A electron microscope study of bone tissue in mandibular osteoradionecrosis

Abstract

Abstract: PURPOSE:After establishing the animal model, electron microscope was used to observe the dynamic ultrastructural changes during the process of mandibular osteoradionecrosis (ORN) for investigation of the possible pathogenesis. METHODS: A single dose of irradiation (IR25Gy, 28Gy) was delivered via a electronic liner accelerator to right mandible of 6 miniature pigs by utilizing the 3D-CRT technique. Samples of sequestrations were extracted at 3, 4 and 5 months after irradiation for electron microscopic analysis. RESULTS: Osteocollagenous fibers were destroyed shortly after irradiation. Cellular membrane was destroyed at early stage. Then vacuole formed in the cytoplasm and organelle began to split. At last, nucleus disaggregated. CONCLUSIONS: Doses of irradiation are closely related to degree of sequestration. Cellular membrane and cytoplasm are more sensitive to the irradiation than cellular nucleus which is different from the previous findings.

Key words: Supported, by, National, Natural, Science, Foundation, of, China, (81170935).

LI Song, FANG Dian-ji, WANG Zhan-yi, ZHAI Lu-lu, NING Zhao-rong, GUO Yan-wei. A electron microscope study of bone tissue in mandibular osteoradionecrosis[J]. Shanghai Journal of Stomatology, 2015, 24(5): 395-399.

References

[1] Lyons A, Osher J, Wamer E, et al. Osteoradionecrosis-a review current concepts in defining the extent of the disease and a new classification proposal [J]. Br J Oral Maxillofac Surg, 2014, 52(5): 392-395.
[2] Jereczek-Fossa BA, Orecchia R. Radiotherapy-induced mandibular bone complications[J]. Cancer Treat Rev, 2002, 28(1): 65-74.
[3] Marx RE. A new concept in the treatment of osteoradionecrosis[J]. J Oral Maxillofac Surg, 1983, 41(6): 351-357.
[4] Al-Nawas B, Duschner H, Grötz KA. Early cellular alterations in bone after radiation therapy and its relation to osteoradionecrosis [J]. J Oral Maxillofac Surg, 2004, 62(8): 1045.
[5] Sweeny L, Lancaster WP, Dean NR, et al. Use of recombinant bone morphogenetic protein 2 in free flap reconstruction for osteronecrosis of the mandible [J]. J Oral Maxillofac Surg, 2012, 70(8): 1991-1996.
[6] Xu J, Zheng Z, Fang D, et al. Mesenchymal stromal cell-based treatment of jaw osteoradionecrosis in swine[J]. Cell Transplant, 2012, 21(8): 1679-1686.
[7] 孙勇刚, 孙开华, 王光和, 等. 颌骨及颌周软组织放射性损害的超微结构观察 [J].中华口腔医学杂志,1996,31(3): 147-149.
[8] Eisen MD, Weinstein GS, Chalian A, et al. Morbidity after midline mandibulotomy and radiation therapy [J]. Am J Otolaryngol, 2000, 21(5): 312-317.
[9] Oh HK, Chambers MS, Garden AS, et al. Risk of osteoradionecrosis after extraction of impacted third molars in irradiated head and neck cancer patients [J]. J Oral Maxillofac Surg, 2004, 62(2): 139-144.
[10] Murray CG, Herson J, Daly TE, et al. Radiation necrosis of the mandible: a 10 year study. PartⅡ. Dental factors; onset, duration and management of necrosis [J]. Int J Radiat Oncol Biol Phys, 1980, 6(5): 549-553.
[11] Epsten JB, Wong FL, Stevenson-Moore P. Osteoradionecrosis: clinical experience and a proposal for classification [J]. J Oral Maxillofac Surg, 1987, 45(2): 104-110.
[12] Reuther T, Schuster T, Mende U, et al. Osteoradionecrosis of the jaws as a side effect of radiotherapy of head and neck tumour patients-a report of a thirty year retrospective review [J]. Int J Oral Maxillofac Surg, 2003, 32(3): 289-295.
[13] Beumer J, Harrison R, Sanders B, et al. Osteoradionecrosis: predisposing factors and outcomes of therapy[J]. Head Neck Surg, 1984, 6(4): 819-827.
[14] Schwartz HC, Kagan AR. Osteoradionecrosis of the mandible: scientific basis for clinical staging[J]. Am J Clin Oncol,2002,25(2): 168-171.
[15] Glanzmann C, Grötz KW. Radionecrosis of the mandibula: a retrospective analysis of the incidence and risk factors [J]. Radiother Oncol, 1995, 36(2): 94-100.
[16] Sanger JR, Matloub HS, Yousif NJ, et al. Management of osteoradionecrosis of the mandible[J]. Clin Plast Surg,1993,20(3): 517-530.