应用代谢组学方法分析口臭患者与健康人舌苔代谢物的差异性

doi:10.19439/j.sjos.2023.05.014

上海口腔医学 ›› 2023, Vol. 32 ›› Issue (5): 525-531.doi: 10.19439/j.sjos.2023.05.014

应用代谢组学方法分析口臭患者与健康人舌苔代谢物的差异性

Aviella Nathania Liman^1,*, 朱宏来^2,*, 杨雯洁^1#, 叶玮^1#

1.上海交通大学医学院附属第九人民医院口腔预防科,上海交通大学口腔医学院,国家口腔医学中心,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海市口腔医学研究所,上海 200011;
2.上海市闵行区莘庄社区卫生服务中心,上海 201199

收稿日期:2023-01-29 修回日期:2023-03-29 出版日期:2023-10-25 发布日期:2023-11-03
通讯作者: 叶玮,E-mail: jyyewei@163.com;杨雯洁,E-mail: stellaywj@163.com。^#共同通信作者
作者简介:Aviella Nathania Liman（1995-）,女,硕士,E-mail: avi950825ella@hotmail.com;朱宏来（1978-）,男,学士,主治医师,E-mail: 13671782267@163.com。^*并列第一作者

Metabolomics study on the difference of tongue coating metabolites between patients with intra-oral halitosis and healthy individual

LIMAN Aviella-nathania¹, ZHU Hong-lai², YANG Wen-jie¹, YE Wei¹

1. Department of Preventive Dentistry, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine; College of Stomatology, Shanghai Jiaotong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology; Shanghai Research Institute of Stomatology. Shanghai 200011;
2. Xinzhuang Community Health Service Center, Minhang District. Shanghai 201199, China

Received:2023-01-29 Revised:2023-03-29 Online:2023-10-25 Published:2023-11-03

摘要/Abstract

摘要： 目的：利用非靶向代谢组学方法,分析口源性口臭患者和健康人舌苔代谢物差异,寻找口源性口臭差异显著代谢物作为生物标志物。方法：采用液相色谱质谱技术结合主成分分析法和正交偏最小二乘法-判别分析法,分别对12例口源性口臭患者和12名健康人舌苔样本进行非靶向代谢组学研究,以正交偏最小二乘法-判别分析法模型中变量投影重要性值>1和 t检验P<0.05为标准筛选并确定差异性代谢物。结果：口源性口臭患者和健康人舌苔代谢物存在差异,鉴定到11个差异代谢物,谷氨酸、甘氨酸-苯丙氨酸、色氨酰-脯氨酸、脱氧腺苷、4,5-二氢白色向日葵素、N-乙酰-DL-色氨酸、帕拉米松乙酸酯、环戊醇、(2-己基环戊亚基)肼硫代甲酰胺、L-哌可酸和牛磺酸。其中口源性口臭组中的甘氨酸-苯丙氨酸和N-乙酰-DL-色氨酸表达显著上调,而牛磺酸表达显著下调。结论：口源性口臭患者和健康人的舌苔代谢物存在差异,具有诊断价值的差异代谢物或可作为口源性口臭的诊断标志物。

关键词: 口源性口臭, 舌苔, 代谢组学, 生物标志物

Abstract: PURPOSE: To analyze the difference of metabolites of tongue coating between patients with intra-oral halitosis and healthy individuals by untargeted metabolomics, and to explore significant differences in metabolites of intra-oral halitosis as biomarkers. METHODS: The untargeted metabolomics of tongue coating samples from 12 patients with intra-oral halitosis and 12 healthy individuals were studied by liquid chromatography and mass spectrometry combined with principal component analysis and orthogonal partial least squares discriminant analysis. The value of variable importance in projection >1 and P<0.05 of Student's t test in the orthogonal partial least squares-discriminant analysis model were used as the criteria to screen and determine the differential metabolites. RESULTS: There were differences in the metabolites of tongue coating between patients with intra-oral halitosis and healthy individuals, and 11 different metabolites were identified. They were valyl-arginine, glycine-phenylalanine, tryptophyl-proline, deoxyadenosine, 4,5-dihydroniveusin A, N-acetyl-DL-tryptophan, paramethasone acetate, cyclopentanol, [(2-hexylcyclopentylidene) amino]thiourea, L-pipecolic acid and taurine. In the intra-oral halitosis group, the expressions of Glycine-phenylalanine and N-acetyl-DL-tryptophan were significantly up-regulated, while the expressions of taurine were significantly down-regulated. CONCLUSIONS: There are differences in the metabolites of tongue coating between patients with intra-oral halitosis and healthy individuals. The differential metabolites with diagnostic value may be used as diagnostic markers of intra-oral halitosis.

Key words: Intra-oral Halitosis, Tongue coating, Metabolomics, Biomarker

中图分类号:

R781.9

Aviella Nathania Liman, 朱宏来, 杨雯洁, 叶玮. 应用代谢组学方法分析口臭患者与健康人舌苔代谢物的差异性[J]. 上海口腔医学, 2023, 32(5): 525-531.

LIMAN Aviella-nathania, ZHU Hong-lai, YANG Wen-jie, YE Wei. Metabolomics study on the difference of tongue coating metabolites between patients with intra-oral halitosis and healthy individual[J]. Shanghai Journal of Stomatology, 2023, 32(5): 525-531.

参考文献

[1] van den Broek AM, Feenstra L, de Baat C, et al. A review of the current literature on aetiology and measurement methods of halitosis[J]. J Dent, 2007, 35(8): 627-635.
[2] Olszewska-Czyz I, Sozkes S, Dudzik A.Clinical trial evaluating quality of life in patients with intra-oral halitosis[J]. J Clin Med, 2022, 11(2): 326-340.
[3] Quirynen M, Dadamio J, Velde S, et al.Characteristics of 2000 patients who visited a halitosis clinic[J]. J Clin Periodontol, 2010, 36(11): 970-975.
[4] Muniz FW, Friedrich SA, Silveira CF, et al.The impact of chewing gum on halitosis parameters: a systematic review[J]. J Breath Res, 2017, 11(1): 014001.
[5] Seemann R, Conceicao MD, Filippi A, et al.Halitosis management by the general dental practitioner-results of an international consensus workshop[J]. J Breath Res, 2014, 8(1): 017101.
[6] Seerangaiyan K, Jüch F, Winkel EG.Tongue coating: its characteristics and role in intra-oral halitosis and general health-a review[J]. J Breath Res, 2018, 12(3): 034001.
[7] Halket JM, Waterman D, Przyborowska AM, et al.Chemical derivatization and mass spectral libraries in metabolic profiling by GC/MS and LC/MS/MS[J]. J Exp Bot, 2005, 56(410): 219-243.
[8] Kiseleva O, Kurbatov I, Ilgisonis E, et al.Defining blood plasma and serum metabolome by GC-MS[J]. Metabolites, 2021, 12(1): 15-45.
[9] Rosenberg M, McCulloch CA. Measurement of oral malodor: current methods and future prospects[J]. J Periodontol, 1992, 63(9): 776-782.
[10] Rosenberg M, Kulkarni GV, Bosy A, et al.Reproducibility and sensitivity of oral malodor measurements with a portable sulphide monitor[J]. J Dent Res, 1991, 70(11): 1436-1440.
[11] Wang X, Sun G, Feng T, et al.Sodium oligomannate therapeutically remodels gut microbiota and suppresses gut bacterial amino acids-shaped neuroinflammation to inhibit Alzheimer's disease progression[J]. Cell Res, 2019, 29(10): 787-803.
[12] Xie M, Chen W, Lai X, et al.Metabolic responses and their correlations with phytochelatins in Amaranthus hypochondriacus under cadmium stress[J]. Environ Pollut, 2019, 252(Pt B): 1791-1800.
[13] Zhang YR, Zhao HT, Guo YY, et al.Relationship between thick or greasy tongue-coating microbiota and tongue diagnosis in patients with primary liver cancer[J]. Front Microbiol, 2022, 13: 903616.
[14] Sun ZM, Zhao J, Qian P, et al.Metabolic markers and microecological characteristics of tongue coating in patients with chronic gastritis[J]. BMC Complement Altern Med, 2013, 13: 227-236.
[15] Hao YM, Yuan X, Yan J, et al.Metabolomic markers in tongue-coating samples from damp phlegm pattern patients of coronary heart disease and chronic renal failure[J]. Dis Markers, 2019, 2019: 4106293.
[16] Hao YM, Zhang RL, Morris R, et al.Metabolome and microbiome alterations in tongue coating of gastric precancerous lesion patients[J]. Expert Rev Gastroenterol Hepatol, 2021, 15(8): 949-963.
[17] Duarte da Silva K, Vargas-Ferreira F, Damaso Bertoldi A, et al. Oral mucosal lesions in pregnant women: a population-based study[J]. Oral Dis, 2022, 28(7): 1891-1900.
[18] 肖金禾, 金信妍, 樊英怡, 等. 哺乳期急性乳腺炎黄腻苔患者舌苔特异性菌群特征研究[J]. 中医杂志, 2021, 62(13): 1150-1155.
[19] Jacob M, Gu X, Luo X, et al.Metabolomics distinguishes DOCK8 deficiency from atopic dermatitis: towards a biomarker discovery[J]. Metabolites, 2019, 9(11): 274-288.
[20] Matos Do Canto L, Marian C, Varghese RS, et al. Metabolomic profiling of breast tumors using ductal fluid[J]. Int J Oncol, 2016, 49(6): 2245-2254.
[21] Sirianni AC, Jiang J, Zeng J, et al.N-acetyl-l-tryptophan, but not N-acetyl-d-tryptophan, rescues neuronal cell death in models of amyotrophic lateral sclerosis[J]. J Neurochem, 2015, 134(5): 956-968.
[22] Dion MZ, Leiske D, Sharma VK, et al.Mitigation of oxidation in therapeutic antibody formulations: a biochemical efficacy and safety evaluation of N-acetyl-tryptophan and L-Methionine[J]. Pharm Res, 2018, 35(11): 222.
[23] El Agouza IM, Eissa SS, El Houseini MM, et al.Taurine: a novel tumor marker for enhanced detection of breast cancer among female patients[J]. Angiogenesis, 2011, 14(3): 321-330.
[24] Ma X, Wang Y, Wu H, et al.Periodontal health related-inflammatory and metabolic profiles of patients with end-stage renal disease: potential strategy for predictive, preventive, and personalized medicine[J]. EPMA J, 2021, 12(2): 117-128.
[25] Seerangaiyan K, Winkelhoff AV, Harmsen H, et al.The tongue microbiome in healthy subjects and patients with intra-oral halitosis[J]. J Breath Res, 2017, 11(3): 036010.
[26] Acar B, Berker E, Tan Ç, et al.Effects of oral prophylaxis including tongue cleaning on halitosis and gingival inflammation in gingivitis patients-a randomized controlled clinical trial[J]. Clin Oral Investig, 2019, 23(4): 1829-1836.

应用代谢组学方法分析口臭患者与健康人舌苔代谢物的差异性

Metabolomics study on the difference of tongue coating metabolites between patients with intra-oral halitosis and healthy individual

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