Cytotoxicity of a novel endodontic treatment material iRoot BP Plus to human gingival fibroblasts

Abstract

Abstract: PURPOSE: To compare the cytotoxicity of a novel endodontic treatment material iRoot BP Plus and mineral trioxide aggregate (MTA) to human gingival fibroblasts in vitro. METHODS: Cultured human gingival fibroblasts were exposed to multiple concentrations of material elutes (no dilution, 1:2 dilution, and 1:5 dilution). The test material samples were immersed and incubated in the culture medium for 1, 3 or 7 days at 37℃. The proliferation rate was evaluated using methylthiazol tetrazolium (MTT) assay. Cell relative growth rates were presented as . The data was statistically analyzed by factorial design ANOVA using SPSS 20.0 software package. RESULTS: Cell relative growth rates of the eluates of iRoot BP Plus and MTA in different concentrations ranged from 77.31% to 113.82%. The cytotoxicity grade of both materials was 0 or 1 (no cytotoxicity). There was no significant difference in the relative growth rate in different concentrations of iRoot BP plus and MTA eluates under different elution times (F_{concentration*time*material}=1.393, P=0.256). CONCLUSIONS: Both iRoot BP Plus and MTA exhibit minimal level of cytotoxicity.

Key words: iRoot BP Plus, Mineral trioxide aggregate, Cytotoxicity, Human gingival fibroblasts

CLC Number:

R781.05

SHI Shuang¹, BAO Zhi-fan^{1, 2}, CHEN Xu^{1, 2}, ZHANG Dan-dan¹. Cytotoxicity of a novel endodontic treatment material iRoot BP Plus to human gingival fibroblasts[J]. Shanghai Journal of Stomatology, 2014, 23(6): 681-684.

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