DEK原癌基因与口腔鳞状细胞癌的关系

上海口腔医学 ›› 2014, Vol. 23 ›› Issue (1): 75-79.

DEK原癌基因与口腔鳞状细胞癌的关系

王璇¹, 黄绍辉¹, 於丽乔¹, 刘洁², 刁尧², 孙长伏¹

1.中国医科大学口腔医学院口腔颌面外科,辽宁省口腔医学研究所,辽宁沈阳 110002;
2.中国医科大学实验技术中心二部,辽宁沈阳 110002

收稿日期:2013-05-17 修回日期:2013-07-14 出版日期:2014-02-20 发布日期:2014-10-21
通讯作者: 黄绍辉,Tel:024-22891022,E-mail: huangshaohui@hotmail.com
作者简介:王璇(1986-),女,在读硕士研究生,E-mail: wangxuanstepanie@163.com

Relationship between DEK oncogene expression and oral squamous cell carcinoma

WANG Xuan¹, HUANG Shao-hui¹, YU Li-qiao¹, LIU Jie², DIAO Yao², SUN Chang-fu¹

1.Department of Oral and Maxillofacial Surgery, School of Stomatology, China Medical University, Liaoning Research Institute of Stomatology. Shenyang 110002, Liaoning Province, China;
2.Department of Central Laboratory, China Medical University.Shenyang 110002, Liaoning Province, China

Received:2013-05-17 Revised:2013-07-14 Online:2014-02-20 Published:2014-10-21

摘要/Abstract

摘要： 目的：研究原癌基因DEK在口腔鳞状细胞癌组织中的表达及意义,探讨DEK在口腔鳞状细胞癌发病及转移机制中的作用。方法：采用反转录聚合酶链反应 (RT-PCR)检测33例口腔鳞状细胞癌病灶组织、28例口腔鳞癌转移淋巴结组织和33例癌旁组织中DEK mRNA的表达,并用免疫组织化学法检测DEK蛋白在3种组织中的表达,应用SPSS17.0软件包对结果进行统计学分析。结果：DEK mRNA及DEK蛋白在口腔鳞状细胞癌转移淋巴结(82.1%、82.1%)和口腔鳞状细胞癌病灶组织(81.2%、76.8%)中的阳性表达率均高于癌旁组织(27.3%、18.2%),且DEK mRNA及DEK蛋白在病灶组织和癌旁组织中的表达差异均具有显著性(P<0.05)。结论：DEK基因在人口腔鳞状细胞癌的发生、发展中起着重要作用。

关键词: DEK, 口腔鳞状细胞癌, 细胞凋亡

Abstract: PURPOSE: To investigate the expression of gene DEK in oral squamous cell carcinoma, and explore the role of DEK in the development and metastasis of oral squamous cell carcinoma. METHODS: Thirty-three specimens of oral squamous cell carcinoma (OSCC), 28 specimens of cervical lymph nodes metastasis, and 33 specimens of normal oral mucosal tissues adjacent to the cancerous lesions were examined. Polymerase chain reaction with reverse transcription (RT - PCR) and immunohistochemistry were used to detect mRNA and protein expression of DEK, respectively.The results were analysed with SPSS17.0 software package. RESULTS: RT-PCR and immunohistochemistry showed that the expression of DEK mRNA and DEK protein in metastatic lymph nodes (82.1%, 82.1%) and cancerous tissues (81.2%, 76.8%) of OSCC were significantly higher than in the adjacent tissues (27.3%, 18.2%). The difference between cancerous tissues and adjacent tissues was significant (P<0.05). CONCLUSION: DEK may play an important role in the occurrence and metastasis of OSCC.

Key words: DEK, Oral squamous cell carcinoma, Cell apoptosis

中图分类号:

R739.8

王璇, 黄绍辉, 於丽乔, 刘洁, 刁尧, 孙长伏. DEK原癌基因与口腔鳞状细胞癌的关系[J]. 上海口腔医学, 2014, 23(1): 75-79.

WANG Xuan, HUANG Shao-hui, YU Li-qiao, LIU Jie, DIAO Yao, SUN Chang-fu. Relationship between DEK oncogene expression and oral squamous cell carcinoma[J]. Shanghai Journal of Stomatology, 2014, 23(1): 75-79.

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