氢氧化钙对根尖周成骨细胞IL-6、TNF-α表达的影响

上海口腔医学 ›› 2016, Vol. 25 ›› Issue (1): 32-37.

氢氧化钙对根尖周成骨细胞IL-6、TNF-α表达的影响

孟令娇，仇丽鸿，于雅琼，郭佳杰，詹福良，张凌，张晓芳

中国医科大学口腔医学院牙体牙髓病科；
辽宁省口腔医学研究所；
辽宁省口腔疾病重点实验室辽宁省口腔疾病转化医学研究中心，辽宁沈阳 110002

收稿日期:2015-02-10 修回日期:2015-05-16 出版日期:2016-02-25 发布日期:2016-03-09
通讯作者: 仇丽鸿，E-mail: drqlh@yahoo.com E-mail:yjswz999@163.com
作者简介:孟令娇 (1988-)，女，硕士，住院医师
基金资助:
辽宁省自然科学基金（2014021055）

The effect of calcium hydroxide on IL-6 and TNF-α expression of osteoblast in periapical tissues

MENG Ling-jiao, QIU Li-hong, YU Ya-qiong, GUO Jia-jie, ZHAN Fu-liang, ZHANG Ling, ZHANG Xiao-fang.

Department of Endodontics, School of Stomatology, China Medical University;
Liaoning Institute of Dental Research;
Liaoning Key Laboratory of Oral Diseases & Research Center of Oral Disease Translational Medicine. Shenyang 110002, Liaoning Province, China

Received:2015-02-10 Revised:2015-05-16 Online:2016-02-25 Published:2016-03-09

摘要/Abstract

摘要： 目的： 检测氢氧化钙置于根管不同位置根尖周pH值变化及牙髓卟啉单胞菌（Porphynomonasl endodontics，P.e）刺激成骨细胞IL-6、TNF-α表达的影响。方法： 选取140颗无菌人单根管离体牙，根管预备后随机分为6个实验组(每组20个样本)和1个对照组 (20个样本)。1~3组分别将手调氢氧化钙糊剂封入根管下1/2、根管上1/2和髓室，4~6组将Apexcal封入根管下1/2、根管上1/2和髓室，对照组不封药。每组随机选取10颗离体牙置于P.e悬液中，于封药后3、7 d测量各组根尖周区细菌对MC3T3-E1细胞IL-6、TNF-α表达的影响。每组剩余10颗离体牙置于蒸馏水中，检测封药后3、7、14、21 d根尖周pH值变化。采用SPSS 13.0软件包对数据进行统计学分析。结果： 2种氢氧化钙封入根管不同位置后，随着时间延长，根尖周pH值升高；14 d时达到高峰。手调氢氧化钙糊剂充填髓室组根尖周pH值升高程度与其他实验组相比有显著差异，根尖周P.e菌液预处理的MC3T3-E1细胞IL-6、TNF-α表达量较对照组显著降低，各实验组间IL-6、TNF-α表达量无显著差异。结论： 氢氧化钙糊剂封入根管不同部位均使根尖周pH升高，抑制根尖周P.e刺激成骨细胞表达IL-6、TNF-α。

关键词: 氢氧化钙, pH, 牙髓卟啉单胞菌, 成骨细胞, 炎症因子

Abstract: PURPOSE: To compare the effect of calcium hydroxide in different position on pH and inflammation factor expression of periapical osteoblasts. METHODS： 140 sterilized single-rooted human teeth models were randomly divided into 6 experiment groups and one control group: Group 1-3:calcium hydroxide paste was placed in the apical half of root canal, the upper half of root canal and the pulp champer; Group 4-6:Apexcal was placed in the apical half of root canal, the upper half of root canal and the pulp champer; Group 7: the control group without medication. 10 teeth of each group were placed in P.e suspension, the IL-6 and TNF-α expression of MC3T3-E1 was tested at 3 d and 7 d. The other teeth of each group were placed in distilled water, and the pH in periapical region was tested at 3, 7, 14 and 21 d. SPSS 13.0 software package was used for statistical analysis. RESULTS: Calcium hydroxide placed in different position of the root canal increased periapical pH value and reached its peak at 14 d. The group in which calcium hydroxide paste was placed in pulp chamber gained lower pH level than other experimental groups. IL-6, TNF-α expression of MC3T3-E1 pretreated by P.e suspension of experimental groups was significantly reduced compared with control group, and there was no significant difference between the experimental groups. CONCLUSIONS: Calcium hydroxide placed in different position of the root canal could increase periapical pH value and reduce IL - 6, TNF-α expression of periapical osteoblasts.

Key words: Calcium hydroxide, pH, Porphynomonasl endodontics, Osteoblast, Inflammation factor

中图分类号:

R781.3

孟令娇，仇丽鸿，于雅琼，郭佳杰，詹福良，张凌，张晓芳. 氢氧化钙对根尖周成骨细胞IL-6、TNF-α表达的影响[J]. 上海口腔医学, 2016, 25(1): 32-37.

MENG Ling-jiao, QIU Li-hong, YU Ya-qiong, GUO Jia-jie, ZHAN Fu-liang, ZHANG Ling, ZHANG Xiao-fang.. The effect of calcium hydroxide on IL-6 and TNF-α expression of osteoblast in periapical tissues[J]. Shanghai Journal of Stomatology, 2016, 25(1): 32-37.

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