全口义齿修复对口腔微生态影响的初步探讨

doi:10.19439/j.sjos.2018.01.013

上海口腔医学 ›› 2018, Vol. 27 ›› Issue (1): 56-60.doi: 10.19439/j.sjos.2018.01.013

全口义齿修复对口腔微生态影响的初步探讨

于卫强¹, 田菲², 郭晓奎², 张富强¹, 叶冬霞³

1.上海交通大学医学院附属第九人民医院·口腔医学院口腔修复科,上海 200011
2.上海交通大学医学院病原生物学教研室,上海 200025
3.上海市口腔医学研究所,上海 200011

收稿日期:2017-04-13 修回日期:2017-08-15 出版日期:2018-02-25 发布日期:2018-03-05
通讯作者: 叶冬霞,E-mail: yedongxia122@hotmail.com
作者简介:于卫强（1985-）,男,主治医师,E-mail:ywqiang@126.com
基金资助:
上海市卫生局项目 (2012173)

Changes of oral bacteria flora after wearing complete denture

YU Wei-qiang¹, TIAN Fei², GUO Xiao-kui², ZHANG Fu-qiang¹, YE Dong-xia³

1.Department of Prosthodontics, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine. Shanghai 200011
2.Department of Medical Microbiology and Parasitology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine. Shanghai 200025
3. Shanghai Research Institute of Stomatology. Shanghai, 200011, China

Received:2017-04-13 Revised:2017-08-15 Online:2018-02-25 Published:2018-03-05

摘要/Abstract

摘要： 目的: 初步探讨全口义齿修复对口腔微生态的影响。方法: 选取11例全口义齿修复后7 d的患者,收集口腔不同软组织位点、唾液及义齿表面的菌斑,构建细菌16S rRNA基因文库。采用焦磷酸测序技术针对细菌的16S rRNA基因序列的V2-V3可变区进行序列分析,完成口腔菌群的物种鉴定。结果: 11例全口义齿修复患者共测得64800条序列,其中37416条序列为缓症链球菌、溶血孪生球菌、黏液罗氏菌、卟啉单胞菌、动物咬伤奈瑟球菌、苛养颗粒链菌、鲍曼不动杆菌、香茅醇假单胞菌、毗邻颗粒链菌和梭杆菌,为口腔优势菌种。全口义齿组织面的物种与唇颊前庭区和口底菌种较为相似,而全口义齿光滑面的物种与口底及舌腹区黏膜菌种的相似度较高。结论: 全口义齿修复对口腔菌群的影响仍有一定局限性,口腔菌群组成不同还受到个体差异等多因素影响。

关键词: 全口义齿, 口腔微生态, 16S rRNA

Abstract: PURPOSE: This study investigateed the influence of complete denture on oral bacteria flora. METHODS: Bateria plaque samples in oral mucosa, saliva and denture surfaces in 11 edentulous patients were collected, then the 16S rRNA gene clone libraries were constructed. Pyrosequencing was used to analyze the 16S rRNA gene V3~V4 regions, oral bacteria flora were classified and identified. RESULTS: There were 64800 sequences in complete denture-wearing subjects, Streptococcus mitis, Gemella haemolysans, Rothia mucilaginosa, Porphyromonas sp, Neisseria zoodegmatis, Granulicatella elegans, Acinetobacter baumannii, Pseudomonas citrinum, Granulicatella adiacens and Fusobacterium canifelinum were the predominant species (37416 sequences). The species of denture tissue surface were similar to these of buccal vestibule after wearing denture, and the species of denture smooth surface were similar to these of tongue ventrum and the floor of mouth. CONCLUSIONS: The effect of complete denture on oral flora is still limited, and the composition of oral flora is influenced by many other factors.

Key words: Complete denture, Oral bacteria flora, 16S rRNA

中图分类号:

R783

于卫强, 田菲, 郭晓奎, 张富强, 叶冬霞. 全口义齿修复对口腔微生态影响的初步探讨[J]. 上海口腔医学, 2018, 27(1): 56-60.

YU Wei-qiang, TIAN Fei, GUO Xiao-kui, ZHANG Fu-qiang, YE Dong-xia. Changes of oral bacteria flora after wearing complete denture[J]. Shanghai Journal of Stomatology, 2018, 27(1): 56-60.

参考文献

[1] Xu J.Microbial ecology in the age of genomics and metagenomics: concepts, tools, and recent advances[J]. Mol Ecol, 2006, 15(7): 1713-1731.
[2] 张松梅, 田非, 黄庆丰, 等. 基于16基因序列分析1例健康人龈上菌群[J]. 中国微生态学杂志, 2011, 23(1): 44-48.
[3] Huang XL, Exterkate RA, ten Cate JM. Factors associated with alkali production from arginine in dental biofilms[J]. J Dent Res, 2012, 91(12): 1130-1134.
[4] Zhang SM, Tian F, Huang QF, et al.Bacterial diversity of subgingival plaque in 6 healthy Chinese individuals[J]. Exp Ther Med, 2011, 2(5): 1023-1029.
[5] Zhang SM, Tian F, Jiang X, et al.Evidence for calcifying nanoparticles in gingival crevicular fluid and dental calculus in periodontitis[J]. J Periodontol, 2009, 80(9): 1462-1470.
[6] Keijser BJ, Zaura E, Huse SM, et al.Pyrosequencing analysis of the oral microflora of healthy adults[J]. J Dent Res, 2008, 87(11): 1016-1020.
[7] Yu W, Qian C, Zhang S, et al.Effects of lipopolysaccharides on the corrosion behavior of Ni-Cr and Co-Cr alloys[J]. J Prosthet Dent, 2016, 116(2): 286-291.
[8] Zhang S, Qiu J, Ren Y, et al.Reciprocal interaction between dental alloy biocorrosion and Streptococcus mutans virulent gene expression[J]. J Mater Sci Mater Med, 2016, 27(4):78
[9] Aas JA, Paster BJ, Stokes LN, et al.Defining the normal bacterial flora of the oral cavity[J]. J Clin Microbiol, 2005, 43(11): 5721-5732.
[10] 张松梅, 田菲, 李静, 等. 对纳米细菌在牙结石形成过程中的作用的初步研究[J]. 中国微生态学杂志, 2009, 21(2): 152-155.
[11] Campos MS, Marchini L, Bernardes LA, et al.Biofilm microbial communities of denture stomatitis[J]. Oral Microbiol Immunol, 2008, 23(5): 419-424.
[12] Zhang SM, Qiu J, Tian F, et al.Corrosion behavior of pure titanium in the presence of Actinomyces naeslundii[J]. J Mater Sci Mater Med, 2013, 24(5): 1229-1237.
[13] Huang XL, Schulte RM, Burne RA, et al.Characterization of the arginolytic microflora provides insights into ph homeostasis in human oral biofilms[J]. Caries Res, 2015, 49(2): 165-176.
[14] Zhao X, Pan J, Zhang S, et al.Effectiveness of resin-based materials against erosive and abrasive enamel wear[J]. Clin Oral Investig, 2017, 21(1): 463-468.
[15] Zhou J, Huang Q, Wang X, et al.Early loading of splinted implants in the posterior mandible: a prospective multicentre case series[J]. J Clin Periodontol, 2016, 43(3): 298-304.
[16] 张松梅. 基于16S rRNA基因序列对口腔菌群组成的初步研究[D]. 上海: 上海交通大学: 2009.
[17] 熊薇, 张文池. 鲍曼不动杆菌的耐药性[J]. 中国抗感染化疗杂志, 2001, 1(2): 96-97.
[18] Sachdeo A, Haffajee AD, Socransky SS.Biofilms in the edentulous oral cavity[J]. J Prosthodont, 2008, 17(5): 348-356.
[19] Zhao YF, Zeng DL, Zhang SM, et al.Osteogenic potential of bone marrow stromal cells derived from streptozotocin-induced diabetic rats[J]. Int J Mol Med, 2013, 31(3): 614-620.
[20] Hope CK, Wilson M.Biofilm structure and cell vitality in a laboratory model of subgingival plaque[J]. J Microbiol Methods, 2006, 66(3): 390-398.
[21] Zhu X, Wang S, Gu Y, et al.Possible variation of the human oral bacterial community after wearing removable partial dentures by DGGE[J]. World J Microbiol Biotechnol, 2012, 28(5): 2229-2236.

全口义齿修复对口腔微生态影响的初步探讨

Changes of oral bacteria flora after wearing complete denture

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 10

编辑推荐

Metrics

本文评价

[1]	周团锋, 杨雪, 张华, 张智勇, 陈全. 48例Locator种植覆盖全口义齿5年临床修复效果评价[J]. 上海口腔医学, 2024, 33(1): 90-96.
[2]	俞灏, 郭晓静, 钟群, 王珏. 二次功能印模法全口义齿对改善无牙颌患者口腔健康的效果评价[J]. 上海口腔医学, 2023, 32(4): 401-404.
[3]	邓钰玮, 文晋, 赵军, 程蕙娟. 56例成人牙列缺失患者生物功能性全口义齿治疗的满意度及修复效果评价[J]. 上海口腔医学, 2021, 30(3): 328-331.
[4]	郭天文, 邢鹤琳, 王菁, 王宝成. 先天性缺牙患儿可摘义齿修复61例总结[J]. 上海口腔医学, 2019, 28(2): 196-200.
[5]	李伟宏, 黄睿, 林华, 李庆艳, 郑新颖, 吕俏, 高宁. 铍对牙龈卟啉单胞菌细胞形态和细胞膜离子含量的影响[J]. 上海口腔医学, 2015, 24(4): 385-389.
[6]	胡晓萍,朱洪水,喻静雯. 全口义齿垂直距离恢复过低对脑血流的影响[J]. 上海口腔医学, 2015, 24(1): 111-114.
[7]	钟群, 彭艳, 邬雪颖, 夏萌培. 义齿稳固剂影响无牙下颌牙槽嵴应力分布的光弹性分析[J]. 上海口腔医学, 2014, 23(1): 46-50.
[8]	焦纪兰, 曾利伟, 周年苟, 邓璐, 陈萍. 义齿黏附剂和口腔湿润剂联合使用提高口干症患者全口义齿满意度的临床评价[J]. 上海口腔医学, 2013, 22(5): 585-588.
[9]	马春敏;沈庆平;钟群;戴文安;沈晴昳 . 舌向集中?咀嚼效能的评价 [J]. 上海口腔医学, 2012, 21(4): 470-473.
[10]	祁兵;罗卫;张景林. 降低垂直距离在老年全口义齿修复中的应用 [J]. 上海口腔医学, 2012, 21(3): 337-339.