Effect of Naringin on proliferation and osteogenic differentiation of bone marrow stromal cells in vitro

Shanghai Journal of Stomatology ›› 2014, Vol. 23 ›› Issue (3): 280-284.

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Effect of Naringin on proliferation and osteogenic differentiation of bone marrow stromal cells in vitro

ZHANG Xue-bin^1,2, LIU Tian-lin², ZHANG Xin¹, TAN Ruan-jun², GE Hai-liang³, HUANG Yuan-liang²

1.Laboratory of Oral Biomedical Science and Translational Medicine, School of Stomatology, Tongji University. Shanghai 200072;
2.Department of Stomatology, the Affiliated East Hospital of Tongji University.Shanghai 200120;
3.Department of Immunology, Shanghai Jiao Tong University School of Medicine. Shanghai 200025, China

Received:2013-10-14 Online:2014-06-20 Published:2014-09-09
Supported by:
Supported by National Natural Science Foundation of China (81070806), Research Fund of Science and Technology Committee of Shanghai Municipality (10JC1413300) and Youth Foundation of Pudong Municipal Commission of Health and Family Planning (PW2011B-13).

Abstract

Abstract: PURPOSE: To investigate the differentiation and osteogenic activity of Naringin-induced bone marrow stromal cells (BMSCs) in dogs. METHODS:BMSCs were separated and cultured in vitro and identified by FCM. Then different concentration of Naringin (1×10-5, 1×10-6, 1×10-7, 1×10-8 and 1×10-9 mol/L) were added to cell culture media to induce BMSCs. The effect of Naringin on BMSCs was evaluated respectively by CCK-8 method and measuring the activity of alkaline phosphatase (ALP). The formation of nodules of calcium was detected by von Kossa staining. The data was analyzed with SPSS20.0 software package. RESULTS: The result of cell-surface marker displayed that the expression of CD34 and CD45 were negative while the expression of CD90 was positive. The values were 0.126%, 0.075% and 95.4%, respectively. Naringin could obviously promote cell proliferation, which exhibited the best effect on proliferation and osteogenic differentiation at concentration of 10-6 mol/L. Calcium nodule (von Kossa) staining was positive. CONCLUSIONS: Naringin-induced bone marrow stromal cells can be differentiated into osteoblasts. Naringin at the concentration of 10-6 mol/L can enhance the proliferation and osteogenic differentiation of BMSCs.

Key words: Naringin, BMSCs, Osteoinduction, Bone tissue engineering

CLC Number:

R782

ZHANG Xue-bin, LIU Tian-lin, ZHANG Xin, TAN Ruan-jun, GE Hai-liang, HUANG Yuan-liang. Effect of Naringin on proliferation and osteogenic differentiation of bone marrow stromal cells in vitro[J]. Shanghai Journal of Stomatology, 2014, 23(3): 280-284.

References

[1] 郭靠山, 李蒙, 李瑞玉. 补肾中药对成骨细胞作用研究进展 [J]. 现代中西医结合杂志, 2012, 21(4): 448-450.
[2] 司徒镇强, 吴军正. 细胞培养[M]. 第2版. 西安: 世界图书出版公司, 2007: 148-150.
[3] Rasini V, Dominici M, Kluba T, et al. Mesenchymal stromal/stem cells markers in the human bone marrow[J]. Cytotherapy, 2013, 15(3): 292-306.
[4] Dominici M, Le Blanc K, Mueller I, et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement[J]. Cytotherapy, 2006, 8(4): 315-317.
[5] Jiang Y, Jahagirdar BN, Reinhardt RL, et al. Pluripotency of mesenchymal stem cells derived from adult marrow[J]. Nature, 2002, 418(6893): 41-49.
[6] Kratchmarova I, Blagoev B, Haack-Sorensen M, et al. Mechanism of divergent growth factor effects in mesenchymal stem cell differentiation [J]. Science, 2005, 308 (5727): 1472-1477.
[7] 胡其勇, 陈莉丽, 王仁飞. 骨碎补柚皮苷对人牙周韧带细胞增殖和成骨分化潜能的影响 [J]. 浙江大学学报(医学版), 2010, 39(1): 79-83.
[8] Malekzadeh R, Hollinger JO, Buck D, et al. Isolation of human osteoblast-like cells and in vitro amplification for tissue engineering [J]. J Periodontol, 1998, 69(11): 1256-1262.
[9] Majumdar MK, Thiede MA, Mosca JD, et al. Phenotypic and functional comparison of marrow derived mesenchymal stem cells (MSCs) and stromal cells [J]. J Cell Physiol, 1998, 176(1): 57-66.
[10] Tondreau T, Lagneaux L, Dejeneffe M, et al. Isolation of BM mesenchymal stem cells by plastic adhesion or negative selection: phenotype, proliferation kinetics and differentiation potential [J]. Cytotherapy, 2004, 6(4): 372-379.
[11] Baddoo M, Hill K, Wilkinson R, et al. Characterization of mesenchymal stem cells isolated from murine bone marrow by negative selection [J]. J Cell Biochem, 2003, 89(6): 1235-1249.
[12] Jacobsen KA, Al-Aql ZS, Wan C, et al. Bone formation during distraction osteogenesis is dependent on both VEGFR1 and VEGFR2 signaling [J]. J Bone Miner Res, 2008, 23 (5): 596-609.
[13] Sch?nau E, Rauch F. Markers of bone and collagen metabolism-problems and perspectives in paediatrics [J]. Horm Res, 1997, 48(Suppl 5): 50-59.
[14] Rawadi G, Vayssière B, Dunn F, et al. BMP-2 controls alkaline phosphatase expression and osteoblast mineralization by a Wnt autocrine loop [J]. J Bone Miner Res, 2003, 18 (10): 1842-1853.
[15] Alborzi A, Mac K, Glackin CA, et al. Endochondral and intramembranous fetal bone development: osteoblastic cell proliferation, and expression of alkaline phosphatase, m-twist, and histone H4 [J]. J Craniofac Genet Dev Biol, 1996, 16(2): 94-106.

Effect of Naringin on proliferation and osteogenic differentiation of bone marrow stromal cells in vitro

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