炎症微环境对人牙周膜成纤维细胞增殖与成骨分化的影响

doi:10.19439/j.sjos.2022.03.004

上海口腔医学 ›› 2022, Vol. 31 ›› Issue (3): 243-247.doi: 10.19439/j.sjos.2022.03.004

炎症微环境对人牙周膜成纤维细胞增殖与成骨分化的影响

董家辰, 束蓉

上海交通大学医学院附属第九人民医院牙周病科,上海交通大学口腔医学院,国家口腔医学中心, 国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海 200011

收稿日期:2021-01-19 修回日期:2021-03-16 出版日期:2022-06-25 发布日期:2022-07-07
通讯作者: 束蓉,E-mail: shurong123@hotmail.com
作者简介:董家辰(1987-),男,硕士,主治医师, E-mail: dongjiachensky@163.com
基金资助:
上海交通大学医学院附属第九人民医院交叉基金资助（JYJC201904）

The effect of inflammation on proliferation and osteogenic differentiation of periodontal ligament cells

DONG Jia-chen, SHU Rong

Department of Periodontology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China

Received:2021-01-19 Revised:2021-03-16 Online:2022-06-25 Published:2022-07-07

摘要/Abstract

摘要： 目的: 观察脂多糖模拟的炎症微环境对体外培养的人牙周膜成纤维细胞（periodontal ligament cells, PDLCs）增殖与成骨分化的影响。方法: 体外培养鉴定人牙周膜成纤维细胞,分别用浓度为0、0.1、10 μg/mL脂多糖作用于细胞,采用MTT法观察脂多糖对PDLCs增殖的影响,运用实时定量PCR和Western印迹法检测脂多糖模拟的炎症微环境对PDLCs成骨分化的影响。采用SPSS 13.0软件包对数据进行统计学分析。结果: 0.1 μg/mL脂多糖促进PDLCs增殖,增强成骨标志物mRNA和蛋白的表达;10 μg/mL脂多糖可抑制PDLCs的增殖及碱性磷酸酶、RUNX2、Ⅰ型胶原、骨形态发生蛋白2的表达,其结果有统计学意义。结论: 高浓度脂多糖（10 μg/mL）模拟的炎症微环境对PDLCs增殖及成骨分化有抑制作用,低浓度脂多糖（0.1 μg/mL）对PDLCs的增殖和成骨分化有促进作用。

关键词: 人牙周膜成纤维细胞, 炎症微环境, 脂多糖, 成骨分化

Abstract: PURPOSE: To investigate the effects of inflammatory microenvironment on proliferation and osteogenic differentiation of periodontal ligament cells(PDLCs) in vitro. METHODS: Human PDLCs were isolated and characterized. MTT was used to investigate the proliferation rate of PDLCs under different concentration of lipopolysaccharide（LPS）. The PDLCs' osteogenic differentiation was investigated using real-time PCR and Western blot. The date were statistically analyzed with SPSS 13.0 software package. RESULTS: Treatment with 0.1 μg/mL LPS increased proliferation of PDLCs and enhanced the expression of osteogenic gene and protein. The proliferation of PDLCs and expression of alkaline phosphatase(ALP), RUNX2, Collagen-I, BMP2 were significantly decreased by 10 μg/mL LPS. CONCLUSIONS: The inflammatory microenvironment (10 μg/mL LPS) inhibits the proliferation and osteogenic differentiation of human PDLCs.

Key words: Periodontal ligament cells, Inflammatory microenvironment, Lipopolysaccharide, Osteogenic differentiation

中图分类号:

R781.4

董家辰, 束蓉. 炎症微环境对人牙周膜成纤维细胞增殖与成骨分化的影响[J]. 上海口腔医学, 2022, 31(3): 243-247.

DONG Jia-chen, SHU Rong. The effect of inflammation on proliferation and osteogenic differentiation of periodontal ligament cells[J]. Shanghai Journal of Stomatology, 2022, 31(3): 243-247.

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炎症微环境对人牙周膜成纤维细胞增殖与成骨分化的影响

The effect of inflammation on proliferation and osteogenic differentiation of periodontal ligament cells

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