顽固性复发性阿弗他溃疡患者外周血Th17细胞与免疫球蛋白及补体水平的变化

doi:10.19439/j.sjos.2017.05.011

上海口腔医学 ›› 2017, Vol. 26 ›› Issue (5): 521-525.doi: 10.19439/j.sjos.2017.05.011

顽固性复发性阿弗他溃疡患者外周血Th17细胞与免疫球蛋白及补体水平的变化

邹俐琳¹, 王文梅¹, 刘雅菁¹, 蒋廷旺², 王蓓丽¹

1.南京大学医学院附属口腔医院·南京市口腔医院,江苏南京 210008;；
2.常熟市医学检验所,江苏常熟 215500

收稿日期:2017-01-11 修回日期:2017-04-19 出版日期:2017-10-25 发布日期:2017-11-23
通讯作者: 王文梅,E-mail：wangwenmei0102@163.com
作者简介:邹俐琳(1986-),女,在读硕士研究生,E-mail：107810140@qq.com
基金资助:
国家自然科学基金（81570978、81400521）; 江苏省临床医学科技专项（BL2014018）

Alteration of Th17 cells, immunoglobins and complements levels in peripheral blood of patients with refractory recurrent aphthous ulcer

ZOU Li-lin¹, WANG Wen-mei¹, LIU Ya-jing¹, JIANG Ting-wang², WANG Bei-li¹

1.Department of Oral Medicine, Nanjing Stomatological Hospital, Medical School of Nanjing University. Nanjing 210008;
2. Changshu Medical Testing Institution. Changshu 215500, Jiangsu Province, China

Received:2017-01-11 Revised:2017-04-19 Online:2017-10-25 Published:2017-11-23

摘要/Abstract

摘要： 目的探讨Th17细胞与免疫球蛋白及补体在顽固性复发性阿弗他溃疡（顽固性RAU）患者外周血中的变化。方法收集30例顽固性RAU患者和21例健康志愿者的外周血,采用流式细胞术检测Th17细胞的数量;采用免疫散射比浊法检测患者外周血血清免疫球蛋白IgA、IgG、IgM、IgE和补体C3、C4的水平,分析Th17细胞水平的改变以及Th17细胞与血清体液免疫水平之间的关系。采用SPSS17.0软件包对数据进行统计学分析。结果顽固性RAU患者外周血Th17细胞（CD3⁺CD8^-IL-17⁺）占CD4⁺T细胞的百分率显著高于健康对照组（P<0.01）,免疫球蛋白IgG、IgA与IgE的水平显著高于健康对照组（P<0.05）,IgM和补体C3、C4的水平与健康对照组相比无显著差异（P>0.05）;Th17细胞与IgA的表达呈明显的负相关（r=-0.477,P<0.01）。结论顽固性RAU患者外周血中Th17细胞和血清免疫球蛋白及补体水平存在差异,可能均参与了顽固性RAU的发生与发展,且Th17细胞与体液免疫之间的改变可能存在某种联系。

关键词: 顽固性复发性阿弗他溃疡, Th17细胞, 体液免疫, 免疫球蛋白, 补体

Abstract: PURPOSE: To observe the levels of Th17 cells, immunoglobins and complements in peripheral blood of patients with refractory recurrent aphthous ulcer (refractory RAU) patients. METHODS: Totally, 30 patients with refractory RAU and 21 healthy controls were enrolled into this study. Flow cytometry was performed to determine the frequency of Th17 cells in the peripheral blood. Immune scatter turbidimetry was adopted to measure the levels of IgA, IgG, IgM, IgE, C3 and C4 in the peripheral blood. Meanwhile, the correlations between the frequency of Th17 cells and the levels of immunoglobins or complements were analyzed. SPSS17.0 software package was used to analyze the date. RESULTS: Compared with the control group, the percentage of Th17 cells (CD3⁺CD8^-IL17⁺) in all CD4⁺T cells was significantly increased in the refractory RAU group (P<0.01). Similarly, the levels of IgG, IgA, IgE in the refractory RAU group were significantly higher than those in the control group (P<0.05),but there was no significant difference in the levels of IgM, C3 and C4 between the refractory RAU group and control group. There was negative correlation between the frequency of Th17 cells and the level of IgA (r=-0.477, P<0.01). CONCLUSIONS: The levels of Th17 cells, immunoglobins and complements in refractory RAU patients are significantly different from healthy human, which may play an important role in the pathogenesis of refractory RAU. There may be some relationship between Th17 cells and the change of humoral immunity in refractory RAU.

Key words: Refractory recurrent aphthous ulcer, Th17 cells, Humoral immunity, Immunoglobins, Complements

中图分类号:

R781.5

邹俐琳, 王文梅, 刘雅菁, 蒋廷旺, 王蓓丽. 顽固性复发性阿弗他溃疡患者外周血Th17细胞与免疫球蛋白及补体水平的变化[J]. 上海口腔医学, 2017, 26(5): 521-525.

ZOU Li-lin, WANG Wen-mei, LIU Ya-jing, JIANG Ting-wang, WANG Bei-li. Alteration of Th17 cells, immunoglobins and complements levels in peripheral blood of patients with refractory recurrent aphthous ulcer[J]. Shanghai Journal of Stomatology, 2017, 26(5): 521-525.

参考文献

[1] Rogers RS 3rd. Complex aphthosis [J]. Adv Exp Med Biol, 2003, 528: 311-316.
[2] 晋素丽, 耿发云. 灵杆菌多糖治疗顽固性复发性阿弗他溃疡的临床观察 [J]. 现代口腔医学杂志, 2008, 22(2): 217.
[3] 中华口腔医学会口腔黏膜病专业委员会. 复发性阿弗他溃疡的诊疗指南（试行）[J]. 中华口腔医学杂志, 2012, 47(7): 402-404.
[4] Baccaglini L, Lalla RV, Bruce AJ, et al. Urban legends: recurrent aphthous stomatitis [J]. Oral Dis, 2011, 17(8): 755-770.
[5] Slebioda Z, Szponar E, Kowalska A. Etiopathogenesis of recurrent aphthous stomatitis and the role of immunologic aspects: literature review [J]. Arch Immunol Ther Exp (Warsz), 2014, 62(3): 205-215.
[6] Almoznino G, Zini A, Mizrahi Y, et al. Elevated serum IgE in recurrent aphthous stomatitis and associations with disease characteristics [J]. Oral Dis, 2014, 20(4): 386-394.
[7] Brozovic S, Vucicevic-Boras V, Bukovic D. Serum IgA, IgG, IgM and salivary IgA in recurrent aphthous ulceration[J]. Coll Antropol, 2001, 25(2): 633-637.
[8] Ozyurt K, Celik A, Sayarlioglu M, et al. Serum Th1, Th2 and Th17 cytokine profiles and alpha-enolase levels in recurrent aphthous stomatitis [J]. J Oral Pathol Med, 2014, 43(9): 691-695.
[9] Lewkowicz N, Kur B, Kurnatowska A, et al. Expression of Th1/Th2/Th3/Th17-related genes in recurrent aphthous ulcers [J]. Arch Immunol Ther Exp (Warsz), 2011, 59(5): 399-406.
[10] Harrington LE, Mangan PR, Weaver CT. Expanding the effector CD4 T-cell repertoire: the Th17 lineage [J]. Curr Opin Immunol, 2006, 18(3): 349-356.
[11] 陈谦明. 口腔黏膜病学[M]. 第4版. 北京: 人民卫生出版社, 2012: 64-69.
[12] Hsu HC, Yang P, Wang J, et al. Interleukin 17-producing T helper cells and interleukin 17 orchestrate autoreactive germinal center development in autoimmune BXD2 mice [J]. Nat Immunol, 2008, 9(2): 166-175.
[13] Weaver CT, Harrington LE, Mangan PR, et al. Th17: an effector CD4 T cell lineage with regulatory T cell ties [J]. Immunity, 2006, 24(6): 677-688.
[14] 谢三祥, 丰琳, 朱声荣, 等. 转录因子RORγτ和FOXP3在口腔扁平苔藓病损组织中的表达及意义[J]. 上海口腔医学, 2014, 23(4): 472-476.
[15] Ghoreschi K, Laurence A, Yang XP, et al. T helper 17 cell heterogeneity and pathogenicity in autoimmune disease [J]. Trends Immunol, 2011, 32(9): 395-401.
[16] Hemdan NY, Birkenmeier G, Wichmann G, et al. Interleukin-17-producing T helper cells in autoimmunity[J]. Autoimmun Rev, 2010, 9(11): 785-792.
[17] Kohno M, Tsutsumi A, Matsui H, et al. Interleukin-17 gene expression in patients with rheumatoid arthritis[J]. Mod Rheumatol, 2008, 18(1):15-22.
[18] Benedetti G, Miossec P. Interleukin 17 contributes to the chronicity of inflammatory diseases such as rheumatoid arthritis [J]. Eur J Immunol, 2014, 44(2): 339-347.
[19] Jadidi-Niaragh F, Mirshafiey A. Th17 cell, the new player of neuroinflammatory process in multiple sclerosis[J]. Scand J Immunol, 2011, 74(1): 1-13.
[20] Komiyama Y, Nakae S, Matsuki T, et al. IL-17 plays an important role in the development of experimental autoimmune encephalomyelitis [J]. J Immunol, 2006, 177(1): 566-573.
[21] Roeleveld DM, van Nieuwenhuijze AE, van den Berg WB, et al. The Th17 pathway as a therapeutic target in rheumatoid arthritis and other autoimmune and inflammatory disorders[J]. Bio Drugs, 2013, 27(5):439-452.
[22] 王栋, 李言君, 付爱丽, 等. Treg与IL-17在复发性口腔溃疡中表达的相关性研究 [J]. 实用口腔医学杂志, 2012, 28(4): 466-469.
[23] 林佳如, 樊均明. 白细胞介素17在IgA肾病中引起IgA1糖基化异常的作用研究 [J]. 重庆医学, 2016, 45(28): 3907-3909.
[24] 刘瑜, 赵宏霞, 曹敏, 等. 哮喘患儿血清白细胞介素-4、白细胞介素-17和干扰素-γ水平的检测 [J]. 中国实验诊断学, 2013, 17(4): 735-737.
[25] Pan HF, Ye DQ, Li XP. Type 17 T-helper cells might be a promising therapeutic target for systemic lupus erythematosus [J]. Nat Clin Pract Rheumatol, 2008, 4(7): 352-353.
[26] Garrett-Sinha LA, John S, Geffen SL. IL-17 and the Thl7 lineage in systemic lupus erythematosus[J]. Curr Opin Rheumatol, 2008, 20(5): 519-525.
[27] Maddur MS, Vani J, Hegde P, et al. Inhibition of differentiation, amplification, and function of human TH17 cells by intravenous immunoglobulin [J]. J Allergy Clin Immunol, 2011, 127(3): 823-830.

顽固性复发性阿弗他溃疡患者外周血Th17细胞与免疫球蛋白及补体水平的变化

Alteration of Th17 cells, immunoglobins and complements levels in peripheral blood of patients with refractory recurrent aphthous ulcer

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 2

编辑推荐

Metrics

本文评价

[1]	郎小英, 李颂. RORγT在人根尖肉芽肿和根尖囊肿中的表达及意义[J]. 上海口腔医学, 2014, 23(4): 465-471.
[2]	张青, 方利华, 平金良. IgG4相关硬化性腮腺炎1例报告及文献复习[J]. 上海口腔医学, 2013, 22(4): 473-477.