上海口腔医学

上海口腔医学 ›› 2018, Vol. 27 ›› Issue (2): 164-169.doi: 10.19439/j.sjos.2018.02.010

• 论著 • 上一篇    下一篇

WNT10A基因rs10177996位点单核苷酸多态性在新疆地区维族和汉族人群中的分布

刘佳林1, 赵春萍2, 张嘉宇1, 韩祥祯1, 葛树鹏1, 杜丽娟1, 何惠宇1   

  1. 1.新疆医科大学第一附属医院 口腔修复科,新疆 乌鲁木齐 830054;
    2.新疆喀什地区第一人民医院 口腔科,新疆 喀什 844000
  • 收稿日期:2017-09-05 修回日期:2017-11-15 出版日期:2018-04-25 发布日期:2018-05-14
  • 通讯作者: 何惠宇,E-mail: hehuiyu01@126.com
  • 作者简介:刘佳林（1992-）,女,硕士研究生,E-mail: 476039114@qq.com
  • 基金资助:
    新疆维吾尔自治区科技支疆项目（201591191）

Distribution of WNT10A gene rs10177996 polymorphism between Han and Uygur populations in Xinjiang area

LIU Jia-lin1, ZHAO Chun-ping2, ZHANG Jia-yu1, HAN Xiang-zhen1, GE Shu-peng1, DU Li-juan1, HE Hui-yu1   

  1. 1.Department of Prosthodontics, the First Affiliated Hospital of Xinjiang Medical University. Urumqi 830054;
    2.Department of Prosthodontics, the First People's Hospital of Kashgar Area. Kashgar 844000, Xinjiang Uygur Autonomous Region, China
  • Received:2017-09-05 Revised:2017-11-15 Online:2018-04-25 Published:2018-05-14

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摘要： 目的:研究新疆地区维吾尔族和汉族人群中WNT10A基因rs10177996位点单核苷酸多态性的分布特征。方法:采用横断面调查,以新疆乌鲁木齐地区154例汉族个体及新疆喀什地区134例维吾尔族个体为研究对象,采用棉拭子法获取颊黏膜脱落的上皮细胞,用专用试剂盒提取DNA并检测,利用PCR（酶合聚链反应）技术扩增合格样品中含有的相应SNP的序列片段,基因测序后,结合软件进行基因分型。采用SPSS 23.0软件包对所得数据进行统计学分析。结果:新疆喀什地区维吾尔族人群中WNT10A基因rs10177996位点CC、CT和TT的基因型频率分别为8.21%、30.60%和61.19% ,C/T等位基因频率分别为C=23.51%,T=76.49%。新疆乌鲁木齐地区汉族人群中WNT10A基因rs10177996位点CC、CT和TT的基因型频率分别为9.74%、43.51%和46.75%,C/T等位基因频率分别为C=31.49%,T=68.51%。新疆喀什地区维吾尔族人群与新疆乌鲁木齐地区汉族人群相比,TT型基因型频率分布高,CC型分布低,差异具有显著性（P=0.046）。新疆喀什地区维吾尔族人群与欧洲人群相比,维吾尔族人群中SNP位点的TT型基因型频率分布低,CC型分布高,差异具有显著性（P=0.050）。新疆乌鲁木齐地区汉族人群与欧洲人群相比,TT型基因型频率分布低,CC型分布高,差异具有显著性（P<0.01）。在上述3种人群中,关于C/T等位基因频率的分布,新疆乌鲁木齐地区汉族人群与欧洲人群相比,C等位基因频率的分布高,T等位基因频率的分布低,差异具有显著性（P=0.033）。但新疆喀什地区维吾尔族人群与新疆乌鲁木齐地区汉族人群比较,以及新疆喀什地区维吾尔族人群与欧洲人群比较,均无显著差异（P>0.05）。新疆乌鲁木齐地区汉族人群和新疆喀什地区维吾尔族人群不同性别间也无显著差异（P>0.05）。结论:在新疆乌鲁木齐地区汉族人群、新疆喀什地区维吾尔族人群以及已报道的欧洲人群中,WNT10A基因rs10177996位点单核苷酸多态性分布特征各不相同。

关键词: WNT10A基因, 单核苷酸多态性（SNP）, 维吾尔族, 汉族

Abstract: PURPOSE: To explore the characteristics of distribution of WNT10A gene rs10177996 polymorphism between Han and Uygur populations in Xinjiang area. METHODS: A cross-sectional survey on 154 Han individuals in Urumqi area and 134 Uygur individuals in Kashgar area was performed. Buccal epithelial cells were harvested using Cotton swab scraping, and DNA was extracted by special kit. After screening, the corresponding SNP segments of qualified samples were propagated by PCR. Dideoxy-mediated chain termination method was used for gene sequencing, and then, genotyping was conducted with corresponding software. Statistical analysis of genetic data was performed by SPSS 23.0 software package. RESULTS: Among Uygur nationality in Kashgar area, the frequencies of CC, CT, TT genetypes in rs10177996 were 8.21%, 30.60% and 61.19%, respectively. The allele frequency of C was 23.51% and T was 76.49%. Among Han nationality in Urumqi area, the frequencies on CC, CT, TT genetypes of rs10177996 were 9.74%, 43.51% and 46.75%, respectively. The allele frequency of C was 31.49% and T was 68.51%. When compared with Han nationality, the frequency of TT was significantly higher in Uygur nationality(P=0.046). When compared with European, the frequency of TT was significantly lower in Uygur nationality (P=0.05). When compared with European, the frequency of TT was significantly lower in Han nationality(P<0.01). Compared with European, the distribution on C allele frequency was significantly higher, the distribution on T allele frequency was significantly lower in Han nationality (P=0.033). However, there was no significant difference between Han nationality in Urumqi area and Uygur nationality in Kashgar area (P>0.05), and, between Uygur nationality in Kashgar area and European (P>0.05). Meanwhile, there was no significant difference in gender between Uygur nationality in Kashgar area and Han nationality in Urumqi area (P>0.05). CONCLUSIONS: The distributions of WNT10A gene rs10177996 SNP among Han nationality in Urumqi area, Uygur nationality in Kashgar area and the reported European population are obviously different.

Key words: WNT10A gene, Single nucleonic polymorphisms, Uygur nationality, Han nationality

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