维膜,以纤维膜作为载体,根据碱性磷酸酶活性和Western 免疫印迹实验结果,探讨纤维膜对细胞成骨分化能力的影响。采用SPSS17.0软件包对数据进行统计学处理。结果静电纺壳聚糖-聚己内酯纳米纤维膜对细胞成骨分化能力的作用显著高于对照组(不含材料组)(P<0.05)。结论静电纺聚己内酯-壳聚糖纳米纤维膜在体外能够促进细胞的成骨分化,为骨缺损及再生修复提供了科学依据。

关键词: 静电纺丝, 壳聚糖, 聚己内酯, 成骨分化

Abstract: PURPOSE: To explore the effect of electrospining chitosan/polycaprolactone nano fiber membrane on regeneration of bone defects in vitro. METHODS: Chitosan/ polycaprolactone nanofiber membrane was prepared by electrospinning technique. According to alkaline phosphatase activity and Western blot results, the ability of osteogenic differentiation of cells by fiber membrane as carrier was evaluated. The data was analyzed statistically with SPSS17.0 software package. RESULTS: The osteogenic differentiation capacity of electrospining chitosan/polycaprolactone nano fiber membrane was significantly higher than the control (without materials), and the difference was significant (P<0.05). CONCLUSIONS: Electrospinning chitosan/polycaprolactone nano fiber membrane can promote osteogenic differentiation of cells in vitro, which provides a scientific basis for clinical regeneration of bone defects.

Key words: Electrospinning, Chitosan, Polycaprolactone, Osteogenic differentiation

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