Effects of PRF and released three growth factors on migration of rat adipose tissue-derived stem cells

Abstract

Abstract: PURPOSE To analyze the effects of PRF and released three growth factors on migration of rat adipose tissue-derived stem cells and to investigate the mechanism of migration. METHODS The inguinal adipose tissue of rat was excised at aseptic condition to obtain primary ADSCs by enzyme digestion. Multi-directional differentiation was used to identify the ADSCs. PRF membrane was acquired through one time centrifuge. The cell migration was examined by Transwell assay and wound healing assay. The mRNA expression of MMP2 and MT1-MMP was tested by real-time PCR. Statistical analysis was performed using SPSS 13.0 software package. RESULTS Cell migration test showed that the migration of rat ADSCs in PRF group were significantly higher than those in the negative group（P＜0.05） and inhibitor group（P＜0.05）. The ADSCs migration effects in three growth factors group at different concentrations showed significant difference（P＜0.05）. Real-time PCR showed that gene expressions of MMP2 and MT1-MMP were significantly higher in PRF group than control group (P＜0.05). PCR showed that gene expressions of MMP2 and MT1-MMP were significantly higher in three growth factors group than control group (P＜0.05). CONCLUTIONS: PRF and three growth factors consistently enhanced the migration of rat ADSCs in a dose-response manner. The migration increase of rat ADSCs may be associated with the up-regulation of MMP2 and MT1-MMP gene expression.

Key words: PRF, ADSCs, Migration, TGF-β1, PDGF-AB, VEGF

CLC Number:

R782

Cite this article

GAO Jie, WANG Ming-guo, YANG Shuai, LI Xiu-mei, YANG Shi-mao, LI Xue.. Effects of PRF and released three growth factors on migration of rat adipose tissue-derived stem cells[J]. Shanghai Journal of Stomatology, 2015, 24(6): 667-673.

share this article

/ Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://shkqyx.magtechjournal.com/EN/

https://shkqyx.magtechjournal.com/EN/Y2015/V24/I6/667

References

[1] Zuk PA, Zhu M, Ashjian P, et al. Human adipose tissue is a source of multipotent stem cells [J]. Mol Biol Cell, 2002, 13(12): 4279-4295.
[2] Choukroun J, Adda F, Schoeffler C, et al. Une opportunitéen paro-implantologie: Le PRF[J]. Implantodontie, 2001, 42: 55-62.
[3] Dohan Ehrenfest DM, de Peppo GM, Doglioli P, et al. Slow release of growth facto-rs and thrombospondin-1 in Choukroun’s platelet-rich fibrin (PRF): a gold standard t-o achieve for all surgical platelet concentrates tehnologies [J]. Growth Factors, 2009,27(1): 63-69.
[4] Lee JW, Kim SG, Kim JY, et al. Restoration of a peri-implant defect by platelet-richfibrin [J]. Oral Surg Oral Med Oral Pathol Oral Radiol, 2012, 113(4): 459-463.
[5] Toffler M, Toscano N, Holtzclaw D. Osteotome-mediated sinus floor elevation using only platelet-rich fibrin: an early report on 110 patients [J]. Implant Dent, 2010, 19(5): 447-456.
[6] Peck MT, Marnewick J, Stephen L. Alveolar ridge preservation using leukocyte and platelet-rich fibrin: a report of a case [J].Case Rep Dent, 2011, 2011: 345048.
[7] Chen FM, Wu LA, Zhang M, et al. Homing of endogenous stem/progenitor cells for in situ tissue regeneration: Promises, strategies, and translational perspectives [J]. Biomaterials, 2011, 32(12): 3189-3209.
[8] 杨世茂,王明国, 李静. 富血小板纤维蛋白与富血小板血浆体外释放生长因子的比较及其对脂肪干细胞增殖分化的影响 [J]. 华西口腔医学杂志, 2012, 30(6): 641-644.
[9] Ji B, Sheng L, Chen G, et al. The combination use of platelet-rich fibrin and treated dentin matrix for tooth root regeneration by cell homing [J]. Tissue Eng Part A, 2015,21(1-2): 26-34.
[10] 范文娟, 杨明, 张晨, 等. 富血小板纤维蛋白对人牙龈成纤维细胞增殖、迁移和分泌Ι型胶原的影响 [J]. 中华口腔医学杂志, 2013, 48(2): 72-76.
[11] Chang IC, Tsai CH, Chang YC. Platelet-rich fibrin modulates the expression of extr-acellular signal-regulated protein kinase and osteoprotegerin in human osteoblasts [J]. J Biomed Mater Res A, 2010, 95(1): 327-332.
[12] Tang J, Wang J, Kong X, et al. Vascular endothelial growth factor promotes cardiac stem cell migration via the PI3K/Akt pathway [J]. Exp Cell Res, 2009, 315(20): 3521-3531.
[13] Ries C, Egea V, Karow M, et al. MMP-2, MT1-MMP, and TIMP-2 are essential for the invasive capacity of human mesenchymal stem cells: differential regulation by inflammatory cytokines [J]. Blood, 2007, 109(9): 4055-4063.
[14] Cho A, Reidy MA. Matrix metalloproteinase-9 is necessary for the regulation of smooth muscle cell replication and migration after arterial injury [J]. Circ Res, 2002,91(9): 845-851.
[15] Matter CM, Rozenberg I, Jaschko A, et al. Effects of tacrolimus or sirolimus on proliferation of vascular smooth muscle and endothelial cells [J]. J Cardiovasc Pharmacol, 2006, 48(6): 286-292.
[16] Kamath AT, Rochat AF, Valenti MP, et al. Adult-like anti-mycobacterial T cell and in vivo dendritic cell responses following neonatal immunization with Ag85B-ESAT-6 in the IC31 adjuvant [J]. PloS One, 2008, 3(11): e3683.

Related Articles 15

[1] LIU Wen-jing, LI Meng-qi, CUI Xiang, WANG Jun-lan. Study on the molecular mechanism of circ_0000326 regulating the proliferation, invasion and migration of oral squamous cell carcinoma HSC3 cells by targeting miR-567 [J]. Shanghai Journal of Stomatology, 2023, 32(6): 590-596.

[2] LIU Yun, CHEN Hao-yue, MI Jing, WANG Fei-fei, WAN Guang-yong, CUI Chao. Inhibitory effect of paeoniflorin on CAL27 cells of tongue squamous cell carcinoma and its mechanism [J]. Shanghai Journal of Stomatology, 2023, 32(6): 597-602.

[3] XU Fang-fang, JIANG Bei-zhan. Effect of DKK1 on the biological behaviors of dental pulp cells exposed to lipopolysaccharide [J]. Shanghai Journal of Stomatology, 2021, 30(4): 355-359.

[4] WANG Zhen, SUN Yang, CHEN Jia-lu, GONG Yi-ming. Effect of curcumin on TGF-β1 /Smad3 pathway in rat gingival fibroblast treated with cyclosporine A [J]. Shanghai Journal of Stomatology, 2020, 29(4): 359-364.

[5] LI Huai-qi, YE Jin-hai, DING Xu, WU He-ming. Effect of mi-138 targeting PLD2 gene on proliferation and migration of oral cancer cells [J]. Shanghai Journal of Stomatology, 2020, 29(1): 25-30.

[6] FANG Shou-rong. Role of Bcl-2 and MIF proteins in senile patients with gingival cancer [J]. Shanghai Journal of Stomatology, 2020, 29(1): 105-108.

[7] ZHAO Xian, ZENG Jin, ZUO Dong-chuan, XU Jie, YU Jing-hong, XU Xiao-mei. Effect of calcium on proliferation, migration and osteogenic differentiation of human dental follicle cells [J]. Shanghai Journal of Stomatology, 2019, 28(6): 572-577.

[8] LANG Miao-jie, OU Yue-jian. The influence of zoledronic acid on proliferation, migration and apoptosis of fibroblasts [J]. Shanghai Journal of Stomatology, 2019, 28(5): 490-493.

[9] LIN Zhe-jing, LUO Ren-hui, XIE Li-li, WU Xiao-yu. Effects of basic periodontal treatment on endothelin, vascular endothelial growth factor-A and tumor necrosis factor-α levels in gingival crevicular fluid and serum [J]. Shanghai Journal of Stomatology, 2019, 28(5): 504-508.

[10] KANG Jian-yong, HE Juan, DUAN Xiao-feng. Biological effect of nicotine on Cal27 cell line in patients with tongue squamous cell carcinoma [J]. Shanghai Journal of Stomatology, 2019, 28(4): 368-372.

[11] WANG Jing, FANGTENG Jiao-zi, LIU He. Effect of iRoot BP Plus on biological behavior of deciduous tooth pulp stem cells and human pulp stem cells [J]. Shanghai Journal of Stomatology, 2019, 28(3): 251-258.

[12] WANG De-fang, LIU Yan, WANG Jue. rhPDGF-BB promotes migration of bone marrow stromal stem cells in diabetic rats [J]. Shanghai Journal of Stomatology, 2018, 27(6): 579-584.

[13] SUN Yuan-qing, LIANG Bing, ZHANG Lei, XU Hui. Effects of sevoflurane on the migration of vascular endothelial cells [J]. Shanghai Journal of Stomatology, 2017, 26(3): 272-276.

[14] CHENG Hui-juan, HUA Wei-lei, XU Kan. Occlusal adjustment treatment of pathological migration of the anterior teeth: report of one case [J]. Shanghai Journal of Stomatology, 2016, 25(5): 630-632.

[15] XU Gao-li,WANG Shu-hua,SHI Qiu-tao,LIU Yi,YAN Fan,XU Ting,GU Zhi-yuan. The expression of VEGF-C in the condylar cartilage after anterior disc displacement and its clinical significance [J]. Shanghai Journal of Stomatology, 2016, 25(2): 136-140.

Recommended Articles

Metrics

Viewed

Full text

Abstract

Comments

Abstract

References

Related Articles

Recommended Articles

Metrics

Comments

TOP

Effects of PRF and released three growth factors on migration of rat adipose tissue-derived stem cells

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LIU Wen-jing, LI Meng-qi, CUI Xiang, WANG Jun-lan. Study on the molecular mechanism of circ_0000326 regulating the proliferation, invasion and migration of oral squamous cell carcinoma HSC3 cells by targeting miR-567 [J]. Shanghai Journal of Stomatology, 2023, 32(6): 590-596.
[2]	LIU Yun, CHEN Hao-yue, MI Jing, WANG Fei-fei, WAN Guang-yong, CUI Chao. Inhibitory effect of paeoniflorin on CAL27 cells of tongue squamous cell carcinoma and its mechanism [J]. Shanghai Journal of Stomatology, 2023, 32(6): 597-602.
[3]	XU Fang-fang, JIANG Bei-zhan. Effect of DKK1 on the biological behaviors of dental pulp cells exposed to lipopolysaccharide [J]. Shanghai Journal of Stomatology, 2021, 30(4): 355-359.
[4]	WANG Zhen, SUN Yang, CHEN Jia-lu, GONG Yi-ming. Effect of curcumin on TGF-β1 /Smad3 pathway in rat gingival fibroblast treated with cyclosporine A [J]. Shanghai Journal of Stomatology, 2020, 29(4): 359-364.
[5]	LI Huai-qi, YE Jin-hai, DING Xu, WU He-ming. Effect of mi-138 targeting PLD2 gene on proliferation and migration of oral cancer cells [J]. Shanghai Journal of Stomatology, 2020, 29(1): 25-30.
[6]	FANG Shou-rong. Role of Bcl-2 and MIF proteins in senile patients with gingival cancer [J]. Shanghai Journal of Stomatology, 2020, 29(1): 105-108.
[7]	ZHAO Xian, ZENG Jin, ZUO Dong-chuan, XU Jie, YU Jing-hong, XU Xiao-mei. Effect of calcium on proliferation, migration and osteogenic differentiation of human dental follicle cells [J]. Shanghai Journal of Stomatology, 2019, 28(6): 572-577.
[8]	LANG Miao-jie, OU Yue-jian. The influence of zoledronic acid on proliferation, migration and apoptosis of fibroblasts [J]. Shanghai Journal of Stomatology, 2019, 28(5): 490-493.
[9]	LIN Zhe-jing, LUO Ren-hui, XIE Li-li, WU Xiao-yu. Effects of basic periodontal treatment on endothelin, vascular endothelial growth factor-A and tumor necrosis factor-α levels in gingival crevicular fluid and serum [J]. Shanghai Journal of Stomatology, 2019, 28(5): 504-508.
[10]	KANG Jian-yong, HE Juan, DUAN Xiao-feng. Biological effect of nicotine on Cal27 cell line in patients with tongue squamous cell carcinoma [J]. Shanghai Journal of Stomatology, 2019, 28(4): 368-372.
[11]	WANG Jing, FANGTENG Jiao-zi, LIU He. Effect of iRoot BP Plus on biological behavior of deciduous tooth pulp stem cells and human pulp stem cells [J]. Shanghai Journal of Stomatology, 2019, 28(3): 251-258.
[12]	WANG De-fang, LIU Yan, WANG Jue. rhPDGF-BB promotes migration of bone marrow stromal stem cells in diabetic rats [J]. Shanghai Journal of Stomatology, 2018, 27(6): 579-584.
[13]	SUN Yuan-qing, LIANG Bing, ZHANG Lei, XU Hui. Effects of sevoflurane on the migration of vascular endothelial cells [J]. Shanghai Journal of Stomatology, 2017, 26(3): 272-276.
[14]	CHENG Hui-juan, HUA Wei-lei, XU Kan. Occlusal adjustment treatment of pathological migration of the anterior teeth: report of one case [J]. Shanghai Journal of Stomatology, 2016, 25(5): 630-632.
[15]	XU Gao-li,WANG Shu-hua,SHI Qiu-tao,LIU Yi,YAN Fan,XU Ting,GU Zhi-yuan. The expression of VEGF-C in the condylar cartilage after anterior disc displacement and its clinical significance [J]. Shanghai Journal of Stomatology, 2016, 25(2): 136-140.