不同浓度SDF1、bFGF、IL-8对骨髓间充质干细胞的趋化作用

上海口腔医学 ›› 2015, Vol. 24 ›› Issue (3): 275-279.

不同浓度SDF1、bFGF、IL-8对骨髓间充质干细胞的趋化作用

刘玉健,汪俊^*,池政兵^*

上海交通大学医学院附属第九人民医院·口腔医学院儿童口腔科,上海市口腔医学重点实验室,上海 200011

收稿日期:2014-09-30 出版日期:2015-06-20 发布日期:2015-07-24
通讯作者: 汪俊,E-mail:wangjun202@126.com;池政兵,E-mail:zbchi120966@sina.com。
作者简介:刘玉健(1989-)女,硕士,E-mail:yujianyuexing@126.com
基金资助:
上海市科学技术委员会医学引导项目（124119b0101）

Comparison of chemotaxis of different concentrations of SDF1,bFGF,IL-8 on bone marrow mesenchymal stem cell

LIU Yu-jian,WANG Jun,CHI Zheng-bin

Department of Pediatric Dentistry, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine;Shanghai Key Laboratory of Stomatology. Shanghai 200011, China

Received:2014-09-30 Online:2015-06-20 Published:2015-07-24
Supported by:
Medical Guide Project of Science and Technology Committee of Shanghai Municipality（124119b0101）

摘要/Abstract

摘要： 目的:研究比较不同趋化因子[骨髓基质细胞衍生因子1（stromal cell-derived factor-1,SDF-1）、碱性成纤维细胞生长因子（basic fibroblast growth factor,bFGF）和白介素8（interleukin-8,IL-8）]在多个浓度下对骨髓间充质干细胞（bone marrow mesenchymal stem cells,BMSCs）的趋化作用。方法:实验分为3组,即SDF1组、bFGF组、IL-8组,每组因子浓度为0 （对照浓度）、10、50、100、200、400 ng/mL（实验浓度）。抽取SD大鼠骨髓,分离培养BMSCs,利用Transwell小室研究不同浓度SDF1、bFGF、IL-8对BMSCs的趋化作用,获得3种因子趋化BMSCs的最佳浓度。采用SPSS16.0软件包对数据进行统计学分析。结果:与0 ng/mL（对照浓度）组相比,3种因子均可趋化BMSCs（P<0.05）; 50、100、400 ng/mL浓度下,SDF1对BMSCs的趋化作用显著高于bFGF（P<0.05）;200 ng/mL浓度下,SDF1对BMSCs的趋化作用强于IL-8;SDF1及IL-8对BMSCc趋化作用的最佳浓度均为100 ng/mL,bFGF的最佳浓度为200 ng/mL。结论:SDF-1、bFGF、IL-8均可趋化BMSCs,SDF-1与IL-8的趋化效果更佳。

关键词: SDF1, bFGF, IL-8, 骨髓间充质干细胞, 趋化作用

Abstract: PURPOSE: To investigate chemotaxis of different concentrations of chemokines stromal cell-derived factor-1 (SDF-1), basic fibroblast growth factor (bFGF) and interleukin-8 (IL-8）on bone marrow mesenchymal stem cells (BMSCs). METHODS: The experiment group was divided into 3 groups according to different culture media: SDF1, bFGF and IL-8. The chemokine concentrations of each group was 0 ng/mL (control concentration),10 ng/mL, 50 ng/mL, 100 ng/mL, 200ng/mL and 400 ng/mL (experimental concentration). BMSCs were isolated from SD rats and cultured in vitro. 24-well plates with an 8 μm pore size polycarbonate membrane were used to examine the chemotaxis of SDF1, bFGF, IL-8 on BMSCs, respectively, and to obtain the best chemotactic concentrations of 3 chemokines. The data was analyzed with SPSS 16.0 software package. RESULTS: Compared with 0 ng/mL group, all chemokines could chemoattract BMSCs (P<0.05). At the concentration of 50 ng/mL, 100 ng/mL and 400 ng/mL, SDF1 significantly recruited more cells than bFGF (P<0.05); SDF1 significantly recruited more cells than IL-8 at the concentration of 200 ng/mL; The best chemotactic concentration was 100 ng/mL for SDF1 and IL-8 and 200 ng/mL for bFGF. CONCLUSIONS: The use of SDF1,bFGF,IL-8 can chemoattract BMSCs. The chemotaxis of SDF1 and IL-8 are better than bFGF.

Key words: SDF1, BFGF, IL-8, BMSCs, Chemotaxis

中图分类号:

R780.2

刘玉健,汪俊,池政兵. 不同浓度SDF1、bFGF、IL-8对骨髓间充质干细胞的趋化作用[J]. 上海口腔医学, 2015, 24(3): 275-279.

LIU Yu-jian,WANG Jun,CHI Zheng-bin. Comparison of chemotaxis of different concentrations of SDF1,bFGF,IL-8 on bone marrow mesenchymal stem cell[J]. Shanghai Journal of Stomatology, 2015, 24(3): 275-279.

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