人牙周膜成纤维细胞来源外泌体对大鼠延期牙再植术后牙根吸收的调控作用

doi:10.19439/j.sjos.2022.03.002

上海口腔医学 ›› 2022, Vol. 31 ›› Issue (3): 232-236.doi: 10.19439/j.sjos.2022.03.002

人牙周膜成纤维细胞来源外泌体对大鼠延期牙再植术后牙根吸收的调控作用

尹悦, 陶硕, 张旗

同济大学口腔医学院牙体牙髓病学教研室,上海牙组织再生与修复工程技术研究中心,上海 200072

收稿日期:2021-06-04 修回日期:2021-08-08 出版日期:2022-06-25 发布日期:2022-07-07
通讯作者: 张旗,E-mail：qizhang@tongji.edu.cn
作者简介:尹悦（1993-）,女,硕士,E-mail：2213799894@qq.com
基金资助:
国家自然科学基金（81870760、81570966）; 中央高校基本科研业务费专项资金（22120190217）

Effects of human periodontal ligament fibroblast-derived exosomes on reducing rat root resorption after delayed tooth replantation

YIN Yue, TAO Shuo, ZHANG Qi

Department of Endodontics, School of Stomatology, Tongji University; Shanghai Engineering Research Center of Tooth Restoration and Regeneration. Shanghai 200072, China

Received:2021-06-04 Revised:2021-08-08 Online:2022-06-25 Published:2022-07-07

摘要/Abstract

摘要： 目的: 探讨健康的人牙周膜成纤维细胞（hPDLFs）来源外泌体对大鼠延期牙再植术后牙根吸收的作用及其可能机制。方法: 分离提取hPDLFs来源的外泌体并鉴定。选择30只6周龄雄性SD大鼠,随机分为对照组和外泌体组,建立上颌第一磨牙延期牙再植术模型,牙脱位30 min后植回牙槽窝。对照组牙周膜局部注射40 μL汉克斯平衡盐溶液（HBSS）,实验组牙周膜局部注射40 μL含外泌体的HBSS。术后1、2、4周收样,苏木精-伊红（H-E）染色观察牙根表面吸收陷窝,抗酒石酸酸性磷酸酶（TRAP）染色观察破骨细胞数量,免疫组织化学染色观察牙周膜内骨保护素（OPG）的表达。采用SPSS 17.0软件包对数据进行统计学处理。结果: 鉴定表明提取的细胞外囊泡为外泌体。与对照组相比,hPDLFs来源的外泌体减少了延期牙再植术后牙根吸收陷窝的数量,降低TRAP阳性破骨细胞的表达（P<0.05）,促进牙周膜内OPG的表达（P<0.05）。结论: 延期牙再植术后,hPDLFs来源的外泌体减少了破骨细胞的数量,促进牙周膜内OPG表达,减少了再植术后的牙根吸收。

关键词: 外泌体, 牙根吸收, 延期牙再植, 骨保护素

Abstract: PURPOSE: To explore the effect of healthy human periodontal ligament fibroblasts (hPDLFs) -derived exosomes on tooth resorption after delayed tooth replantation in rats and its possible mechanism. METHODS: The exosomes derived from hPDLFs were isolated and identified in thirty six-week-old SD rats and randomly divided into control group and exosome group. The right maxillary first molar was extracted to establish a delayed tooth replantation model. The dislocated teeth were implanted back into the alveolar fossa after 30 minutes. 40 μL Hanks' balanced salt solution (HBSS) were injected into the periodontal tissue, and the experimental group was injected with 40 μL HBSS containing exosomes. The rats were sacrificed at 1, 2, and 4 weeks. Hematoxylin-eosin (H-E) staining was used to observe tooth resorption. Tartrate-resistant acid phosphatase(TRAP) staining was used to observe the number of osteoclasts. The expression of osteoprotegerin(OPG) in periodontal ligament was detected by immunohistochemical staining. The differences in distribution of each genotype were analyzed with SPSS 17.0 software package. RESULTS: The identification experiment showed that extracellular vesicles were exosomes. hPDLFs-derived exosomes inhibited root resorption after delayed tooth replantation, reduced the expression of TRAP-positive osteoclasts (P<0.05), and promoted expression of OPG in periodontal ligament (P<0.05). CONCLUSIONS: After delayed tooth replantation, PDLFs-derived exosomes reduce the number of osteoclasts, promote OPG expression in the periodontal ligament, and reduce tooth root resorption after replantation.

Key words: Exosomes, Root resorption, Delayed tooth replantation, Osteoprotegerin

中图分类号:

尹悦, 陶硕, 张旗. 人牙周膜成纤维细胞来源外泌体对大鼠延期牙再植术后牙根吸收的调控作用[J]. 上海口腔医学, 2022, 31(3): 232-236.

YIN Yue, TAO Shuo, ZHANG Qi. Effects of human periodontal ligament fibroblast-derived exosomes on reducing rat root resorption after delayed tooth replantation[J]. Shanghai Journal of Stomatology, 2022, 31(3): 232-236.

参考文献

[1] Osmanovic A, Halilovic S, Kurtovic-Kozaric A, et al.Evaluation of periodontal ligament cell viability in different storage media based on human PDL cell culture experiments-a systematic review[J]. Dent Traumatol, 2018, 34(6): 384-393.
[2] Liu H, Peng X, Sun H, et al.Clinical and histopathological characterization of root resorption in replanted teeth: two case reports[J]. Medicine (Baltimore), 2020, 99(3): e18869.
[3] Zhan X, Zhang C, Dissanayaka WL, et al.Storage media enhance osteoclastogenic potential of human periodontal ligament cells via RANKL-independent signaling[J]. Dent Traumatol, 2013, 29(1): 59-65.
[4] Matos Fde S, Godolphim Fde J, Correia AM, et al.Effect of laser photobiomodulation on the periodontal repair process of replanted teeth[J]. Dent Traumatol, 2016, 32(5): 402-408.
[5] Lee JS, Kim SK, Gruber R, et al.Periodontal healing by periodontal ligament fiber with or without cells: a preclinical study of the decellularized periodontal ligament in a tooth replantation model[J]. J Periodontol, 2020, 91(1): 110-119.
[6] Liu Y, Du H, Wang Y, et al.Osteoprotegerin-knockout mice developed early onset root resorption[J]. J Endod, 2016, 42(10): 1516-1522.
[7] Altanerova U, Benejova K, Altanerova V, et al.Dental pulp mesenchymal stem/stromal cells labeled with iron sucrose release exosomes and cells applied intra-nasally migrate to intracerebral glioblastoma[J]. Neoplasma, 2016, 63(6): 925-933.
[8] Zhang S, Chuah SJ, Lai RC, et al.MSC exosomes mediate cartilage repair by enhancing proliferation, attenuating apoptosis and modulating immune reactivity[J]. Biomaterials, 2018, 156: 16-27.
[9] Wang M, Li J, Ye Y, et al.SHED-derived conditioned exosomes enhance the osteogenic differentiation of PDLSCs via Wnt and BMP signaling in vitro[J]. Differentiation, 2020, 111: 1-11.
[10] Zhao M, Dai W, Wang H, et al.Periodontal ligament fibroblasts regulate osteoblasts by exosome secretion induced by inflammatory stimuli[J]. Arch Oral Biol, 2019, 105: 27-34.
[11] Sato K, Muramatsu T, Tsuchiya Y, et al.Proliferation, migration and apoptosis of periodontal ligament cells after tooth replantation[J]. Oral Dis, 2010, 16(3): 263-268.
[12] Console L, Scalise M, Indiveri C.Exosomes in inflammation and role as biomarkers[J]. Clin Chim Acta, 2019, 488: 165-171.
[13] Yang D, Zhang W, Zhang H, et al.Progress, opportunity, and perspective on exosome isolation - efforts for efficient exosome-based theranostics[J]. Theranostics, 2020, 10(8): 3684-3707.
[14] Tang YT, Huang YY, Zheng L, et al.Comparison of isolation methods of exosomes and exosomal RNA from cell culture medium and serum[J]. Int J Mol Med, 2017, 40(3): 834-844.
[15] Helwa I, Cai JW, Drewry MD, et al.A comparative study of serum exosome isolation using differential ultracentrifugation and three commercial reagents[J]. PloS One, 2017, 12(1): e0170628.
[16] De Brier N, Dorien O, Borra V, et al.Storage of an avulsed tooth prior to replantation: a systematic review and meta-analysis[J]. Dent Traumatol, 2020, 36(5): 453-476.
[17] Najeeb S, Siddiqui F, Khurshid Z, et al.Effect of bisphosphonates on root resorption after tooth replantation-a systematic review[J]. Dent Traumatol, 2017, 33(2): 77-83.
[18] Liu M, Sun Y, Zhang Q.Emerging role of extracellular vesicles in bone remodeling[J]. J Dent Res, 2018, 97(8): 859-868.
[19] Chew JRJ, Chuah SJ, Teo KYW, et al.Mesenchymal stem cell exosomes enhance periodontal ligament cell functions and promote periodontal regeneration[J]. Acta Biomater, 2019, 89: 252-264.
[20] Liu D, Fang Y, Rao Y, et al.Synovial fibroblast-derived exosomal microRNA-106b suppresses chondrocyte proliferation and migration in rheumatoid arthritis via down-regulation of PDK4[J]. J Mol Med (Berl), 2020, 98(3): 409-423.
[21] Vogt BF, Souza CE, Silva DN, et al.Evaluation of two formulations containing mineral trioxide aggregate on delayed tooth replantation: relevance of RANKL/RANK/OPG system[J]. Odontology, 2016, 104(2): 211-219.
[22] Boyce BF.Advances in the regulation of osteoclasts and osteoclast functions[J]. J Dent Res, 2013, 92(10): 860-867.
[23] Boyle WJ, Simonet WS, Lacey DL.Osteoclast differentiation and activation[J]. Nature, 2003, 423(6937): 337-342.

人牙周膜成纤维细胞来源外泌体对大鼠延期牙再植术后牙根吸收的调控作用

Effects of human periodontal ligament fibroblast-derived exosomes on reducing rat root resorption after delayed tooth replantation

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	霍美玲, 雷颖, 辛欣, 张颖, 王瑞梅. 2种矫治器对轻中度Ⅰ类错𬌗患者前牙牙根及牙槽骨的影响[J]. 上海口腔医学, 2024, 33(4): 407-410.
[2]	王静, 孙一凡, 路茜, 庞淑婷. 微种植钉支抗与平面导板在下前牙过长型深覆矫治中的效果评价[J]. 上海口腔医学, 2024, 33(3): 301-305.
[3]	周子芊, 陈振琦. 上颌窦底对上颌第一磨牙近中移动的影响[J]. 上海口腔医学, 2022, 31(6): 661-667.
[4]	林怡君, 鄢洁雅, 王天鸽, 周志捷, 廖盛轩, 毛丽霞, 刘加强. 减数正畸治疗中中切牙牙根及牙槽骨形态变化的锥形束CT测量分析[J]. 上海口腔医学, 2022, 31(2): 211-216.
[5]	葛伶伶, 吕倩, 白雪, 吴邵鸿, 姜喜玲. 青少年与成年人正畸治疗后上颌切牙牙根吸收程度比较[J]. 上海口腔医学, 2021, 30(2): 187-190.
[6]	赵力如, 毛家奇, 赵丙姣, 陈静. 牙周膜干细胞来源外泌体的生物学特性分析[J]. 上海口腔医学, 2019, 28(4): 343-348.
[7]	刘安琪, 钱玉芬. 上颌尖牙阻生原因及其对周围结构的影响[J]. 上海口腔医学, 2017, 26(6): 684-688.
[8]	孙小娜, 宋爱梅, 杨丕山. 布洛芬辅助治疗重度慢性牙周炎的短期疗效及对龈沟液中细胞因子的影响[J]. 上海口腔医学, 2017, 26(5): 557-560.
[9]	史新连, 胡碧波, 任曼曼, 喻文彬, 邓辉. 低氧调控大鼠骨髓间充质干细胞OPG/RANKL mRNA的表达[J]. 上海口腔医学, 2017, 26(3): 258-262.
[10]	胡琴, 徐小淋, 周建萍, 戴红卫. 釉基质蛋白衍生物对大鼠牙移动后复发和牙根修复的影响[J]. 上海口腔医学, 2017, 26(2): 156-161.
[11]	王冠, 杨璐, 张玉峰, 罗三莲, 郑纪伟. 无托槽隐形矫治器和直丝弓矫治器对切牙牙根吸收的影响[J]. 上海口腔医学, 2017, 26(1): 121-124.
[12]	刘筠，郭宏铭. 自锁托槽与传统托槽导致正畸牙根吸收的锥形束CT比较[J]. 上海口腔医学, 2016, 25(2): 238-241.
[13]	李振兴, 陈传俊. 雌激素降低对大鼠髁突软骨ERα、ERβ、OPG和RANKL表达的影响[J]. 上海口腔医学, 2015, 24(4): 437-441.
[14]	孙晓琳,周延民,赵静辉,郑玲,杨婷婷. 富血小板纤维蛋白对体外培养的成骨细胞生物学特性的影响[J]. 上海口腔医学, 2015, 24(1): 61-64.
[15]	曾婧;王旭霞;贾玉龙;马丹;王胜林;张君;. 帕米膦酸钠对大鼠正畸牙移动过程中破牙骨质细胞及破骨细胞分化因子表达的影响 [J]. 上海口腔医学, 2012, 21(5): 495-500.