上皮根鞘结构对牙根发育的影响

上海口腔医学 ›› 2014, Vol. 23 ›› Issue (2): 184-187.

上皮根鞘结构对牙根发育的影响

刘晓辉¹, 苑芳², 邱宜农¹, 李璇¹

1.兰州军区兰州总医院口腔科, 甘肃兰州 730050;
2.中国人民解放军第一医院检验科, 甘肃兰州 730030

收稿日期:2013-05-27 出版日期:2014-04-20 发布日期:2014-05-21
通讯作者: 刘晓辉, Tel:0931-8994692, E-mail:statue0000@126.com
作者简介:刘晓辉(1975-), 男, 博士, 主治医师

The effect of the structure of epithelial root sheath on the development of tooth root

LIU Xiao-hui¹, YUAN Fang², QIU Yi-nong¹, LI Xuan¹

1.Department of Stomatology, the General Hospital of Lanzhou Command. Lanzhou 730050;
2. Department of Clinical Laboratory, the First Hospital of PLA. Lanzhou 730030, Gansu Province, China

Received:2013-05-27 Online:2014-04-20 Published:2014-05-21

摘要/Abstract

摘要： 目的: 研究上皮根鞘的结构对牙根发育的影响。方法: 选择出生后(postnatal, PN)8 d的SD大鼠, 切取第一磨牙牙胚的颈部组织, 用胶原酶和dispase酶消化, 1组直接离心形成细胞团, 另1组加用胰酶消化后, 离心形成细胞团。将细胞团体外培养4 h后, 种植到母鼠肾被膜下, 4周后取材观察。结果: 胶原酶和dispase酶消化可将上皮根鞘解离成碎片, 加用胰酶消化后, 上皮根鞘被解离为单个细胞。含有上皮根鞘碎片的细胞团种植后形成牙根样结构, 而含有上皮根鞘离散细胞的细胞团则形成管状牙本质和骨样牙本质块。结论: 上皮根鞘的结构对其引导牙根发育具有重要作用。

关键词: 上皮根鞘, 牙根, 牙发育

Abstract: PURPOSE: To investigate the effect of the structure of epithelial root sheath (ERS) on the development of tooth root. METHODS: The first molars of postnatal (PN) 8 d SD rats were chosen to harvest the apical tissue. The tissues were digested by the mixture of type I collagenase and dispase. One part of the cell suspension was pelleted by centrifugalization. The other part of the cell suspension was digested by trypsin and then pelleted. The cell pellets were cultured in vitro for 4 hours and then implanted into renal capsule of the mother rats. RESULTS: It was found that ERS was dissociated into fragments by the mixture of type I collagenase and dispase or single cells by trypsin. The pellets containing fragments of ERS generated tooth root-like structure and periodontal tissues. The pellets containing single cells of ERS formed tubular and bone-like dentin. CONCLUSIONS: The bilayered epithelial structure of ERS has important role in the development of tooth root.

Key words: Epithelial root sheath, Tooth root, Tooth development

中图分类号:

R780.2

刘晓辉, 苑芳, 邱宜农, 李璇. 上皮根鞘结构对牙根发育的影响[J]. 上海口腔医学, 2014, 23(2): 184-187.

LIU Xiao-hui, YUAN Fang, QIU Yi-nong, LI Xuan. The effect of the structure of epithelial root sheath on the development of tooth root[J]. Shanghai Journal of Stomatology, 2014, 23(2): 184-187.

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