雌二醇浓度对大鼠髁突软骨细胞中雌激素受体mRNA表达的影响

上海口腔医学 ›› 2013, Vol. 22 ›› Issue (3): 278-281.

雌二醇浓度对大鼠髁突软骨细胞中雌激素受体mRNA表达的影响

洪露，陈传俊

(安徽医科大学第三附属医院口腔颌面外科，安徽合肥 230061)

收稿日期:2012-03-14 修回日期:2013-01-18 出版日期:2013-06-10 发布日期:2013-06-10
通讯作者: 陈传俊，Tel：0551-2183622，E-mail：ccj6318@sina.com.cn
作者简介:洪露(1986-)女，在读硕士研究生，E-mail:Crazy_ou@Hotmail.com

Effect of estradiol on the expression of estrogen receptor in mandibular condylar cartilage cell of rat

HONG Lu, CHEN Chuan-jun

Department of Oral and Maxillofacial Surgery, the Third Affiliated Hospital of Anhui Medical University. Hefei 230061,Anhui Province,China

Received:2012-03-14 Revised:2013-01-18 Online:2013-06-10 Published:2013-06-10

摘要/Abstract

摘要： 目的：探讨雌二醇(estradiol,E2)对体外培养的髁突软骨细胞雌激素受体(estrogen recerptor,ER)基因表达的影响。方法：体外培养大鼠髁突软骨细胞，分别加入浓度为10-12、10-9、10-6、10-3 mmol/L的17β E2 24 h，实时定量PCR法检测所培养的髁突软骨细胞 ERα、ERβmRNA的表达。采用SPSS10.0软件包对结果进行统计学分析。结果：ERα和ERβ在体外培养的髁突软骨细胞中均有表达，17β E2能够上调ERα的基因表达，同时下调ERβ表达。在10-9 mmol/L的17β E2作用下，上调ERα基因表达最为明显。结论：E2浓度的改变可上调ERα的表达并下调ERβ的表达，在生理范围的雌激素浓度下，E2上调ERα基因表达的作用最为明显。

关键词: 雌激素, 雌激素受体, 髁突软骨细胞, mRNA

Abstract: PURPOSE: To evaluate the effect of estradiol on the expressions of estrogen receptor in mandibular condylar cartilage cell (MCC). METHODS: The MCC were harvested from neonatal rats. The experimental groups was cultured in medium with different concentration(10-3,10-6,10-9,l0-12 mmol/L) of estradiol(E2), while the control group was cultured in medium with ethanol. The ERα mRNA and ERβ mRNA in cells were detected with RT-PCR.The data was processed with SPSS10.0 software package. RESLUTS: Both ERα and ERβ expression were observed in MCC. Exogenous 17β-E2 raised the gene expression of ERα and lowered the expression of ERβ. Under the concentration of 10-9 mmol/L(physiological condition) , the up-regulation of ERα mRNA was found most obviously. CONCLUSIONS: E2 can raise the expression of ERα and decrease the expression of ERβ, and the effect of up grading is the most obvious under physiological concentration.

Key words: Estradiol, Estrogen receptor, Mandibular condylar cartilage cell, mRNA

中图分类号:

P782.6

洪露, 陈传俊. 雌二醇浓度对大鼠髁突软骨细胞中雌激素受体mRNA表达的影响[J]. 上海口腔医学, 2013, 22(3): 278-281.

HONG Lu, CHEN Chuan-jun. Effect of estradiol on the expression of estrogen receptor in mandibular condylar cartilage cell of rat[J]. Shanghai Journal of Stomatology, 2013, 22(3): 278-281.

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