过量氟对大鼠牙及骨代谢的影响

上海口腔医学 ›› 2014, Vol. 23 ›› Issue (2): 129-132.

• 基础研究 • 下一篇

过量氟对大鼠牙及骨代谢的影响

刘璐, 张颖, 顾何锋, 张凯强, 马林, 程睿波, 张思宇

中国医科大学口腔医学院口腔预防教研室, 辽宁省口腔医学研究所, 辽宁沈阳 110002

收稿日期:2013-08-09 出版日期:2014-04-20 发布日期:2014-05-21
通讯作者: 张颖, zhangyingcmu@vip.163.com
作者简介:刘璐(1982-), 女, 硕士, 主治医师, E-mail:an_lann@163.com
基金资助:
国家自然科学基金(81072245);中国医科大学附属口腔医院青年科研启动基金(K101593-13-42)

The effect of fluoride on the metabolism of teeth and bone in rats

LIU Lu, ZHANG Ying, GU He-feng, ZHANG Kai-qiang, MA Lin, CHENG Rui-bo, ZHANG Si-yu

Department of Preventive Dentistry, School of Stomatology, China Medical University, Liaoning Institute of Dental Research. Shenyang 110002, Liaoning Province, China

Received:2013-08-09 Online:2014-04-20 Published:2014-05-21

摘要/Abstract

摘要： 目的: 观察氟对大鼠牙及骨代谢的影响, 探讨氟斑牙的发病机制。方法: 48只Wistar大鼠随机分为4组, 每组12只。染氟组分别饮用含氟化钠浓度为50、100、150 mg/L的自来水, 对照组饮用常规自来水。8周后麻醉处死, 观察大鼠氟斑牙的发病情况。应用放射免疫法测定血清骨钙素(OC)、甲状旁腺素(PTH)、降钙素(CT)水平;氟离子选择电极法测定血氟及牙氟浓度, 生化方法测定大鼠血清钙含量。采用SPSS13.0软件包对氟斑牙检出率进行χ2检验, 单因素方差分析比较各指标组间差异。结果: 各染氟组大鼠血氟、牙氟含量显著高于对照组(P<0.05), 差异具有显著性, 而且各组氟含量随饮水氟浓度的增高而逐渐增高(F值分别为11.234、275.148, P<0.01)。染氟组大鼠血清钙含量显著低于对照组(P<0.05), 且各组血钙含量随饮水氟浓度的增高而逐渐降低(F=3.906, P<0.05)。中、高剂量组大鼠血清PTH、OC含量显著高于对照组, 差异具有显著性(P<0.01);各组含量随饮水氟浓度的增高而逐渐增高, 组间差异显著(F值分别为8.548、3.801, P<0.05)。低、中剂量组大鼠血清CT呈下降趋势, 但与对照组相比并无显著差异(P>0.05);而高氟剂量组CT比对照组显著下降(P<0.01)。各组血清CT含量的组间差异显著(F=5.121, P<0.05)。结论: 氟影响大鼠的牙及骨代谢, OC、PTH及CT在氟牙症发病中起着重要作用。

关键词: 氟斑牙, 骨钙素, 甲状旁腺素, 降钙素

Abstract: PURPOSE: To investigate the effect of fluoride on the metabolism of teeth and bone in rats, and to probe the mechanism of pathogenesis of dental fluorosis. METHODS: A total of 48 Wistar rats were randomly and equally divided into 4 groups including control group (distilled water), low-dose group(NaF, 50 mg/L), medium-dose group (NaF, 100 mg/L) and high-dose group (NaF, 150 mg/L). After 8 weeks, the rats were sacrificed under anesthesia, and serums were collected. The biochemical technique was used to test serum calcium. Changes in the fluorine content in serums and teeth of each group were analyzed with fluoride ion selective electrode method. Radioimmunoassay was employed to detect the levels of osteocalcin (OC), parathormone (PTH) and calcitonin (CT), respectively. SPSS13.0 software package was used for statistical analysis. RESULTS: The fluorine content in serum and teeth in the fluoride group were significantly higher than that of the control group (P<0.05), and increased with the increasing concentrations (F value was 11..234 and 275.148 respectively, P<0.01). The level of calcium in serum (F=3.906, P<0.05) in the fluoride group was significantly lower than in the control group. The level of PTH and OC in serum in medium and high-dose group were significantly higher than in the control group (P<0.01), with the level of CT in high-dose group decreased significantly (P<0.01). The differences of the level of OC, PTH, CT between groups were significant (F value was 8.548, 3.801 and 5.121 respectively, P<0.05). CONCLUSIONS: Fluoride affects the metabolism of teeth and bone in rats and OC, PTH, CT plays a key role in the pathogenesis of dental fluorosis.

Key words: Dental fluorosis, Osteocalcin, Parathormone, Calcitonin

中图分类号:

R780.1

刘璐, 张颖, 顾何锋, 张凯强, 马林, 程睿波, 张思宇. 过量氟对大鼠牙及骨代谢的影响[J]. 上海口腔医学, 2014, 23(2): 129-132.

LIU Lu, ZHANG Ying, GU He-feng, ZHANG Kai-qiang, MA Lin, CHENG Rui-bo, ZHANG Si-yu. The effect of fluoride on the metabolism of teeth and bone in rats[J]. Shanghai Journal of Stomatology, 2014, 23(2): 129-132.

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过量氟对大鼠牙及骨代谢的影响

The effect of fluoride on the metabolism of teeth and bone in rats

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