低糖基化E-cadherins对舌鳞癌细胞增殖和侵袭的影响

上海口腔医学 ›› 2014, Vol. 23 ›› Issue (1): 1-6.

• 基础研究 • 下一篇

低糖基化E-cadherins对舌鳞癌细胞增殖和侵袭的影响

张平平¹, 徐秀英¹, 高振南^{1, 2}

1.山东大学口腔医学院,山东济南 250012;
2.山东省口腔生物医学重点实验室,山东济南 250012

收稿日期:2013-05-31 修回日期:2013-07-31 出版日期:2014-02-20 发布日期:2014-10-21
通讯作者: 高振南,Tel：0531-88382851,E-mail: zngao@sdu.edu.cn
作者简介:张平平(1984-),女,在读硕士研究生,E-mail: zpplm@126.com
基金资助:
教育部第40批留学回国人员科研启动基金项目; 山东省优秀中青年科学家科研奖励基金(BS2010YY021)

Effect of hypoglycosylated E-cadherins on proliferation and invasiveness of tongue squamous cell carcinoma

ZHANG Ping-ping1¹, XU Xiu-ying1¹, GAO Zhen-nan1^{1, 2}

1.School of Stomatology, Shandong University. Jinan 250012;
2.Shandong Provincial Key Laboratory of Oral Biomedicine. Jinan 250012, Shandong Province, China

Received:2013-05-31 Revised:2013-07-31 Online:2014-02-20 Published:2014-10-21
Supported by:
Scientific Research Foundation for the Returned Oversea Chinese Scholars; State Ministry of Education of China and Shandong Provincial Award Foundation for Excellent Middle- and Young-Aged Scientists (BS2010YY021)

摘要/Abstract

摘要： 目的：研究低糖基化E-cadherins对舌鳞癌细胞CAL 27增殖和侵袭的影响,并探讨E-cadherins低糖基化修饰对其介导的细胞间黏附连接(adherens junctions,AJs)稳定性的影响。方法：用编码有FLAG标签的低糖基化E-cadherins和野生型E-cadherins质粒分别转染CAL 27细胞。48 h后,用抗FLAG抗体作为一抗,Western免疫印迹检测外源性E-cadherins的表达;用细胞增殖计数、单层细胞划痕愈合和细胞体外侵袭实验检测细胞的增殖和侵袭能力;免疫沉淀法检测E-cadherins介导的AJs复合体中的α-catenins、β-catenins、γ-catenins和vinculins的含量。采用SPSS17.0软件包对数据进行统计学分析。结果：被转染细胞均有外源性E-cadherins表达。被转染细胞的增殖和侵袭能力比未被转染对照细胞显著减弱(P<0.05),而且低糖基化E-cadherins对CAL 27细胞增殖和侵袭能力的抑制作用比野生型E-cadherins显著增强(P<0.05)。低糖基化E-cadherins介导的AJs复合体中的α-catenins、β-catenins、γ-catenins和vinculins的含量均比野生型E-cadherins介导的AJs复合体显著增多(P<0.01)。结论：低糖基化E-cadherins比野生型E-cadherins更加显著抑制舌鳞癌细胞的增殖和侵袭,其机制是低糖基化E-cadherins介导的AJs比野生型E-cadherins介导的AJs更加稳定。

关键词: 糖基化, E-cadherin, 舌鳞癌, 细胞增殖, 肿瘤侵袭, 黏附连接

Abstract: PURPOSE: To investigate the effect of N-glycosylated E-cadherins on proliferation and invasiveness of tongue squamous cell carcinoma CAL 27 cells, and to study the effect of hypoglycosylation of E-cadherins on the stability of adherens junctions (AJs) mediated by E-cadherins. METHODS: Human tongue squamous cell carcinoma CAL 27 cells were respectively transfected with the plasmids encoding either hypoglycosylated or wild-type E-cadherins with FLAG as tags. Western blot of FLAG was used to detect the expression of exogenous E-cadherins in the transfected cells after 48 hours. The cell proliferation counting, monolayer cell scratch wound healing and in vitro cell invasiveness assays were performed to evaluate the proliferation and invasiveness of cells. Immunoprecipitation experiments were used to assay the quantity of α-catenins, β-catenins, γ-catenins and vinculins linked with the cytosolic tails of E-cadherins. All statistical analysis were performed using SPSS 17.0 software package. RESULTS: The expression of exogenous E-cadherins was detected in transfected CAL 27 cells by Western blot. Cell proliferation counting assay revealed that hypoglycosylated E-cadherins significantly inhibited proliferation of CAL 27 cells, compared with wild-type E-cadherins. The monolayer cell scratch wound healing and in vitro cell invasiveness assays indicated that hypoglycosylated E-cadherins also significantly restrained both invasiveness of CAL 27 cells, compared with wild-type E-cadherins. The immunoprecipitation experiments demonstrated that hypoglycosylated E-cadherins exhibited an increased association with α-catenins, β-catenins, and γ-catenins and vinculins. CONCLUSIONS: Compared with wild-type E-cadherins, hypoglycosylated E-cadherins can significantly suppress proliferation and invasiveness of tongue squamous cell carcinoma CAL 27 cells, and the mechanism was that hypoglycosylated E-cadherins can induce more stable AJs than wild-type E-cadherin.

Key words: Glycosylation, E-cadherin, Tongue squamous cell carcinoma neoplasms, Cell proliferation, Neoplasm invasiveness, Adherens junctions

中图分类号:

R739.8

张平平, 徐秀英, 高振南. 低糖基化E-cadherins对舌鳞癌细胞增殖和侵袭的影响[J]. 上海口腔医学, 2014, 23(1): 1-6.

ZHANG Ping-ping1, XU Xiu-ying1, GAO Zhen-nan1. Effect of hypoglycosylated E-cadherins on proliferation and invasiveness of tongue squamous cell carcinoma[J]. Shanghai Journal of Stomatology, 2014, 23(1): 1-6.

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低糖基化E-cadherins对舌鳞癌细胞增殖和侵袭的影响

Effect of hypoglycosylated E-cadherins on proliferation and invasiveness of tongue squamous cell carcinoma

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