Abstract: PURPOSE: To investigate the effect of IL-10 gene polymorphism on periodontal microecology in patients with periodontitis. METHODS: Seventy-nine patients with periodontitis (experimental group) and 50 healthy individuals (control group) visiting Zibo First Hospital from June 2018 to March 2019 were enrolled. The experimental group was further divided into two groups according to the severity of periodontitis, namely moderate periodontitis (n=47) and severe periodontitis (n=32). Real-time quantitative PCR was used to detect the level of bacterial colonization in the subgingival plaque of all subjects. The genotype and allele frequencies at-1082,-819, and-592 loci in IL-10 were detected by PCR. Multivariate analysis was used to explore the relationship between IL-10 genotypes and bacterial colonization level in subgingival plaque. The data were processed by SPSS 2.0 software package. RESULTS: Actinomycetes actinomycetes (A.a) colonization in patients with moderate periodontitis was significantly lower than that of patients with severe periodontitis (P<0.05). The amount of Porphyromonas gingivalis(P.g) and A.a in patients with moderate periodontitis and severe periodontitis was significantly higher than that in the control group (P<0.05). The proportion of CC genotypes in the-819 locus of patients with moderate periodontitis was significantly lower than that of the control group, and the proportion of TT genotype was significantly higher than that of the control group(P<0.05). The proportion of CC, TC and TT genotypes showed no significant difference between patients with moderate periodontitis and those with severe periodontitis (P>0.05). The proportion of CC genotypes in the-592 locus of patients with moderate periodontitis was significantly lower than that of the control group, and the proportion of AA genotype was significantly higher than that of the control group (P<0.05). In moderate periodontitis, A.a colonization amount at-592 gene locus of AA genotype was significantly higher than that of AC genotype(P<0.05). In moderate periodontitis, A.a colonization amount at of-819 gene locus of TT genotype was significantly higher than that of TC genotype (P<0.05). Multivariate analysis showed that IL-10-592-AA genotype and-819-TT genotype were closely related to A.a colonization in moderate periodontitis (P<0.05). CONCLUSIONS: IL-10 gene polymorphism is the influencing factor of subgingival microecological environment in patients with moderate periodontitis, among which IL-10-592-AA genotype and-819-TT genotype have the most obvious correlation with A.a colonization level.

Key words: IL-10 gene polymorphism, Periodontitis, Periodontal microecology