不同放疗剂量对人唾液腺腺样囊性癌细胞生物学行为的影响

上海口腔医学 ›› 2016, Vol. 25 ›› Issue (4): 403-408.

不同放疗剂量对人唾液腺腺样囊性癌细胞生物学行为的影响

黄育萌^{1, 2}, 王颖³, 李勇¹, 刘平¹, 柳先锋³, 赵洪伟^{1, 4}

1.重庆医科大学附属口腔医院口腔颌面外科,重庆 401147; 2.口腔疾病与生物医学重庆市重点实验室,重庆 401147; 3.重庆市肿瘤医院肿瘤放射治疗科,重庆 400030; 4.重庆市高校市级口腔生物医学工程重点实验室,重庆 401147

收稿日期:2016-01-29 出版日期:2016-08-25 发布日期:2016-09-06
通讯作者: 赵洪伟,E-mail:bradfordwei@163.com
作者简介:黄育萌(1988-),男,硕士研究生,E-mail:huangymcq@163.com
基金资助:
重庆市医学科研计划(2015ZDXM019)

Effect of different doses of radiation on human salivary gland adenoid cystic carcinoma cells

HUANG Yu-meng^{1, 2}, WANG Ying³, LI Yong¹, LIU Ping¹, LIU Xian-feng³, ZHAO Hong-wei^{1, 4}

1.Department of Oral and Maxillofacial Surgery, Stomatological Hospital of Chongqing Medical University. Chongqing 401147;
2.Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences. Chongqing 401147;
3.Department of Tumor Radiotherapy of Chongqing Tumor Hospital. Chongqing 400030;
4. Colleges and Universities in Chongqing Municipal Oral Key Laboratory of Biomedical Engineering. Chongqing 401147, China

Received:2016-01-29 Online:2016-08-25 Published:2016-09-06

摘要/Abstract

摘要： 目的探讨不同放疗剂量对人唾液腺腺样囊性癌（SACC-83、SACC-LM）细胞生物学行为的影响。方法采用不同放疗剂量（0、2、4、6、8、10 Gy）作用SACC-83、SACC-LM细胞并继续培养48 h,CCK-8检测细胞存活,流式细胞仪检测细胞凋亡和细胞周期,细胞划痕实验观察细胞迁移能力变化。采用SPSS19.0软件包对数据进行统计学分析。结果放疗对SACC-LM细胞的细胞存活率、细胞凋亡率、异倍体、细胞迁移力的影响较SACC-83显著(P<0.05);与其他放疗剂量相比,SACC-83细胞在6 Gy剂量照射下细胞存活率最低,细胞凋亡比例及异倍体比例最高,细胞迁移能力最弱。结论SACC-LM细胞放疗敏感性较SACC-83更高,SACC-83细胞对6 Gy剂量放疗最为敏感。

关键词: 唾液腺, 腺样囊性癌, 放疗敏感性, 细胞凋亡

Abstract: PURPOSE: To explore the effect of different doses of radiation on human salivary gland adenoid cystic carcinoma cells (SACC-83, SACC-LM). METHODS: Different doses of radiation (0, 2, 4, 6, 8, 10 Gy) were applied to SACC-83, SACC-LM cells and the cells were continued to culture for 48 h. CCK-8 test, flow cytometry(FCM) and cell scratch experiment were used to observe cell proliferation, apoptosis and cell migration. SPSS19.0 software package was used for data analysis. RESULTS: The effect of radiation on SACC-LM cells survival rate, cell apoptosis, heteroploid and cell migration ability were significantly greater than that on SACC-83 cells (P<0.05). In SACC-83 cells, compared with other doses of radiation, 6 Gy of irradiation was the most sensitive on cell survival rate, cell apoptosis, heteroploid and cell migration ability. CONCLUSIONS: Radiation sensitivity of SACC-LM was greater than that of SACC-83 cells. With 6 Gy of radiation, changes of biology in SACC-83 cells most significant than other doses of radiation.

Key words: Salivary gland, Adenoid cystic carcinoma, Radiation sensitivity, Cell apoptosis.

中图分类号:

R730.261

黄育萌, 王颖, 李勇, 刘平, 柳先锋, 赵洪伟. 不同放疗剂量对人唾液腺腺样囊性癌细胞生物学行为的影响[J]. 上海口腔医学, 2016, 25(4): 403-408.

HUANG Yu-meng, WANG Ying, LI Yong, LIU Ping, LIU Xian-feng, ZHAO Hong-wei. Effect of different doses of radiation on human salivary gland adenoid cystic carcinoma cells[J]. Shanghai Journal of Stomatology, 2016, 25(4): 403-408.

参考文献

[1] Lee SY, Kim BH, Choi EC. Nineteen-year oncologic outcomes and the benefit of elective neck dissection in salivary gland adenoid cystic carcinoma [J].Head Neck, 2014, 36(12): 1796-1801.
[2] Balamucki CJ, Amdur RJ, Werning JW, et al. Adenoid cystic carcinoma of the head and neck[J]. Am J Otolaryngol,2012,33(5): 510-508.
[3] Shigeishi H, Ohta K, Okui G, et al. Clinicopathological analysis of salivary gland carcinomas and literature review[J].Mol Clin Oncol, 2015, 3(1): 202-206.
[4] Li N, Xu L, Zhao H, et al. A comparison of the demographics, clinical features, and survival of patients with adenoid cystic carcinoma of major and minor salivary glands versus less common sites within the surveillance, epidemiology, and end results registry [J].Cancer, 2012, 118(16): 3945-3953.
[5] Garden AS, Weber RS, Ang KK, et al. Postoperative radiation therapy for malignant tumors of minor salivary glands. Outcome and patterns of failure [J].Cancer, 1994, 73(10):2563-2569.
[6] Zeidan YH, Shultz DB, Murphy JD, et al. Long-term outcomes of surgery followed by radiation therapy for minor salivary gland carcinomas [J].Laryngoscope, 2013, 123(11): 2675-2680.
[7] Chen AM, Bucci MK, Weinberg V, et al. Adenoid cystic carcinoma of the head and neck treated by surgery with or without postoperative radiation therapy: prognostic features of recurrence [J]. Int J Radiat Oncol Biol Phys, 2006, 66(1): 152-159.
[8] Chummun S, Mclean NR, Kelly CG, et al. Adenoid cystic carcinoma of the head and neck [J]. Br J Plast Surg, 2001, 54(6): 476-480.
[9] 陈智贤, 孙爱民, 陈勇, 等. 放射敏感性、X线辐射剂量对鼻咽癌细胞miR-7表达的影响 [J].南方医科大学学报, 2010, 30(8): 1810-1812, 1816.
[10] Chang B, Li S, He Q, et al. Deregulation of Bmi-1 is associated with enhanced migration, invasion and poor prognosis in salivary adenoid cystic carcinoma[J]. Biochim Biophys Acta, 2014, 1840 (12): 3285-3291.
[11] Jeong WJ, Choi IJ, Park MW, et al. CXCR4 antagonist inhibits perineural invasion of adenoid cystic carcinoma [J]. J Clin Pathol, 2014, 67(11): 992-998.
[12] Zhou XQ, Huang SY, Zhang DS, et al. Effects of 5-aza-2'deoxycytidine on RECK gene expression and tumor invasion in salivary adenoid cystic carcinoma [J]. Braz J Med Biol Res, 2015, 48(3):254-260.
[13] 张瑞智, 张萍, 余波, 等. Cyr61对人腺样囊性癌细胞系生物学特性的影响 [J]. 实用口腔医学杂志, 2014, 30(4): 526-529.
[14] Ren J, Chu Y, Ma H, et al. Epigenetic interventions increase the radiation sensitivity of cancer cells [J]. Curr Pharm Des, 2014, 20(11):1857-1865.
[15] Li LQ, Li XL, Wang L, et al. Matrine inhibits breast cancer growth via miR-21/PTEN/Akt pathway in MCF-7 cells [J]. Cell Physiol Biochem, 2012, 30(3):631-641.
[16] 刘浏, 刘秀明, 吕燕, 等. MAB225对人唾液腺腺样囊性癌细胞放射敏感性的影响 [J]. 上海口腔医学, 2011, 20(6): 603-607.