Effects of calcium hydroxide and Hoshino’s paste on the proliferation of porcine dental papilla cells

Abstract

Abstract: PURPOSE: To observe the effects of calcium hydroxide and Hoshino’s paste(a mixture of metronidazole, ciprofloxacin, and minocycline) on the proliferation of porcine dental papilla cells (pDPCs), and to provide some theoretical basis for the choice of root canal disinfection drugs for pulp revascularization treatment. METHODS: The 4th generation of pDPCs were treated with several concentrations of (10%,1%,0.1%) and Hoshino’s paste (0.5%, 0.1%, 0.05%) medium for certain time periods. CCK-8 assay was performed to detect the effect of the 2 drugs on cell proliferation. SPSS19.0 software package was used for one-way ANOVA. RESULTS: The effect of Ca(OH)₂ on pDPCs proliferation was fluctuating, but cell proliferation of each Ca(OH)₂ group had no difference after 9 days. Meanwhile, cell proliferation was inhibited by Hoshino’s paste. CONCLUSIONS: Each concentration of in the study has no effect on pDPCs proliferation , while Hoshino’s paste can inhibit cell proliferation.

Key words: Calcium hydroxide, Hoshino’s paste, Procine dental papilla cells Shanghai J Stomatol,2015, ,24(4)419?422.

CLC Number:

R782.1

QIU Si-hui, WANG Jun. Effects of calcium hydroxide and Hoshino’s paste on the proliferation of porcine dental papilla cells[J]. Shanghai Journal of Stomatology, 2015, 24(4): 419-422.

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