Abstract: PURPOSE:This experiment was aimed at exploring whether carboxymethyl chitosan zinc and peptide (CMC-Zn+-P) can reduce the occurrence and development of periodontal tissue inflammation effectively by observing the change of IL-1,TNF-α and PGE-2 level in gingival crevicular fluid (GCF) before and after brushing, so as to find a new effective material in preventing and treating periodontal diseases. METHODS:Miniature pigs were selected as experimental subjects and divided into 4 groups randomly: the control group; CMC-Zn+-P group (material group);brushing group; brushing + CMC-Zn+-P group (composite group). Gingival crevicular fluid before and one month after the experiment was collected. The levels of IL-1, TNF-α and PGE-2 were examined by enzyme-linked immune-sorbent assay, while the clinical periodontal index was recorded. SPSS 18.0 software package was used for statistical analysis. RESULTS:There was no significant difference in levels of IL-1, TNF-α and PGE-2 and clinical periodontal index between the 4 groups before experiment. After one month, the levels of IL-1, TNF-α, PGE-2 in GCF had significant difference between 4 groups. The levels of IL-1, TNF-α, PGE-2 in composite group were significant lower than that of the other three groups (P<0.008).The levels of IL-1, TNF-αand PGE-2 in the material group and brushing group were significantly lower than that of the control group (P<0.008). Compared with materials group, the brushing group had significantly lower level of IL-1,significantly higher level of PGE-2 ,but no difference in the level of TNF-α.In addition, the teeth calculus index of composite group was significantly lower than that of other groups (P<0.05). CONCLUSIONS: CMC-Zn+-P can effectively reduce periodontal tissue inflammation and cut down the speed of deposition of dental calculus. If used cooperatively with brushing, the effect will be better.

Key words: Miniature pigs, Carboxymethyl chitosan zinc and peptide, IL-1, TNF-α, PGE-2