Detection of the exogenous gene copy number of the transgenic tomato anti-caries vaccine

Shanghai Journal of Stomatology ›› 2013, Vol. 22 ›› Issue (6): 613-617.

• Basic Scientific Study • Previous Articles Next Articles

Detection of the exogenous gene copy number of the transgenic tomato anti-caries vaccine

BAI Guo-hui^1,2, LIU Jian-guo¹, TIAN Yuan¹, CHEN Zhu^1,3, BAI Peng-yuan¹, HAN Qi¹, GU Yu¹, GUAN Xiao-yan¹, WANG Hai-hui¹

1. The Special Key Laboratory of Oral Diseases Research, Higher Education Institution of Guizhou Province, 2. Research Center for Medicine & Biology, Zunyi Medical College. Zunyi 563000; 3. The Stomatological Hospital of Guiyang City. Guiyang 550002, Guizhou Province, China

Received:2013-04-05 Revised:2013-05-10 Online:2013-04-12 Published:2013-04-12
Supported by:
Supported by National Natural Science Foundation of China (30160086, 81260164), Science and Technical Fund of Guizhou Province (LKZ[2011]41)， Project of Technology Innovation Team in Guizhou Province, Leading Academic Discipline Construction Project in Guizhou Province and Excellent Scientific Research Team Cultivation Project in Zunyi Medical College ([2012]12).

Abstract

Abstract: PURPOSE: To detect the exogenous gene copy number of the transgenic tomato anti-caries vaccine by using the SYBR Green real-time PCR. METHODS: Recombinant plasmid pEAC10 and pEPC10 were used as standard to detect genome samples of exogenous gene pacA-ctxB and pacP-ctxB by SYBR green fluorescent quantitation, then the average value was calculated as gene copy number. RESULTS: The copy number of the transgenic tomato carrying pacA-ctxB was 1.3 and the pacP-ctxB was 3.2. CONCLUSIONS: The transgenic tomato plants which have high stability are low-copy transgenic plants.

Key words: Transgenic tomato, Real-time fluorescence quantitative PCR, SYBR Green, Threshold cycle, Copy number

CLC Number:

R781

BAI Guo-hui, LIU Jian-guo, TIAN Yuan, CHEN Zhu, BAI Peng-yuan, HAN Qi, GU Yu, GUAN Xiao-yan, WANG Hai-hui. Detection of the exogenous gene copy number of the transgenic tomato anti-caries vaccine[J]. Shanghai Journal of Stomatology, 2013, 22(6): 613-617.

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Detection of the exogenous gene copy number of the transgenic tomato anti-caries vaccine

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