MicroRNA-125b regulates osteogenic differentiation of human periodontal ligament stem cells

doi:10.19439/j.sjos.2018.01.003

Abstract

Abstract: PURPOSE: To investigate the effect of microRNA-125b (miR-125b) on osteogenic differentiation of periodontal ligament stem cell (PDLSCs). METHODS: The surface factor of isolated PDLSCs was detected by flow cytometry. After transfected with miR-125b or anti-miR125b in PDLSCs, MTT and LDH were used to detect cell viability and cytotoxicity; ALP activity and calcium level were detected by ALP assay kit and calcium colorimetric assay kit. Western blot and RT-qPCR were used to determine the expression of osteogenesis-related genes. The interaction between miR-125b and connexin 43 (Cx43) was detected by dual luciferase gene reporter assay. The data were analyzed with SPSS 17.0 software. RESULTS: The cultured PDLSCs showed the characteristics of mesenchymal stem cells. After transfected with miR-125b in PDLSCs, the cell viability was decreased, cytotoxicity was increased; ALP activity, calcium level and the expression of osteogenesis-related genes were significantly decreased. On the contrary, cell viability, ALP activity, calcium level and the expression of osteogenesis-related genes in PDLSCs were increased after anti-miR-125b transfection. Dual luciferase gene reporter assay showed that miR-125b could target Cx43. In addition, the effect of miR-125b on osteogenic differentiation of PDLSCs was reversed after Cx43 overexpression. CONCLUSIONS: miR-125b may inhibit osteogenic differentiation of PDLSCs by targeting Cx43.

Key words: Periodontal ligament stem cell, MicroRNA-125b, Connexin43

CLC Number:

R781.4

DU Li, CAO Wei-jing, TIAN Ying, WANG Yuan-ming. MicroRNA-125b regulates osteogenic differentiation of human periodontal ligament stem cells[J]. Shanghai Journal of Stomatology, 2018, 27(1): 11-17.

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