Fe2+、F-对釉质抗酸性的增强作用及机制

上海口腔医学 ›› 2016, Vol. 25 ›› Issue (1): 42-46.

Fe2+、F-对釉质抗酸性的增强作用及机制

张月¹,²，黄瑞哲¹,²

1.西安交通大学口腔医学院陕西省颅颌面精准医学研究重点实验室，陕西省牙颌面疾病临床研究中心，陕西西安 710004；
2.西安交通大学口腔医院预防科，陕西西安 710004

收稿日期:2015-03-18 修回日期:2015-05-20 出版日期:2016-02-25 发布日期:2016-03-09
通讯作者: 黄瑞哲，Tel:029-87219383,E-mail:hrzh8897@sina.com E-mail:zhangyue198717@sina.com
作者简介:张月（1986-），女，硕士，住院医师

Study on enhancement effect and mechanism of the acid resistance of enamel by Fe2+ and F-

ZHANG-Yue¹,², HUANG Rui-zhe¹,².

1.Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, Clinical Research Center of Shannxi Province for Dental and Maxillofacial Diseases, College of Stomatology, Xi’an Jiaotong University. Xi’an 710004;
2.Department of Preventive Dentistry, School of Stomatology, Xi’an Jiaotong University. Xi’an 710004, Shaanxi Province, China

Received:2015-03-18 Revised:2015-05-20 Online:2016-02-25 Published:2016-03-09

摘要/Abstract

摘要： 目的： 通过X线衍射技术分析硫酸亚铁、氟化钠处理釉粉后形成新物质的物相及对釉质晶格的影响，以探讨Fe2+、F-增强釉质抗酸性的作用机制。方法： 收集磨牙50颗，切取釉质并研磨成粉末共10 g，平均分为4组：C组（对照组，去离子水）、Fe2+组（15 mmol/L FeSO4）、F-组（1.23% NaF）、Fe2+ + F-组（15 mmol/L FeSO4 + 1.23% NaF）。将样本在各组实验溶液中孵育48 h，然后进行脱矿-再矿化循环，共6次。1个完整周期包括：① 在5 mL可口可乐（pH=2.58）中脱矿5 min；② 在37℃ 5 mL人工唾液中再矿化1 h。将釉粉在40℃下烘干，进行X射线衍射分析。结果： XRD定性分析显示，硫酸亚铁溶液使X衍射谱线半宽高增宽、变平缓。釉质晶粒尺寸减小，结晶度降低。次要相检索显示有新物相磷酸铁生成；铁氟联合作用与氟化物单独作用衍射谱线相似，使衍射峰半高宽变窄、变尖锐，提高了晶体的结晶度。次要相检索有氟化钙生成。结论： Fe2+、F-均对釉质晶体结构有一定影响。高浓度F-和Fe2+联合作用时Fe2+的作用被掩盖。Fe2+可能通过替换Ca2+而使釉质晶粒减小，结晶度降低；同时，Fe2+和釉质表面溶解的PO43-结合，形成磷酸铁抗酸沉淀；F-通过替换OH-，参与釉质再矿化，使釉质结晶度增加，同时在釉质表面形成氟化钙沉淀，抵抗釉质酸蚀。

关键词: 硫酸亚铁, 牙釉质, 抗酸性, X衍射

Abstract: PURPOSE: The phase of new substances and the effect on enamel lattice was analysed after ferrous sulfate ,sodium fluoride treatment through X-ray diffraction, in order to explore the mechanism of Fe2+ and F- reinforced enamel acid resistance. METHODS： Fragments of enamel obtained from fifty molars were ground into powder and randomly divided into four groups: C (control,deionized water); Fe2+ (15 mmol/L FeSO4); F- (1.23% NaF) and Fe2+ + F- (15 mmol/L FeSO4 and 1.23% NaF ). Before exposure to acid, the samples were incubated in one of the experimental solutions for 48 hours. After that, the samples were submitted to six alternating remineralization and demineralization cycles.A complete cycle consisted of the following steps:① demineralization in 5 mL of the beverage (Coca-Cola, pH=2.58) for 5 minutes under gentle agitation;② remineralization in 5 mL of artificial saliva for 1 hour at 37℃. X-ray diffraction was employed to identify precipitates and estimate their lattice constants before enamel power dried at 40℃. RESULTS: The XRD pattern of the group of ferrous sulfate was not sharper than control while the full width half maximum of peak increased and became more gentle. The enamel crystal grain size and crystallinity decreased. Secondary phase retrieval showed there was new phase formed which was iron phosphate. Diffraction spectrum of ferrous sulfate with fluoride group was similar to fluoride alone, the full width half maximum of peak became sharper and more narrow.The enamel crystal grain crystallinity improved and there was new phase of calcium fluoride formed. CONCLUSIONS: Fe2+ and F- both had certain influence on the crystal structure of enamel. However, the impact of Fe2+ was concealed when reacted with high concentration F-. Fe2+ may participate in the nucleation of apatite through substitution of calcium in apatite. Acid-resistant enamel surfaces are established due to precipitation of ferric phosphates on the enamel surface, and combination of Fe2+ with PO43- dissolved on enamel surface.F- may participate in the nucleation of apatite through substitution of OH-; at the same time, the calcium fluoride precipitation was established.

Key words: Ferrous sulfate, Enamel, Acid resistance, X-ray diffraction

中图分类号:

R780.1

张月,黄瑞哲,. Fe2+、F-对釉质抗酸性的增强作用及机制[J]. 上海口腔医学, 2016, 25(1): 42-46.

ZHANG-Yue,².. Study on enhancement effect and mechanism of the acid resistance of enamel by Fe2+ and F-[J]. Shanghai Journal of Stomatology, 2016, 25(1): 42-46.

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