Flow cytometry analysis and eIF4E expression of para-tumor tissues of oral squamous cell carcinoma

Abstract

Abstract: PURPOSE:To explore the surgical safe margin of oral squamous cell carcinoma (OSCC), the expression of eIF4E, DNA content and ploidy of para-tumor tissues were analyzed using immunohistochemistry and flow cytometry. METHODS: The specimens were embedded, cut and separated into 5 regions with the labels of T (center of the tumor), P₁ (0-0.5 cm to tumor center), P₂ (0.5-1 cm to tumor center), P₃ (1-1.5 cm to tumor center), and P₄(1.5-2 cm to tumor center). Twenty normal oral mucosal specimens were selected as control and labeled as N. Immunohistochemistry was employed to detect the expression of eIF4E. Moreover, flow cytometer (FCM) was used to assess the DNA content and type. The data was analyzed with SPSS13.0 software package. RESULTS: Ratio of heteroploidy, SPF and PI dropped gradually with the increment of distance of regions to tumor center. But, statistical differences were still observed between para-tumor tissues and normal tissues. eIF4E decreased from center to outer regions but still had 30% of significantly positive expression at P₄ region. CONCLUSIONS: Ratio of heteroploidy, SPF, PI and the expression of eIF4E dropped gradually with the increment of distance of regions to tumor center. Significant difference could still be noticed at the region 2 cm away from tumor tissues, which indicated that if possible 2 cm clinical boundary should be ensured during operation. Otherwise, post-operational chemical-and radio-therapies should be provided.

Key words: Oral squamous cell carcinoma, Para-tumor tissue, eIF4E, Flow cytometry

XU Bo, CHEN Jin-qiang, RAN Hong-bing. Flow cytometry analysis and eIF4E expression of para-tumor tissues of oral squamous cell carcinoma[J]. Shanghai Journal of Stomatology, 2015, 24(5): 446-451.

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