Use of recombinant human beta-defensin-3 to evaluate the effect of adhesion of Candida albicans on the surface of soft lining material

Shanghai Journal of Stomatology ›› 2013, Vol. 22 ›› Issue (4): 389-392.

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Use of recombinant human beta-defensin-3 to evaluate the effect of adhesion of Candida albicans on the surface of soft lining material

SHI Yong¹, SONG Wei², LIU Xiao-fang¹, GAO Li-min¹, SONG Guang-bao¹

1.Department of Prosthodontics, Guangdong Provincial Stomatological Hospital, Affiliated Hospital of Southern Medical University. Guangzhou 510280; 2. Department of Outpatient Service, Affiliated to the Logistics Department of Guangzhou Military Command. Guangzhou 510080, Guangdong Province, China

Received:2013-01-08 Revised:2013-03-04 Online:2013-08-10 Published:2013-08-10
Supported by:
Supported by Medical Science and Technology Research Fund of Guangdong Province (B2012038).

Abstract

Abstract: PURPOSE: To evaluate the effectiveness of recombinant human beta-defensin-3 (rHBD3) on reduction of Candida albicans from the surface of soft lining materials. METHODS: Specimens made of soft lining materials, which had been contaminated with C. albicans, were immersed in 25 μg/mL rHBD3 and 5.25% sodium hypochlorite (a positive control) for 5, 10, 30 and 60 min. The results were analyzed statistically with SPSS 10.0 software package. Confocal laser scanning microscopy(CLSM) of biofilm-associated C. albicans were treated with rHBD3 in different times. RESULTS: The results confirmed that an immersion time of 30 min or higher yielded a good disinfection effect, both for the experimental group and the positive control. A large number of pathogenic bacteria were killed and the cells were stained red by SYTO9/PI after 30 min. CONCLUSIONS: It is possible that soft lining materials immersed in rHBD3 solution that possesses antifungal activity may be clinically protective.

Key words: Human beta-defensin 3, Antimicrobial activity, C. albicans, Soft lining materials

CLC Number:

R783.6

SHI Yong, SONG Wei, LIU Xiao-fang, GAO Li-min, SONG Guang-bao. Use of recombinant human beta-defensin-3 to evaluate the effect of adhesion of Candida albicans on the surface of soft lining material[J]. Shanghai Journal of Stomatology, 2013, 22(4): 389-392.

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Use of recombinant human beta-defensin-3 to evaluate the effect of adhesion of Candida albicans on the surface of soft lining material

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