Shanghai Journal of Stomatology

Shanghai Journal of Stomatology ›› 2015, Vol. 24 ›› Issue (5): 405-409.

• Basic Research • Previous Articles     Next Articles

Expression of long non-coding RNA MALAT1 gene in tongue squamous cell carcinoma and cell lines and its biological significance after knockdown the expression of MALAT1

ZHANG Shan-shan1, 2, YANG Hong-yu1, WANG Yu-fan1, YANG Hui-jun1, SHEN Shi-yue1, WANG Feng1, XIE Shu-le1, 2, JIN Long1   

  1. 1.Department of Oral and Maxillofacial Surgery, Peking University Shenzhen Hospital. Shenzhen 518036;
    2.Shantou University Medical College. Shantou 515041, Guangdong Province, China
  • Received:2015-01-26 Revised:2015-03-26 Online:2015-10-20 Published:2015-11-03

Abstract: PURPOSE:To explore the expression of MALAT1 in tongue squamous cell carcinoma and cell lines and its biological significance after knockdown the expression of MALAT1. METHODS: Real-time PCR was used to detect the expression of MALAT1 in tongue squamous cell carcinoma and cell lines SCC9, SCC15, SCC25 and CAL27. The expression of MALAT1 was knocked down by the lentivirus expression vectors GV248 built by biological company in 4 cell lines, which were handed over to another biological company for analysis by gene chip with GraphPad. Prism.v5.0 software. RESULTS: Compared with the control group, the expression of MALAT1 was significantly higher in tongue squamous cell carcinoma tissues and 4 cell lines. After transfection, the expression of MALAT1 was shown more than 75% decrease in CAL27 and SCC25 cell lines. The expression of apoptosis associated genes BNIP3L, NRG1 was down regulated. CONCLUSIONS: MALAT1 gene can be a marker as tumor and regulate the expression of apoptosis associated genes BNIP3L, NRG1, which can be a potential target for treatment of tongue squamous cell carcinoma.